LOCUS       62056_22065        3914 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3914)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(723..1535)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1693..1709)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1717..1733)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1741..1771)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1786..1807)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2095..2683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2857..3714)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3715..3819)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ccccggatcc gtcgacctgc
      421 aggggggggg gggcgctgag gtctgcctcg tgaagaaggt gttgctgact cataccaggc
      481 ctgaatcgcc ccatcatcca gccagaaagt gagggagcca cggttgatga gagctttgtt
      541 gtaggtggac cagttggtga ttttgaactt ttgctttgcc acggaacggt ctgcgttgtc
      601 gggaagatgc gtgatctgat ccttcaactc agcaaaagtt cgatttattc aacaaagccg
      661 ccgtcccgtc aagtcagcgt aatgctctgc cagtgttaca accaattaac caattctgat
      721 tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata
      781 ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat
      841 aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct
      901 attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact
      961 gaatccggtg agaatggcaa aagcttatgc atttctttcc agacttgttc aacaggccag
     1021 ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc
     1081 gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa
     1141 tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat
     1201 tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca
     1261 tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt
     1321 agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac
     1381 aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca
     1441 ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc
     1501 ctcgagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg
     1561 taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag
     1621 agattttgag acacaacgtg gctttccccc ccccccctgc aggtcgacgg atccggggaa
     1681 ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     1741 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     1801 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     1861 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     1921 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     1981 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     2041 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     2101 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     2161 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     2221 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     2281 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     2341 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     2401 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     2461 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     2521 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     2581 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     2641 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     2701 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     2761 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     2821 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     2881 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     2941 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     3001 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     3061 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     3121 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     3181 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     3241 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     3301 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     3361 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     3421 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     3481 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     3541 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     3601 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     3661 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     3721 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     3781 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     3841 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     3901 gaggcccttt cgtc
//
