LOCUS       V015889                 6225 bp    DNA     circular SYN 02-APR-2019
DEFINITION  Exported.
ACCESSION   V015889
VERSION     V015889
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Ducas-Mowchun K, De Silva PM, Crisostomo L, Fernando DM, Chao TC,
            Pelka P, Schweizer HP, Kumar A.
  TITLE     Next Generation of Tn7-based single copy insertion elements for use
            in multi- and pandrug resistant strains of Acinetobacter baumannii
  JOURNAL   Appl. Environ. Microbiol. (2019) In press
   PUBMED   30902859
REFERENCE   2  (bases 1 to 6225)
  AUTHORS   Ducas-Mowchun K, De Silva PM, Kumar A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-NOV-2018) Department of Microbiology, University of
            Manitoba, 45, Chancellor Circle, Winnipeg, Manitoba R3T2N2, Canada
REFERENCE   3  (bases 1 to 6225)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol. (2019) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-NOV-2018) Department of Microbiology, University of Manitoba,
            45, Chancellor Circle, Winnipeg, Manitoba R3T2N2, Canada"
FEATURES             Location/Qualifiers
     source          1..6225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  382..580
                     /mobile_element_type="transposon:Tn7R"
                     /label="n7R"
                     /note="right end of Tn7"
     primer_bind     585..601
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     terminator      629..723
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     terminator      826..912
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    958..1005
                     /label="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(1123..1923)
                     /label="ApmR"
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     promoter        complement(2164..2192)
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     misc_feature    2233..2280
                     /note="FRT; Flp recombinase target site"
     misc_feature    2307..2355
                     /note="MCS; multiple cloning site"
     CDS             complement(2364..3080)
                     /label="mTurquoise2"
                     /note="enhanced monomeric variant of CFP (Goedhart et al.,
                     2012)"
     CDS             complement(3392..3505)
                     /gene="rpmJ"
                     /label="Large ribosomal subunit protein bL36A"
                     /note="Large ribosomal subunit protein bL36A from
                     Escherichia coli (strain K12). Accession#: P0A7Q6"
     misc_feature    3721..3733
                     /note="MCS; multiple cloning site"
     mobile_element  complement(3742..3907)
                     /label="Tn7L"
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     oriT            complement(4066..4174)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     rep_origin      complement(4406..4994)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5168..6025)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6026..6130)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct agaggaccag ccgcgtaacc tggcaaaatc ggttacggtt gagtaataaa
      361 tggatgccct gcgtaagcgg gtgtgggcgg acaataaagt cttaaactga acaaaataga
      421 tctaaactat gacaataaag tcttaaacta gacagaatag ttgtaaactg aaatcagtcc
      481 agttatgctg tgaaaaagca tactggactt ttgttatggc taaagcaaac tcttcatttt
      541 ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg ggttcgaggt cgacggtatc
      601 gataagctag cttaattagc tgagcttgga ctcctgttga tagatccagt aatgacctca
      661 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag
      721 aatccaagct agactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt
      781 ggtgccctta aacgcctggg gtaatgactc tctagcttga ggcatcaaat aaaacgaaag
      841 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
      901 agtaggacaa atccgccgct aggagcttgc ggcccggacg atcgaattgg ggatcttgaa
      961 gttcctattc cgaagttcct attctctaga aagtatagga acttcagagc gcttttgaag
     1021 ctgatgtgct taaaaactta ctcaatggaa ttctagatcg aattggccgc ggtagataat
     1081 tcggcttctc caacgtcatc tcgttctccg ctcatgagct cagccaatcg actggcgagc
     1141 ggcatcgcat tcttcgcatc ccgcctctgg cggatgcagg aagatcaacg gatctcggcc
     1201 cagttgaccc agggctgtcg ccacaatgtc gcgggagcgg atcaaccgag caaaggcatg
     1261 accgactgga ccttccttct gaaggctctt ctccttgagc cacctgtccg ccaaggcaaa
     1321 gcgctcacag cagtggtcat tctcgagata atcgacgcgt accaacttgc catcctgaag
     1381 aatggtgcag tgtctcggca ccccataggg aacctttgcc atcaactcgg caagatgcag
     1441 cgtcgtgttg gcatcgtgtc ccacgccgag gagaagtacc tgcccatcga gttcatggac
     1501 acgggcgacc gggcttgcag gcgagtgagg tggcaggggc aatggatcag agatgatctg
     1561 ctctgcctgt ggccccgctg ccgcaaaggc aaatggatgg gcgctgcgct ttacatttgg
     1621 caggcgccag aatgtgtcag agataactcc aaggtccggt gtaacgggcg acgtggcagg
     1681 atcgaacggc tcgtcgtcca gacctgacca cgagggcatg acgagcgtcc ctcccggacc
     1741 cagcgcagca cgcagggcct cgatcagtcc aagtggccca tcttcgaggg gccggacgct
     1801 acggaaggag ctgtggacca gcagcacacc gccgggggta accccaaggt tgagaagctg
