LOCUS       62056_22025        3184 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..176
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
     RBS             182..204
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        1278..1382
                     /label=AmpR promoter
     CDS             1383..2240
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2414..3002
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg cttgacagct agctcagtcc taggtataat actagtttca
      181 cattgtttaa ctttaagaag gagattacgc catgtaccac ccggaaccgc gcgtggcggc
      241 gatcatcgcc tcccacgagc acccggagtt catcgtcaac gtgaaggaga ccggcaaggt
      301 cctgctggtc aactacaagg atatcgacaa cctcaccgtc accagcatcg gtgcggcgcc
      361 gttcctccac gacggcggct gggacagcag ccaccgctac ttcatgaccg ccgccaacaa
      421 ctccaacaag gttgccgtga tcgactccaa ggaccgtcgc ctgtcggccc tggtcgacgt
      481 cggcaagacc ccgcacccgg ggcgtggcgc caacttcgtg catcccaagt acggcccggt
      541 gtggagcacc agccacctgg gcgacggcag catctcgctg atcggcaccg atccgaagaa
      601 ccatccgcag tacgcctgga agaaagtcgc cgaactacag ggccagggcg gcggctcgct
      661 gttcatcaag acccatccga agtcctcgca cctatacgtg gacaccacct tcaaccccga
      721 cgccaggatc agccagagcg tcgcggtgtt cgacctgaag aacctcgacg ccaagtacca
      781 ggtgctgccg atcgccgaat gggccgatct cggcgaaggc gccaagcggg tggtgcagcc
      841 cgagtacaac aagcgcggcg atgaagtctg gttctcggtg tggaacggca agaacgacag
      901 ctccgcgctg gtggtggtgg acgacaagac cctgaagctc aaggccgtgg tcaaggaccc
      961 gcggctgatc accccgaccg gcaagttcaa ctgacatatg gtgcactctc agtacaatct
     1021 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     1081 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     1141 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     1201 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     1261 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     1321 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     1381 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     1441 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     1501 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     1561 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     1621 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     1681 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     1741 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     1801 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     1861 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     1921 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     1981 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     2041 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     2101 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     2161 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     2221 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     2281 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     2341 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     2401 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     2461 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     2521 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     2581 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     2641 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     2701 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     2761 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     2821 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2881 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2941 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     3001 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     3061 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     3121 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     3181 aaga
//
