LOCUS       .                       3894 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    106..127
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..172
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..220
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(1277..1293)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        1805..1833
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     CDS             2160..2393
                     /label="TpR"
                     /note="E. coli plasmid-associated dihydrofolate reductase"
     rep_origin      3124..3712
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttat
      241 ggattcattt cctgagatag aaatagctga atataaagtt tttgatgaaa gtaataataa
      301 tgatgataac gtattaaaca tttcttatgg cgttgatgaa aactatcttg atggtgtggg
      361 ggtatcaatc gcttcagttg tattaaacaa taatatcccg ctcgcttttc acattatttg
      421 tgactcatac tccccgtgtt ttgtaaaata tatagagcgt ttagccgtac agcatcacat
      481 aaaaatttct ctttatctta ttaaagtaga aagccttgag gtattgcctc aaactaaagt
      541 atggtcgaga gcaatgtatt ttcgtttatt tgctttcgat tatctcagca agaaggtaaa
      601 taccttactt tatttggatg ccgatgttgt atgcaaagga tctttgcaag atcttttaca
      661 gcttgatctg acagagaaga tcgctgcggt cgtaaaagat gttgattcca tccagaataa
      721 ggtaaatgag agattaagcg cttttaattt acaaggtggt tattttaact ccggcgtggt
      781 ttttgttaac ctgaaattat ggaaagagaa tgccttaacc aaaaaggcat ttttactttt
      841 ggcaggtaaa gaggctgact cttttaaata tcccgatcag gatgttttga atattctcct
      901 acaggataaa gtcatttttc taccgcgacc gtataatact atttatacta ttaaaagtga
      961 gttgaaagat aagtcacata aaaaatatag caatataatt aatgataata ctattttaat
     1021 tcattatacg ggcgctacaa aaccatggca tgcctgggca aattatcctt cagttatcta
     1081 ttataaaaat gcacgactga actcgccctg gaaagatttt cccgcaaaag atgcgcgtac
     1141 catagtcgaa tttaagaagc gatataaaca tcttctcgtg caaggtcatt attttaaagg
     1201 ccttctggct ggaagcgcat atctttatcg taaacttttc cacaaataag gatccccggg
     1261 taccgagctc gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     1321 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     1381 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga
     1441 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
     1501 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     1561 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     1621 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     1681 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     1741 caggtggcac ttttcgggga aatgtgacta gtggaacgaa atcgatgagc tcgcacgaac
     1801 ccagttgaca taagcctgtt cggttcgtaa actgtaatgc aagtagcgta tgcgctcacg
     1861 caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat
     1921 ggcttgttat gactgttttt ttgtacagtc tagcctcggg catccaagct agctaagcgc
     1981 gttacgccgt gggtcgatgt ttgatgttat ggaacagcaa cgatgttacg cagcagggta
     2041 gtcgccctaa aacaaagtta ggcagccgtt gtgctggtgc tttctagtag ttgttgtggg
     2101 gtaggcagtc agagctcgat ttgcttgtcg ccataataga ttcacaagaa ggattcgaca
     2161 tgggtcaaag tagcgatgaa gccaacgctc ccgttgcagg gcagtttgcg cttcccctga
     2221 gtgccacctt tggcttaggg gatcgcgtac gcaagaaatc tggtgccgct tggcagggtc
     2281 aagtcgtcgg ttggtattgc acaaaactca ctcctgaagg ctatgcggtc gagtccgaat
     2341 cccacccagg ctcagtgcaa atttatcctg tggctgcact tgaacgtgtg gcctaaagta
     2401 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     2461 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     2521 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     2581 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     2641 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     2701 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     2761 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     2821 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     2881 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     2941 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     3001 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     3061 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3121 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3181 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3241 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     3301 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3361 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3421 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     3481 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     3541 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     3601 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     3661 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     3721 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     3781 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     3841 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aaga
//
