LOCUS       V016498                 3662 bp    DNA     circular SYN 13-JUN-2020
DEFINITION  Exported.
ACCESSION   V016498
VERSION     V016498
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Suzuki M.
  TITLE     Bacterial ARG expression libraries
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Suzuki M.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) Contact:Masato Suzuki National Institute of
            Infectious Diseases, AMR Research Center; 4-2-1 Aobacho,
            Higashimurayama, Tokyo 189-0002, Japan
REFERENCE   3  (bases 1 to 3662)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (19-MAY-2020) Contact:Masato Suzuki National Institute of Infectious
            Diseases, AMR Research Center; 4-2-1 Aobacho, Higashimurayama, Tokyo
            189-0002, Japan"
FEATURES             Location/Qualifiers
     source          1..3662
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        450..478
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     CDS             873..1403
                     /gene="aadB"
                     /label="2''-aminoglycoside nucleotidyltransferase"
                     /note="2''-aminoglycoside nucleotidyltransferase from
                     Escherichia coli. Accession#: P0AE04"
     primer_bind     complement(1441..1457)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1465..1481)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1489..1519)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1534..1555)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1843..2431)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2605..3462)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3463..3567)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatcacac
      421 cgtggaaacg gatgaaggca cgaacccagt tgacataagc ctgttcggtt cgtaaactgt
      481 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
      541 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttgta cagtctatgc
      601 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
      661 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaggcatc acaaagtaca
      721 gcatcatggc ttgttatgac tgtttttttg tacagtctat gcctcgggca tccaagcagc
      781 aagcgcgtta cgccgtgggt cgatgtttga tgttatggag cagcaacgat gttacgcagc
      841 agggcagtcg ccctaaaaca aagttaggcc gcatggacac aacgcaggtc acattgatac
      901 acaaaattct agctgcggca gatgagcgaa atctgccgct ctggatcggt gggggctggg
      961 cgatcgatgc acggctaggg cgtgtaacac gcaagcacga tgatattgat ctgacgtttc
     1021 ccggcgagag gcgcggcgag ctcgaggcaa tagttgaaat gctcggcggg cgcgtcatgg
     1081 aggagttgga ctatggattc ttagcggaga tcggggatga gttacttgac tgcgaacctg
     1141 cttggtgggc agacgaagcg tatgaaatcg cggaggctcc gcagggctcg tgcccagagg
     1201 cggctgaggg cgtcatcgcc gggcggccag tccgttgtaa cagctgggag gcgatcatct
     1261 gggattactt ttactatgcc gatgaagtac caccagtgga ctggcctaca aagcacatag
     1321 agtcctacag gctcgcatgc acctcactcg gggcggaaaa ggttgaggtc ttgcgtgccg
     1381 ctttcaggtc gcgatatgcg gcctaaatcc caatggcgcg ccgagcttgg cgtaatcatg
     1441 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     1501 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     1561 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     1621 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     1681 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     1741 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     1801 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     1861 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     1921 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     1981 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     2041 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     2101 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     2161 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     2221 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     2281 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     2341 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     2401 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     2461 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     2521 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     2581 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     2641 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     2701 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     2761 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     2821 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     2881 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     2941 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     3001 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     3061 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     3121 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     3181 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     3241 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     3301 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     3361 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     3421 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     3481 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     3541 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     3601 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     3661 tc
//
