LOCUS       62056_21980        6876 bp DNA     circular SYN 18-NOV-2020
DEFINITION  Cloning vector pUC19 ACMV DNA-B 1.5mer, complete sequence.
ACCESSION   MT856194
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6876)
  AUTHORS   Hoyer JS, Fondong VN, Dallas MM, Aimone CD, Deppong DO, Duffy S, 
            Hanley-Bowdoin L.
  TITLE     Deeply Sequenced Infectious Clones of Key Cassava Begomovirus 
            Isolates from Cameroon
  JOURNAL   Microbiol Resour Announc 9 (46), e00802-20 (2020)
  PUBMED    33184153
REFERENCE   2  (bases 1 to 6876)
  AUTHORS   Hoyer JS, Fondong VN, Dallas MM, Aimone CD, Deppong DO, Duffy S, 
            Hanley-Bowdoin L.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-AUG-2020) Ecology, Evolution, and Natural Resources, 
            Rutgers University, 14 College Farm Rd, New Brunswick, NJ 08901, USA
REFERENCE   3  (bases 1 to 6876)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol 
            Resour Announc"; date: "2020"; volume: "9"; issue: "46"; pages: 
            "e00802-20"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-AUG-2020) Ecology, Evolution, and Natural Resources, Rutgers 
            University, 14 College Farm Rd, New Brunswick, NJ 08901, USA"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Benchling v. 2019-01-22
            Sequencing Technology :: Illumina; Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      171..759
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    1047..1068
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1083..1113
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1121..1137
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1145..1161
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1174..2656
                     /note="duplicated part of ACMV DNA-B partial tandem dimer, 
                     HindIII to PstI inclusive, copy 1"
     misc_feature    2558..5282
                     /label=ACMV DNA-B monomer unit, nick site to nick site
                     /note="ACMV DNA-B monomer unit, nick site to nick site"
     misc_feature    2657..3898
                     /note="unique part of ACMV DNA-B partial tandem dimer, PstI
                     to HindIII, noninclusive"
     gene            2992..3762
                     /gene="BV1"
                     /label=BV1
     CDS             2992..3762
                     /codon_start=1
                     /transl_table=11
                     /gene="BV1"
                     /product="NSP"
                     /label=BV1
                     /protein_id="QNR00651.1"
                     /translation="MYSIRKQSRNLQRKYNSNTTNRYPIRRKYVAGHTRPCVRRRLSYE
                     PVERPLVHNVLCEKQHGDVFNLQQNTSYTSFVTYPARGSSGDGRSRDYIKLQSMSVSGV
                     IHAKADGNDDPMELSQVVNGVFVFSLIMDTKPYLPAGVQALPTFEELFGPYSACYVNLR
                     LLNNQQHRYRVLHSVKRFVSSSGDTKVSQFRFNKRLSTRRYTIWASFHDVDLVNAGGNY
                     RNISKNAILVSYAFVSEHAMSCKPFVQIETSYVG"
     gene            complement(3771..4667)
                     /gene="BC1"
                     /label=BC1
     CDS             complement(3771..4667)
                     /codon_start=1
                     /transl_table=11
                     /gene="BC1"
                     /product="MP"
                     /label=BC1
                     /protein_id="QNR00652.1"
                     /translation="MDTSVPVISSDYIHSARTEYKLTNDESPITLQFPSTLERTRVRIM
                     GKCMKVDHVVIEYRNQVPFNAQGSVIVTIRDTRLSDEQQDQAQFTFPIGCNVDLHYFSA
                     SYFSIDDNVPWQLLYKVEDSNVKNGITFAQIKAKLKLSAAKHSTDIKFKQPTIKILSKD
                     YGPDCVDFWSVGKPKPIRRLIQNEPGTDYDTGPKYRPITVQPGETWATKSTIGRSTSMR
                     YTGPQPIDIDASSSKQYASEAEFPLRGLHRLPEASLDPGDSVSQTQSMSKKDIESIIEQ
                     TVNKCLIAHRGSSHKDL"
     misc_feature    3899..5381
                     /note="duplicated part of ACMV DNA-B partial tandem dimer, 
                     HindIII to PstI inclusive, copy 2"
     primer_bind     complement(5421..5437)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5911..6015
                     /label=AmpR promoter
     CDS             6016..6873
