LOCUS       V012971                 4890 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V012971
VERSION     V012971
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        541..886
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             901..1290
                     /gene="RAT5"
                     /label="Histone H2A.6"
                     /note="Histone H2A.6 from Arabidopsis thaliana. Accession#:
                     Q9LD28"
     CDS             1303..2010
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     terminator      2030..2282
                     /label="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2291..2307)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2781..2885
                     /label="AmpR promoter"
     CDS             2886..3743
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      3917..4505
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    4793..4814
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4829..4859
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4867..4883
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc cccagattag
       61 ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg
      121 tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa
      181 ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat
      241 atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc
      301 aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat
      361 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt
      421 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
      481 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
      541 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
      601 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
      661 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
      721 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      781 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      841 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctaga
      901 atggctggtc gtggaaaaac tcttggatcc ggtggggcga agaaagctac atctcggagt
      961 agcaaagccg gtcttcaatt cccggtgggt cgtatcgctc gtttcttaaa agccggtaaa
     1021 tacgccgaac gtgttggtgc cggtgctccg gtttatctcg ccgccgttct cgaatatttg
     1081 gccgccgagg ttcttgaatt agctggaaac gcagcaagag acaacaagaa gacacgtatt
     1141 gttcctcgtc acattcagct tgcggtcaga aacgatgagg agctaagcaa gcttcttgga
     1201 gatgtgacga ttgctaatgg aggagtgatg cctaacatcc acaatctcct tctccctaag
     1261 aaggctggtg cttcaaagcc tcaggaagat ctcgaggtcg acatggtgag caagggcgag
     1321 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     1381 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     1441 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     1501 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     1561 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     1621 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     1681 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     1741 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     1801 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     1861 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     1921 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     1981 tccaccggcg gcatggacga gctgtacaag taagagctcg aatttccccg atcgttcaaa
     2041 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     2101 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     2161 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     2221 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
     2281 tcgggaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     2341 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     2401 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt
     2461 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
     2521 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     2581 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     2641 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     2701 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     2761 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     2821 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     2881 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     2941 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     3001 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     3061 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     3121 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     3181 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     3241 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     3301 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     3361 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     3421 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     3481 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     3541 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     3601 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     3661 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     3721 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     3781 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     3841 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     3901 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3961 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     4021 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     4081 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     4141 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     4201 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     4261 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     4321 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     4381 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4441 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4501 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4561 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     4621 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     4681 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     4741 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     4801 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     4861 gtggaattgt gagcggataa caatttcaca
//
