LOCUS       40924_45143        3474 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUC19RP12, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Posfai G, Kolisnychenko V, Bereczki Z, Blattner FR.
  TITLE     Markerless gene replacement in Escherichia coli stimulated by a 
            double-strand break in the chromosome
  JOURNAL   Nucleic Acids Res. 27 (22), 4409-4415 (1999)
  PUBMED    10536150
REFERENCE   2  (bases 1 to 3474)
  AUTHORS   Posfai G, Kolisnychenko V, Bereczki Z, Blattner FR.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-1999) Institute of Biochemistry, Biol. Res. Center
            Hung. Acad.Sci., Temesvari krt.,62, Szeged H-6726, Hungary
REFERENCE   3  (bases 1 to 3474)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3474)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1999"; volume: "27"; issue: "22"; pages: 
            "4409-4415"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-JUL-1999) Institute of Biochemistry, Biol. Res. Center Hung. 
            Acad.Sci., Temesvari krt.,62, Szeged H-6726, Hungary"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        450..505
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     CDS             519..1211
                     /gene="SCEI"
                     /label=SCEI
                     /note="Intron-encoded endonuclease I-SceI from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c). 
                     Accession#: P03882"
     primer_bind     complement(1253..1269)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1277..1293)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1301..1331)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1346..1367)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      1652
                     /label=ColE1
                     /note="ColE1"
     rep_origin      complement(1655..2243)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2417..3274)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3275..3379)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
      421 cctctagaca tcattaattc ctaatttttg ttgacactct atcattgata gagttatttt
      481 accactccct atcagtgata gagaaaagtg aaatgcatca aaaaaaccag gtaatgaacc
      541 tgggtccgaa ctctaaactg ctgaaagaat acaaatccca gctgatcgaa ctgaacatcg
      601 aacagttcga agcaggtatc ggtctgatcc tgggtgatgc ttacatccgt tctcgtgatg
      661 aaggtaaaac ctactgtatg cagttcgagt ggaaaaacaa agcatacatg gaccacgtat
      721 gtctgctgta cgatcagtgg gtactgtccc cgccgcacaa aaaacaacgt gttaaccacc
      781 tgggtaacct ggtaatcacc tggggcgccc agactttcaa acaccaagct ttcaacaaac
      841 tggctaacct gttcatcgtt aacaacaaaa aaaccatccc gaacaacctg gttgaaaact
      901 acctgacccc gatgtctctg gcatactggt tcatggatga tggtggtaaa tgggattaca
      961 acaaaaactc taccaacaaa tcgatcgtac tgaacaccca gtctttcact ttcgaagaag
     1021 tagaatacct ggttaagggt ctgcgtaaca aattccaact gaactgttac gtaaaaatca
     1081 acaaaaacaa accgatcatc tacatcgatt ctatgtctta cctgatcttc tacaacctga
     1141 tcaaaccgta cctgatcccg cagatgatgt acaaactgcc gaacactatc tcctccgaaa
     1201 ctttcctgaa ataataagtc gactgcaggc atgcaagctt ggcgtaatca tggtcatagc
     1261 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     1321 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     1381 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     1441 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     1501 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     1561 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     1621 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     1681 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     1741 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     1801 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     1861 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     1921 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     1981 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     2041 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     2101 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     2161 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     2221 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     2281 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     2341 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     2401 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     2461 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     2521 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     2581 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     2641 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     2701 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     2761 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     2821 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     2881 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     2941 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     3001 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     3061 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     3121 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     3181 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     3241 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     3301 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     3361 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     3421 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc
//