                     /label=AmpR
                     /note="beta-lactamase"
ORIGIN
        1 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
       61 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
      121 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
      181 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
      241 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
      301 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
      361 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
      421 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
      481 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
      541 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
      601 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
      661 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
      721 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
      781 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
      841 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
      901 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
      961 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     1021 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     1081 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     1141 cacacaggaa acagctatga ccatgattac gccaagcttc aggtagccgg tgtagtcctc
     1201 taagtgggaa ttctgcctca gaggcatact gcttggagga tgaggcgtcg atgtcaattg
     1261 gttgtgggcc tgtgtatcgc atgcttgtag agcgcccaat tgtggatttt gtagcccatg
     1321 tttctcctgg ttggaccgtg atgggcctat atttaggccc agtgtcataa tcagtgccgg
     1381 gctcattctg aatgagtctt cgaatgggct taggttttcc aacggaccag aagtcgacac
     1441 agtcaggccc atagtctttt gatagtattt ttatagtggg ctgtttaaat ttaatgtccg
     1501 ttgaatgttt ggcagctgac agcttcagtt ttgccttaat ttgggcaaat gtgataccat
     1561 tttttacgtt tgaatcttca accttataca gcagctgcca tggtacgtta tcatcaattg
     1621 aaaagtatga tgcagagaaa taatggagat ccacattaca ccctatggga aatgtgaatt
     1681 gggcttgatc ctgttgctca tcactgagcc tagtgtcccg aatggtaaca ataacagaac
     1741 cttgtgcatt aaagggcacc tgattcctgt actcaatgac gacatggtct accttcatgc
     1801 atttgcccat gatacgtacc cttgttcgtt caagggtcga aggaaattga agcgtaatcg
     1861 gcgattcatc attcgtcagt ttgtattctg tcctggccga atgaatatag tcgctggaga
     1921 tgacaggtac agatgtatcc atgttcaaca ctcggattat aatcgtgtca aagccacaac
     1981 acctgtctca cgataaacat gatgaattta ttgtaaaact ggccgcgcag cggaatggct
     2041 ttaattcaag ataaagcaga aaattgtggt aacaggagaa agatatttta gaaatataga
     2101 catatatttc gtatcccatc gtaaaaatgc gtctatgtgc aatccaattc aaacatatat
     2161 atatgtatac agcattagtc gaggaaacag tatatattga gagatatgta cctgataata
     2221 acttgaattg gatatcttct ggagtgtaat gtggatagtc gtattatcca aggggtcata
     2281 taaatatgtg ttatatattt attaaggagt tatccaacga gtgatagttt acgttgttag
     2341 atttgcatgt tcaaatctaa aataatcaag tacagtatag tgagagaaag ttagagagag
     2401 acgctctcaa ctggagacac acttgagcat ctcctcctgt taattggaga cattatatag
     2461 ttgtctctaa atggcattct cgtaataagt ggaactttaa tttgaatgaa aaagctcaaa
     2521 aggctcagaa cacccaaggg gccaaccgta taatattacc ggttggcccc gccactcttt
     2581 aaacgtggtc cccgcgcacc ggttggcccc acccccattt gaaaatggtc cccccacctt
     2641 tttcccgcgc ctgcaggtac tatgggggcc ccgcgtgcag gtgttgtctg ctatggaaat
     2701 tgcatgtgtg ccccacatgc aaataaatga tctgcgggcc tatcgaatga cagcttgtgc
     2761 tactaaatta tatttagtat attggtcatt cctcggaata taataaaatg gtatatttta
     2821 tttaactgtt aagagtagcc attcgatatt agtgatttat atgcatcaat ctgacaacga
     2881 ccataaagga caattaaggc tggtagcgta attgtcgatg tattatatat agggtactaa
     2941 catacaggag ttgtgaatag caattatcgg aagtatatac attgatttgt aatgtatagt
     3001 atcaggaagc agtctagaaa tttgcaaagg aaatataaca gcaataccac taacaggtat
     3061 ccaatcagac gtaagtatgt tgctgggcat actagaccat gtgttaggcg aagattgtca
     3121 tatgagccag tggagagacc gttggttcat aatgttttgt gtgagaagca acatggtgat
     3181 gtgtttaatt tgcagcagaa cacaagttac acgtcatttg tgacgtatcc tgccagggga
     3241 tcatctggtg atgggcgtag tcgggattac atcaagttgc aaagtatgtc tgtttcggga
     3301 gtgatacatg cgaaggctga tggcaatgat gatcctatgg agttgtcgca agtggttaat
