LOCUS       62056_21970        4876 bp DNA     circular SYN 02-APR-2019
DEFINITION  Cloning vector pUC18T-miniTn7T-Apr, complete sequence.
ACCESSION   MK356268
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Ducas-Mowchun K, De Silva PM, Crisostomo L, Fernando DM, Chao TC, 
            Pelka P, Schweizer HP, Kumar A.
  TITLE     Next Generation of Tn7-based single copy insertion elements for use 
            in multi- and pandrug resistant strains of Acinetobacter baumannii
  JOURNAL   Appl. Environ. Microbiol. (2019) In press
  PUBMED    30902859
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   Ducas-Mowchun K, De Silva PM, Kumar A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-JAN-2019) Department of Microbiology, University of 
            Manitoba, 45, Chancellor Circle, Winnipeg, Manitoba R3T2N2, Canada
REFERENCE   3  (bases 1 to 4876)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol. (2019) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-JAN-2019) Department of Microbiology, University of Manitoba, 
            45, Chancellor Circle, Winnipeg, Manitoba R3T2N2, Canada"
FEATURES             Location/Qualifiers
     source          1..4876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  382..580
                     /mobile_element_type="transposon:Tn7R"
                     /label=n7R
                     /note="right end of Tn7"
     primer_bind     585..601
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      629..723
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      826..912
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    958..1005
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(1123..1923)
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     promoter        complement(2164..2192)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     misc_feature    2233..2280
                     /note="FRT; Flp recombinase target site"
     misc_feature    2307..2384
                     /note="MCS; multiple cloning site"
     mobile_element  complement(2393..2558)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     oriT            complement(2717..2825)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     rep_origin      complement(3057..3645)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3819..4676)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4677..4781)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct agaggaccag ccgcgtaacc tggcaaaatc ggttacggtt gagtaataaa
      361 tggatgccct gcgtaagcgg gtgtgggcgg acaataaagt cttaaactga acaaaataga
      421 tctaaactat gacaataaag tcttaaacta gacagaatag ttgtaaactg aaatcagtcc
      481 agttatgctg tgaaaaagca tactggactt ttgttatggc taaagcaaac tcttcatttt
      541 ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg ggttcgaggt cgacggtatc
      601 gataagctag cttaattagc tgagcttgga ctcctgttga tagatccagt aatgacctca
      661 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag
      721 aatccaagct agactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt
      781 ggtgccctta aacgcctggg gtaatgactc tctagcttga ggcatcaaat aaaacgaaag
      841 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
      901 agtaggacaa atccgccgct aggagcttgc ggcccggacg atcgaattgg ggatcttgaa
      961 gttcctattc cgaagttcct attctctaga aagtatagga acttcagagc gcttttgaag
     1021 ctgatgtgct taaaaactta ctcaatggaa ttctagatcg aattggccgc ggtagataat
     1081 tcggcttctc caacgtcatc tcgttctccg ctcatgagct cagccaatcg actggcgagc
     1141 ggcatcgcat tcttcgcatc ccgcctctgg cggatgcagg aagatcaacg gatctcggcc
     1201 cagttgaccc agggctgtcg ccacaatgtc gcgggagcgg atcaaccgag caaaggcatg
     1261 accgactgga ccttccttct gaaggctctt ctccttgagc cacctgtccg ccaaggcaaa
     1321 gcgctcacag cagtggtcat tctcgagata atcgacgcgt accaacttgc catcctgaag
     1381 aatggtgcag tgtctcggca ccccataggg aacctttgcc atcaactcgg caagatgcag
     1441 cgtcgtgttg gcatcgtgtc ccacgccgag gagaagtacc tgcccatcga gttcatggac
     1501 acgggcgacc gggcttgcag gcgagtgagg tggcaggggc aatggatcag agatgatctg
     1561 ctctgcctgt ggccccgctg ccgcaaaggc aaatggatgg gcgctgcgct ttacatttgg
     1621 caggcgccag aatgtgtcag agataactcc aaggtccggt gtaacgggcg acgtggcagg
     1681 atcgaacggc tcgtcgtcca gacctgacca cgagggcatg acgagcgtcc ctcccggacc
     1741 cagcgcagca cgcagggcct cgatcagtcc aagtggccca tcttcgaggg gccggacgct
     1801 acggaaggag ctgtggacca gcagcacacc gccgggggta accccaaggt tgagaagctg
     1861 accgatgagc tcggcttttc gccattcgta ttgcacgaca ttgcactcca ccgctgatga
     1921 catcagtcga tcatagcacg atcaacggca ctgttgcaaa tagtcggtgg tgataaactt
     1981 atcatcccct tttgctgatg gagctgcaca tgaaaagccg aattatccat tgctgttgac
     2041 aaagggaatt gtacaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta
     2101 ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat
     2161 tacagtttac gaaccgaaca ggcttatgtc aattcgatcc attgctgttg acaaagggaa
     2221 tcaggggatc ttgaagttcc tattccgaag ttcctattct ctagaaagta taggaacttc
     2281 agagcgcttt tgaagctaat tcgatcatgc atgagctcac tagtggatcc cccgggctgc
     2341 aggaattcct cgagaagctt gggcccggta cctcgcgaag gccttgcagg ccaaccagat
     2401 aagtgaaatc tagttccaaa ctattttgtc atttttaatt ttcgtattag cttacgacgc
     2461 tacacccagt tcccatctat tttgtcactc ttccctaaat aatccttaaa aactccattt
     2521 ccacccctcc cagttcccaa ctattttgtc cgcccacagc ggggcatttt tcttcctgtt
     2581 atgtttgggc gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     2641 ggcgctcttc cgcttcctcg ctcactgacc cgctgcgctc ggtcgttcgg ctgcggcgag
     2701 cggtatcaga gcttatcggc cagcctcgca gagcaggatt cccgttgagc accgccaggt
     2761 gcgaataagg gacagtgaag aaggaacacc cgctcgcggg tgggcctact tcacctatcc
     2821 tgcccggctg acgccgttgg atacaccaag gaaagtctac acgaaccctt tggcaaaatc
     2881 ctgtatatcg tgcgaaaaag gatggatata ccgaaaaaat cgctataatg accccgaagc
     2941 agggttatgc agcggaaagt ataccttaag gaatcccctg ataacgcagg aaagaacatg
     3001 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     3061 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcgagtca gaggtggcga
     3121 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     3181 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     3241 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     3301 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     3361 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     3421 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     3481 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     3541 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     3601 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     3661 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     3721 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     3781 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     3841 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     3901 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     3961 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     4021 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     4081 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     4141 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     4201 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     4261 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     4321 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     4381 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     4441 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     4501 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     4561 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     4621 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     4681 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     4741 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     4801 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     4861 acgaggccct ttcgtc
//
