LOCUS       62056_21965        5409 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5409
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     oriT            2052..2160
                     /label=oriT
                     /note="incP origin of transfer"
     mobile_element  2319..2484
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     CDS             2664..3356
                     /codon_start=1
                     /label=TurboRFP
                     /note="red fluorescent protein from Entacmaea quadricolor"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     terminator      3379..3473
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      3576..3662
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    3716..3763
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        3874..3921
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             3940..4311
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      complement(4498..4584)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      complement(4687..4781)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(4809..4825)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac tcgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcagg ggattcctta aggtatactt
     1921 tccgctgcat aaccctgctt cggggtcatt atagcgattt tttcggtata tccatccttt
     1981 ttcgcacgat atacaggatt ttgccaaagg gttcgtgtag actttccttg gtgtatccaa
     2041 cggcgtcagc cgggcaggat aggtgaagta ggcccacccg cgagcgggtg ttccttcttc
     2101 actgtccctt attcgcacct ggcggtgctc aacgggaatc ctgctctgcg aggctggccg
     2161 ataagctctg ataccgctcg ccgcagccga acgaccgagc gcagcgggtc agtgagcgag
     2221 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     2281 tgcagcgccc aaacataaca ggaagaaaaa tgccccgctg tgggcggaca aaatagttgg
     2341 gaactgggag gggtggaaat ggagttttta aggattattt agggaagagt gacaaaatag
     2401 atgggaactg ggtgtagcgt cgtaagctaa tacgaaaatt aaaaatgaca aaatagtttg
     2461 gaactagatt tcacttatct ggttggcctg caaggccttc gcgaggtacc gggcccaagc
     2521 ttctcgagga attcctgcag cccgggggat ccactagtga gctcgttgcg cggtcagaaa
     2581 attattttaa atttcctcta gtcaggccgg aataactccc tataatgcga caccactcta
     2641 gaattaaaga ggagaaatta agcatgagcg agctgatcaa ggagaacatg cacatgaagc
     2701 tgtacatgga gggcaccgtg aacaaccacc acttcaagtg cacatccgag ggcgaaggca
     2761 agccctacga gggcacccag accatgaaga tcaaggtggt cgagggcggc cctctcccct
     2821 tcgccttcga catcctggct accagcttca tgtacggcag caaagccttc atcaaccaca
     2881 cccagggcat ccccgacttc tttaagcagt ccttccctga gggcttcaca tgggagagaa
     2941 tcaccacata cgaagacggg ggcgtgctga ccgctaccca ggacaccagc ttccagaacg
     3001 gctgcatcat ctacaacgtc aagatcaacg gggtgaactt cccatccaac ggccctgtga
     3061 tgcagaagaa aacacgcggc tgggaggcca acaccgagat gctgtacccc gctgacggcg
     3121 gcctgagagg ccacagccag atggccctga agctcgtggg cgggggctac ctgcactgct
     3181 ccttcaagac cacatacaga tccaagaaac ccgctaagaa cctcaagatg cccggcttcc
     3241 acttcgtgga ccacagactg gaaagaatca aggaggccga caaagagacc tacgtcgagc
     3301 agcacgagat ggctgtggcc aagtactgcg acctccctag caaactgggg cacagataag
     3361 cttaattagc tgagcttgga ctcctgttga tagatccagt aatgacctca gaactccatc
     3421 tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag aatccaagct
     3481 agactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta
     3541 aacgcctggg gtaatgactc tctagcttga ggcatcaaat aaaacgaaag gctcagtcga
     3601 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa
     3661 atccgccgct aggagcttgc ggcccgagct catgcatgat cgaattgggg atcttgaagt
     3721 acctattccg aagttcctat tctctagaaa gtataggaac ttcagagcgc ttttgaagct
     3781 gatgtgctta aaaacttact caatggaata attctagata ttcggcttcg acgcgtaact
     3841 cacgttaagg gattttggtc atgatcagca cgtgttgaca attaatcatc ggcatagtat
     3901 atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc
     3961 gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc
     4021 gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc
     4081 ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg
     4141 gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg
     4201 gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc
     4261 ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgatcatga
     4321 tgatatattt ttatcttgtg caatgtaaca tcagagataa atccattgct gttgacaaag
     4381 ggaatcaggg gatcttgaag ttcctattcc gaagttccta ttctctagaa agtataggaa
     4441 cttcagagcg cttttgaagc taattcgatc gtccgggccg caagctccta gcggcggatt
     4501 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttt
     4561 cgactgagcc tttcgtttta tttgatgcct caagctagag agtcattacc ccaggcgttt
     4621 aagggcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca tcgcagtcta
     4681 gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag
     4741 atggagttct gaggtcatta ctggatctat caacaggagt ccaagctcag ctaattaagc
     4801 tagcttatcg ataccgtcga cctcgaaccc cacgcccctc tttaatacga cgggcaattt
     4861 gcacttcaga aaatgaagag tttgctttag ccataacaaa agtccagtat gctttttcac
     4921 agcataactg gactgatttc agtttacaac tattctgtct agtttaagac tttattgtca
     4981 tagtttagat ctattttgtt cagtttaaga ctttattgtc cgcccacacc cgcttacgca
     5041 gggcatccat ttattactca accgtaaccg attttgccag gttacgcggc tggtcctcta
     5101 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     5161 atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     5221 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     5281 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     5341 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     5401 aacgcgcga
//
