LOCUS       62056_22115        4998 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4998
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2777..2793)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2801..2817)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2825..2855)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2870..2891)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3179..3767)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3941..4798)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4799..4903)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ggagatcggt acttcgcgaa
      421 tgcgtcgaga taccgggtgt gggagggggg tggggggtgg gggtgggggg tatcggggaa
      481 actggggaag atgggagagc tggaggagag tcgtggggtc cgaggagcac ctcggcatct
      541 ggagtctggc aggaatgttg aaggactgag gggctagctc aggcagagca aaggcatcgc
      601 aaaggctgga aaacatcgga gtgaagctga agggcctgag ctagggttgg agaggaatgg
      661 ggagccagac attcatcccg gttacttttg gttacaggac gtggcggctg gtcggataaa
      721 ggggagctgc tgggaagggt tcgaccccag acctgggcag tgaaggtata gctggcagca
      781 gtgggcaggt gaggaccgcc gtctgctggg caggtgagtc tccttcttct ctcttggcct
      841 cgctccactg accttctaaa cgaaggttta gagagggggc ctggtgagaa gaagcggctg
      901 gcctcgcagc agaatggcac atagaaaggc aggatagtta gcaaaggaga catcgtctcg
      961 gggggagccg agacagaagg aggctggggg accattggcg accccaggtg gaaagagctc
     1021 ttagagagaa gaaagaagag gtgcagggtt gccagtaaag actgaggccg ctgcctccag
     1081 ggaggtgata ggagtccttg gagacagtgg cagagaccat gggatccagc aagaacagaa
     1141 gcattctagg ctggggtcaa acagggcaag atggggtcac aagacacaga tgggtcccca
     1201 gccgccacaa catcccaccc accgtaattc acttagaaga aggttcaaga gtggctctgg
     1261 caaagtccca agtttgccag agcctcaata actggagaat ggaaaagaag ggcagtgcag
     1321 ggtgtcacca gaaggggagt gggggctgca ggtatcggac tccagaggga ttttacagca
     1381 aggaggctgc agtgggtcca gcctgcagac acaccattcc catgaggcac tgcggcccag
     1441 ggactggtgc ggaaagggcc cacagtggac ttggtacact gtatgcccta accgctcagt
     1501 ccctgggtct ggcatgacag acagaacatt tccaggggag tcaagggcac aggatgaagc
     1561 cagacgaggc gaggcaggcg gggcagaatg aatgagtttc tagggaggga ggttgggtgc
     1621 aggtagagcg agtagctggg gtggtgagcc agggaggcac tggcctccag agtccgtggc
     1681 caaggagggc cttgcgggcg gcgacggagc agtgatcggt gtctcgaaga gctcggactg
     1741 gagactaggc caggtctcca gcagaggtgg atgtgcctgc cagtcactga aggcgaggat
     1801 gacaggtgtg gtcaatgtga cagaaagaca tgacatggtc cggtgtgatg gagaggacag
     1861 aagggcagtc atccagcctt cttgaacacc atgggctggc gccttgtcgt agaagccgtc
     1921 tgttctttca cttttcccaa agagctaaca cttctctgct gctctctgga tcctcctccc
     1981 cctaccttga accctcaaga tgaaagaaat ggtgctaccc agctcatgtc tgggcctttg
     2041 aatccgggga cttctttaag tccgtctcgt tctgaatcaa gaagatgctg caatcagaac
     2101 cactacacta cctgcctcag gaatctgctc caaggtgaag ctgaaagaac agatggtgtc
     2161 aacattttga aagagcagac tcatagcacc cacccacccc tgagaatcca tcttcatggc
     2221 caactctgcc tgacccggga gaccaccacc cacatcatcc tggagccaag cctctacccc
     2281 gggatgactt catcatctcc ctcctgtctt tttcttcttc ctcctttcct gtaattctgt
     2341 ttctttcctt ttgttccttc cttgcttgag agactcaaag cacccgtgac tctgtttccc
     2401 catttacccc cttttgaatt tgcactaagt cgattgcact ggtttggagt cccggagata
     2461 gctttgagtc tctccgtatg aatgtataca gcgagtgtgt aaacctcttt ggcaatgctg
     2521 ccccagtacc cacctgtcgt ccatctccgt ctgagggcaa ctgggtgtgg ccgtgtgctt
     2581 gaggcctcgc cttcccctcg cctagtctgg aagcagttcc atcataaagt gttcaacatg
     2641 ccctacttca tcctttgccc ctcctcacca gggcctcacc agaggtcctg ggtccatcaa
     2701 taaatacagt tacagtcata tcggatgccg ggaccgacga gtgcagaggc gtgcaagcga
     2761 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     2821 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     2881 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     2941 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     3001 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     3061 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     3121 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     3181 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     3241 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     3301 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     3361 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     3421 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     3481 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     3541 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     3601 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     3661 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     3721 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     3781 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     3841 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     3901 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     3961 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     4021 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     4081 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     4141 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     4201 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     4261 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     4321 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     4381 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     4441 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     4501 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     4561 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     4621 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     4681 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     4741 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     4801 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     4861 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     4921 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     4981 tcacgaggcc ctttcgtc
//
