LOCUS       V013255                 2948 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013255
VERSION     V013255
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..2948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             447..671
                     /gene="E"
                     /label="Envelope small membrane protein"
                     /note="Envelope small membrane protein from Severe acute
                     respiratory syndrome coronavirus 2. Accession#: P0DTC4"
     primer_bind     complement(727..743)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(751..767)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(775..805)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(820..841)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1129..1717)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1891..2748)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2749..2853)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
      421 tgcatctaga tatcggatcc cgacacatgt actcattcgt ttcggaagag acaggtacgt
      481 taatagttaa tagcgtactt ctttttcttg ctttcgtggt attcttgcta gttacactag
      541 ccatccttac tgcgcttcga ttgtgtgcgt actgctgcaa tattgttaac gtgagtcttg
      601 taaaaccttc tttttacgtt tactctcgtg ttaaaaatct gaattcttct agagttcctg
      661 atcttctggt ctaagtgtcg ggcccgtcga ctgcagaggc ctgcatgcaa gcttggcgta
      721 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
      781 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
      841 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
      901 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
      961 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     1021 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     1081 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     1141 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     1201 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     1261 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     1321 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     1381 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     1441 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     1501 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     1561 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     1621 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     1681 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     1741 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     1801 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     1861 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     1921 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     1981 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     2041 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     2101 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     2161 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     2221 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     2281 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     2341 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     2401 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     2461 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     2521 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     2581 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     2641 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     2701 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     2761 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     2821 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     2881 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     2941 ctttcgtc
//