     3361 ggagtgttcg tgtttagctt aatcatggac acaaagccgt atttacctgc tggtgttcaa
     3421 gctcttccta catttgaaga gctgttcgga ccatattccg cctgttacgt taacttaaga
     3481 ttattgaata atcaacagca tcgatatagg gtattgcaca gcgtgaaacg tttcgtttca
     3541 tcgtcaggag atactaaggt atcgcaattt aggtttaata agcgattgag caccaggcga
     3601 tatacgattt gggcatcatt ccatgatgtg gatctggtaa atgcaggtgg aaattatagg
     3661 aacataagca agaatgctat tctagtcagt tatgcttttg tatcagagca tgcaatgtct
     3721 tgtaagccat ttgtacaaat agaaacatcc tatgttggat gatttattaa tcataaatct
     3781 ttatggctac tgcctctatg tgctattaag cacttgttta cagtttgttc tataatgctt
     3841 tcaatgtcct ttttcgacat ggattgggtt tgtgaaacgg agtcccctgg atctagggaa
     3901 gcttcaggta gccggtgtag tcctctaagt gggaattctg cctcagaggc atactgcttg
     3961 gaggatgagg cgtcgatgtc aattggttgt gggcctgtgt atcgcatgct tgtagagcgc
     4021 ccaattgtgg attttgtagc ccatgtttct cctggttgga ccgtgatggg cctatattta
     4081 ggcccagtgt cataatcagt gccgggctca ttctgaatga gtcttcgaat gggcttaggt
     4141 tttccaacgg accagaagtc gacacagtca ggcccatagt cttttgatag tatttttata
     4201 gtgggctgtt taaatttaat gtccgttgaa tgtttggcag ctgacagctt cagttttgcc
     4261 ttaatttggg caaatgtgat accatttttt acgtttgaat cttcaacctt atacagcagc
     4321 tgccatggta cgttatcatc aattgaaaag tatgatgcag agaaataatg gagatccaca
     4381 ttacacccta tgggaaatgt gaattgggct tgatcctgtt gctcatcact gagcctagtg
     4441 tcccgaatgg taacaataac agaaccttgt gcattaaagg gcacctgatt cctgtactca
     4501 atgacgacat ggtctacctt catgcatttg cccatgatac gtacccttgt tcgttcaagg
     4561 gtcgaaggaa attgaagcgt aatcggcgat tcatcattcg tcagtttgta ttctgtcctg
     4621 gccgaatgaa tatagtcgct ggagatgaca ggtacagatg tatccatgtt caacactcgg
     4681 attataatcg tgtcaaagcc acaacacctg tctcacgata aacatgatga atttattgta
     4741 aaactggccg cgcagcggaa tggctttaat tcaagataaa gcagaaaatt gtggtaacag
     4801 gagaaagata ttttagaaat atagacatat atttcgtatc ccatcgtaaa aatgcgtcta
     4861 tgtgcaatcc aattcaaaca tatatatatg tatacagcat tagtcgagga aacagtatat
     4921 attgagagat atgtacctga taataacttg aattggatat cttctggagt gtaatgtgga
     4981 tagtcgtatt atccaagggg tcatataaat atgtgttata tatttattaa ggagttatcc
     5041 aacgagtgat agtttacgtt gttagatttg catgttcaaa tctaaaataa tcaagtacag
     5101 tatagtgaga gaaagttaga gagagacgct ctcaactgga gacacacttg agcatctcct
     5161 cctgttaatt ggagacatta tatagttgtc tctaaatggc attctcgtaa taagtggaac
     5221 tttaatttga atgaaaaagc tcaaaaggct cagaacaccc aaggggccaa ccgtataata
     5281 ttaccggttg gccccgccac tctttaaacg tggtccccgc gcaccggttg gccccacccc
     5341 catttgaaaa tggtcccccc acctttttcc cgcgcctgca ggtcgactct agaggatccc
     5401 cgggtaccga gctcgaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     5461 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     5521 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
     5581 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
     5641 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     5701 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     5761 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     5821 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     5881 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     5941 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     6001 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     6061 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     6121 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     6181 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     6241 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     6301 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     6361 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     6421 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     6481 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     6541 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     6601 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     6661 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     6721 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     6781 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     6841 gctgagatag gtgcctcact gattaagcat tggtaa
//
