LOCUS       62056_22000        3112 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3112
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..172
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(716..732)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1206..1310
                     /label=AmpR promoter
     CDS             1311..2168
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2342..2930
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttat
      241 gtccctccca tccccgtacc ccggtctgcc gccgctcaag aagcgcgcca cgacccgcac
      301 cattttcatc cacgccgccg atactccccc caacatggac atcggcgcgc gtgacatcca
      361 ccgctggcat gtggtcgata accactgggc cgcgattggc taccacttcg tcatccggcg
      421 cgatggcacc gtggagggtg gccgccccca caacaccgtg ggcgcccaag tggcgagccg
      481 caactccgac tccatcggca tctgcttggt gggcggcaag ggaaagttct ccggcgagga
      541 cccctacgcc cactacaccg aggcgcagat catgtccctc gtggtgacca tccggtcgct
      601 catggaaatc tacccgaacg tgaacgtgct gggtcaccgc gatgcggacc ccggcaagca
      661 gtgcccgagc ttcgacgcta aggcgtggtg gctgaaggtg gccgccgagg aattcactgg
      721 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
      781 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
      841 cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc
      901 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
      961 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     1021 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     1081 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     1141 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     1201 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     1261 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     1321 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     1381 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     1441 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     1501 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     1561 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     1621 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     1681 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     1741 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     1801 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     1861 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     1921 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     1981 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     2041 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     2101 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     2161 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     2221 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     2281 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     2341 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     2401 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     2461 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     2521 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     2581 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     2641 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     2701 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     2761 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     2821 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     2881 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     2941 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     3001 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     3061 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa ga
//