     1861 accgatgagc tcggcttttc gccattcgta ttgcacgaca ttgcactcca ccgctgatga
     1921 catcagtcga tcatagcacg atcaacggca ctgttgcaaa tagtcggtgg tgataaactt
     1981 atcatcccct tttgctgatg gagctgcaca tgaaaagccg aattatccat tgctgttgac
     2041 aaagggaatt gtacaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta
     2101 ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat
     2161 tacagtttac gaaccgaaca ggcttatgtc aattcgatcc attgctgttg acaaagggaa
     2221 tcaggggatc ttgaagttcc tattccgaag ttcctattct ctagaaagta taggaacttc
     2281 agagcgcttt tgaagctaat tcgatcatgc atgagctcac tagtggatcc cccgggctgc
     2341 aggaattcct cgagaagctt ttacttgtac agctcgtcca tgccgagagt gatcccggcg
     2401 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagc
     2461 ttggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg
     2521 gtgttctgct ggtagtggtc ggcgagctgc acgccgccgt cctcgatgtt gtggcggatc
     2581 ttgaagttgg ccttgatgcc gttcttctgc ttgtcggcgg tgatatagac gttgtcgcta
     2641 aagtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc
     2701 ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg
     2761 tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg
     2821 gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc gggcgaagca ctgcacgccc
     2881 caggacaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg
     2941 aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac
     3001 ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc
     3061 tcctcgccct tgctcaccat atgtatatct ccttcttaaa tctagaggat cttaacattt
     3121 tcagcgatac ccgctgcagc caggatggct ttagaacggg tcttgccgac accgtagatc
     3181 gaagttaacg cgatcacggc gtgtttctga tcaggaatgt taatgcctgc tatacgggcc
     3241 actatgcact cctactattt aatatgtacg taccatgctg aaaagcccgt tttcaggata
     3301 ctcaaatgga aacgtacaga catacaaaag attggctggc taatctagcc agctcaaccc
     3361 aactttgcaa gaaaaatatg cgaaaaaatc agccttggcg ctgtttatgc ttcggctcgg
     3421 cactgcaaat cacacggatg acaccatcac gcttaacgat tttgcagtta cggcataatt
     3481 tcttgacgga agcacgaact ttcattttta ctctccgtaa cttctcaggc gaccaattaa
     3541 cggccgtagc ctttcaggtt cgccttcttc aatgcagact catactgact ggacatcatc
     3601 agagtttgca cttgagccat aaagtccata atcacgacaa caacgataag cagtgaggtc
     3661 ccaccgaagt agaacggcac tttcattgca tcacgcatga actccgggat ccccgggtac
     3721 ctcgcgaagg ccttgcaggc caaccagata agtgaaatct agttccaaac tattttgtca
     3781 tttttaattt tcgtattagc ttacgacgct acacccagtt cccatctatt ttgtcactct
     3841 tccctaaata atccttaaaa actccatttc cacccctccc agttcccaac tattttgtcc
     3901 gcccacagcg gggcattttt cttcctgtta tgtttgggcg ctgcattaat gaatcggcca
     3961 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgaccc
     4021 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagag cttatcggcc agcctcgcag
     4081 agcaggattc ccgttgagca ccgccaggtg cgaataaggg acagtgaaga aggaacaccc
     4141 gctcgcgggt gggcctactt cacctatcct gcccggctga cgccgttgga tacaccaagg
     4201 aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagg atggatatac
     4261 cgaaaaaatc gctataatga ccccgaagca gggttatgca gcggaaagta taccttaagg
     4321 aatcccctga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     4381 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4441 aaaatcgacg ctcgagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     4501 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     4561 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     4621 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     4681 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     4741 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     4801 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     4861 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     4921 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     4981 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     5041 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     5101 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     5161 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     5221 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     5281 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     5341 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     5401 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     5461 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     5521 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     5581 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     5641 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     5701 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     5761 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     5821 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     5881 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     5941 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     6001 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     6061 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     6121 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     6181 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc
//
