LOCUS       V016311                 3518 bp    DNA     circular SYN 13-JUN-2020
DEFINITION  Exported.
ACCESSION   V016311
VERSION     V016311
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Suzuki M.
  TITLE     Bacterial ARG expression libraries
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Suzuki M.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2020) Contact:Masato Suzuki National Institute of
            Infectious Diseases, AMR Research Center; 4-2-1 Aobacho,
            Higashimurayama, Tokyo 189-0002, Japan
REFERENCE   3  (bases 1 to 3518)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (19-MAY-2020) Contact:Masato Suzuki National Institute of Infectious
            Diseases, AMR Research Center; 4-2-1 Aobacho, Higashimurayama, Tokyo
            189-0002, Japan"
FEATURES             Location/Qualifiers
     source          1..3518
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        450..478
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     CDS             726..1259
                     /gene="aac"
                     /label="Aminoglycoside 2'-N-acetyltransferase"
                     /note="Aminoglycoside 2'-N-acetyltransferase from
                     Providencia stuartii. Accession#: Q52424"
     primer_bind     complement(1297..1313)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1321..1337)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1345..1375)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1390..1411)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1699..2287)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2461..3318)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3319..3423)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatcacac
      421 cgtggaaacg gatgaaggca cgaacccagt tgacataagc ctgttcggtt cgtaaactgt
      481 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
      541 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttgta cagtctatgc
      601 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
      661 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaggcatc acaaagtaca
      721 gcatcatggg catagaatac cgcagtctgc ataccagcca attgacactg agtgaaaaag
      781 aagcgcttta cgatttatta attgaaggtt ttgaaggcga tttttcgcat gacgatttcg
      841 cgcacacttt aggtggaatg cacgtcatgg cttttgatca acaaaaattg gttggtcatg
      901 ttgcaattat tcaacgccat atggccctag ataatacgcc tatctctgta gggtatgttg
      961 aagcgatggt agttgaacaa agttatcgtc gccaaggtat tgggcggcaa ttgatgctgc
     1021 aaaccaataa aattatagct tcgtgttatc aattagggct gctgtcggct tcagatgatg
     1081 gacaaaaatt gtatcattcg gttggatggc aaatctggaa aggtaagttg tttgaattga
     1141 aacaagggag ctatatccgt tctattgaag aagaaggcgg agtcatgggc tggaaagcgg
     1201 atggtgaggt tgattttacc gcttcgcttt actgtgattt tcgtggcggt gatcagtggt
     1261 aaatcccaat ggcgcgccga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     1321 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     1381 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     1441 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     1501 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     1561 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     1621 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     1681 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     1741 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1801 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     1861 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     1921 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     1981 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2041 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2101 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     2161 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2221 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2281 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     2341 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     2401 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     2461 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     2521 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     2581 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     2641 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     2701 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     2761 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     2821 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     2881 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     2941 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     3001 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     3061 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     3121 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     3181 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     3241 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     3301 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     3361 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     3421 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     3481 aacctataaa aataggcgta tcacgaggcc ctttcgtc
//
