LOCUS       62056_21995        4290 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..172
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     CDS             226..1875
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1894..1910)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2384..2488
                     /label=AmpR promoter
     CDS             2489..3346
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3520..4108
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 atgaagcttg catggtcgac tctagaggat ccaccggtcg ccaccatgga agacgctaaa
      241 aacatcaaaa aaggtccggc tccgttctac ccgctggaag acggtaccgc tggtgaacag
      301 ctgcacaaag ctatgaaacg ttacgctctg gttccgggta ccatcgcttt caccgacgct
      361 cacatcgaag ttgacatcac ctacgctgaa tacttcgaaa tgtctgttcg tctggctgaa
      421 gctatgaaac gttacggtct gaacaccaac caccgtatcg ttgtttgctc tgaaaactct
      481 ctgcagttct tcatgccggt tctgggtgct ctgttcatcg gtgttgctgt tgctccggct
      541 aacgacatct acaacgaacg tgaactgctg aactctatgg gtatctctca gccgaccgtt
      601 gttttcgttt ctaaaaaagg tctgcagaaa atcctgaacg ttcagaaaaa actgccgatc
      661 atccagaaaa tcatcatcat ggactctaaa accgactacc agggtttcca gtctatgtac
      721 accttcgtta cctctcacct gccgccgggt ttcaacgaat acgacttcgt tccggaatct
      781 ttcgaccgtg acaaaaccat cgctctgatc atgaactctt ctggttctac cggtctgccg
      841 aaaggtgttg ctctgccgca ccgtaccgct tgcgttcgtt tctctcacgc tcgtgacccg
      901 atcttcggta accagatcat cccggacacc gctatcctgt ctgttgttcc gttccaccac
      961 ggtttcggta tgttcaccac cctgggttac ctgatctgcg gtttccgtgt tgttctgatg
     1021 taccgtttcg aagaagaact gttcctgcgt tctctgcagg actacaaaat ccagtctgct
     1081 ctgctggttc cgaccctgtt ctctttcttc gctaaatcta ccctgatcga caaatacgac
     1141 ctgtctaacc tgcacgaaat cgcttctggt ggtgctccgc tgtctaaaga agttggtgaa
     1201 gctgttgcta aacgtttcca cctgccgggt atccgtcagg gttacggtct gaccgaaacc
     1261 acctctgcta tcctgatcac cccggaaggt gacgacaaac cgggtgctgt tggtaaagtt
     1321 gttccgttct tcgaagctaa agttgttgac ctggacaccg gtaaaaccct gggtgttaac
     1381 cagcgtggtg aactgtgcgt tcgtggtccg atgatcatgt ctggttacgt taacaacccg
     1441 gaagctacca acgctctgat cgacaaagac ggttggctgc actctggtga catcgcttac
     1501 tgggacgaag acgaacactt cttcatcgtt gaccgtctga aatctctgat caaatacaaa
     1561 ggttaccagg ttgctccggc tgaactggaa tctatcctgc tgcagcaccc gaacatcttc
     1621 gacgctggtg ttgctggtct gccggacgac gacgctggtg aactgccggc tgctgttgtt
     1681 gttctggaac acggtaaaac catgaccgaa aaagaaatcg ttgactacgt tgcttctcag
     1741 gttaccaccg ctaaaaaact gcgtggtggt gttgttttcg ttgacgaagt tccgaaaggt
     1801 ctgaccggta aactggacgc tcgtaaaatc cgtgaaatcc tgatcaaagc taaaaaaggt
     1861 ggtaaaatcg ctgtttaaaa aatttaagaa ttcactggcc gtcgttttac aacgtcgtga
     1921 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     1981 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     2041 tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     2101 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     2161 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     2221 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     2281 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     2341 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     2401 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     2461 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     2521 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     2581 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     2641 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     2701 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     2761 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     2821 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     2881 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     2941 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     3001 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     3061 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     3121 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     3181 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     3241 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     3301 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     3361 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     3421 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     3481 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3541 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     3601 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     3661 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     3721 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     3781 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     3841 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     3901 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     3961 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4021 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4081 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4141 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     4201 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     4261 cagcgagtca gtgagcgagg aagcggaaga
//
