LOCUS       V015502                 3581 bp    DNA     circular SYN 13-JUN-2020
DEFINITION  Exported.
ACCESSION   V015502
VERSION     V015502
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Suzuki M.
  TITLE     Bacterial ARG expression libraries
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Suzuki M.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) Contact:Masato Suzuki National Institute of
            Infectious Diseases, AMR Research Center; 4-2-1 Aobacho,
            Higashimurayama, Tokyo 189-0002, Japan
REFERENCE   3  (bases 1 to 3581)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (19-MAY-2020) Contact:Masato Suzuki National Institute of Infectious
            Diseases, AMR Research Center; 4-2-1 Aobacho, Higashimurayama, Tokyo
            189-0002, Japan"
FEATURES             Location/Qualifiers
     source          1..3581
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        450..478
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     CDS             738..1322
                     /gene="aacA4"
                     /label="Aminoglycoside N(6')-acetyltransferase type 1"
                     /note="Aminoglycoside N(6')-acetyltransferase type 1 from
                     Klebsiella pneumoniae. Accession#: P19650"
     primer_bind     complement(1360..1376)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1384..1400)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1408..1438)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1453..1474)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1762..2350)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2524..3381)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3382..3486)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatcacac
      421 cgtggaaacg gatgaaggca cgaacccagt tgacataagc ctgttcggtt cgtaaactgt
      481 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
      541 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttgta cagtctatgc
      601 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
      661 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaggcatc acaaagtaca
      721 gcatcatgag caacgcaaaa acaaagttag gcatcacaaa gtacagcatc gtgaccaaca
      781 gcaacgattc cgtcacactg cgcctcatga ctgagcatga ccttgcgatg ctctatgagt
      841 ggctaaatcg atctcatatc gtcgagtggt ggggcggaga agaagcacgc ccgacacttg
      901 ctgacgtaca ggaacagtac ttgccaagcg ttttagcgca agagtccgtc actccataca
      961 ttgcaatgct gaatggagag ccgattgggt atgcccagtc gtacgttgct cttggaagcg
     1021 gggacggacg gtgggaagaa gaaaccgatc caggagtacg cggaatagac cagttactgg
     1081 cgaatgcatc acaactgggc aaaggcttgg gaaccaagct ggttcgagct ctggttgagt
     1141 tgctgttcaa tgatcccgag gtcaccaaga tccaaacgga cccgtcgccg agcaacttgc
     1201 gagcgatccg atgctacgag aaagcggggt ttgagaggca aggtaccgta accaccccat
     1261 atggtccagc cgtgtacatg gttcaaacac gccaggcatt cgagcgaaca cgcagtgatg
     1321 cctaaatccc aatggcgcgc cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     1381 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     1441 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     1501 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     1561 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     1621 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     1681 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     1741 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     1801 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     1861 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     1921 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     1981 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     2041 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     2101 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     2161 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     2221 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     2281 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     2341 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     2401 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     2461 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     2521 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     2581 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     2641 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     2701 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     2761 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     2821 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     2881 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     2941 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     3001 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     3061 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     3121 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     3181 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     3241 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     3301 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     3361 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     3421 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     3481 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     3541 attaacctat aaaaataggc gtatcacgag gccctttcgt c
//
