LOCUS       V013971                 2796 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013971
VERSION     V013971
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..2796
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..176
                     /label="J23119(SpeI) promoter"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
     CDS             196..369
                     /gene="mcjA"
                     /label="Microcin J25"
                     /note="Microcin J25 from Escherichia coli. Accession#:
                     Q9X2V7"
     primer_bind     complement(400..416)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        890..994
                     /label="AmpR promoter"
     CDS             995..1852
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      2026..2614
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg cttgacagct agctcagtcc taggtataat actagttttc
      181 acacaggaaa cagctatgat taagcatttt cattttaata aactgtcttc tggtaaaaaa
      241 aataatgttc catctcctgc aaagggggtt atacaaataa aaaaatcagc atcgcaactc
      301 acaaaaggtg gtgcaggaca tgtgcctgag tattttgtgg ggattggtac acctatatct
      361 ttctatggct gaggatcccc gggtaccgag ctcgaattca ctggccgtcg ttttacaacg
      421 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
      481 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
      541 cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
      601 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
      661 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
      721 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
      781 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
      841 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      901 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      961 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     1021 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     1081 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     1141 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     1201 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     1261 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     1321 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     1381 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     1441 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     1501 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     1561 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     1621 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     1681 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     1741 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     1801 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     1861 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     1921 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     1981 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     2041 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     2101 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     2161 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     2221 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     2281 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     2341 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     2401 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     2461 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2521 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2581 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     2641 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     2701 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     2761 cgagcgcagc gagtcagtga gcgaggaagc ggaaga
//
