LOCUS       V015964                 6222 bp    DNA     circular SYN 02-APR-2019
DEFINITION  Exported.
ACCESSION   V015964
VERSION     V015964
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6222)
  AUTHORS   Ducas-Mowchun K, De Silva PM, Crisostomo L, Fernando DM, Chao TC,
            Pelka P, Schweizer HP, Kumar A.
  TITLE     Next Generation of Tn7-based single copy insertion elements for use
            in multi- and pandrug resistant strains of Acinetobacter baumannii
  JOURNAL   Appl. Environ. Microbiol. (2019) In press
   PUBMED   30902859
REFERENCE   2  (bases 1 to 6222)
  AUTHORS   Ducas-Mowchun K, De Silva PM, Kumar A.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-SEP-2018) Department of Microbiology, University of
            Manitoba, 45, Chancellor Circle, Winnipeg, Manitoba R3T2N2, Canada
REFERENCE   3  (bases 1 to 6222)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol. (2019) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (24-SEP-2018) Department of Microbiology, University of Manitoba,
            45, Chancellor Circle, Winnipeg, Manitoba R3T2N2, Canada"
FEATURES             Location/Qualifiers
     source          1..6222
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  382..580
                     /mobile_element_type="transposon:Tn7R"
                     /label="n7R"
                     /note="right end of Tn7"
     primer_bind     585..601
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     terminator      629..723
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     terminator      826..912
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    958..1005
                     /label="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(1123..1923)
                     /label="ApmR"
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     promoter        complement(2164..2192)
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     misc_feature    2233..2280
                     /note="FRT; Flp recombinase target site"
     misc_feature    2307..2355
                     /note="MCS; multiple cloning site"
     CDS             complement(2370..3077)
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     CDS             complement(3389..3502)
                     /gene="rpmJ"
                     /label="Large ribosomal subunit protein bL36A"
                     /note="Large ribosomal subunit protein bL36A from
                     Escherichia coli (strain K12). Accession#: P0A7Q6"
     misc_feature    3718..3730
                     /note="MCS; multiple cloning site"
     mobile_element  complement(3739..3904)
                     /label="Tn7L"
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     oriT            complement(4063..4171)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     rep_origin      complement(4403..4991)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5165..6022)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6023..6127)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct agaggaccag ccgcgtaacc tggcaaaatc ggttacggtt gagtaataaa
      361 tggatgccct gcgtaagcgg gtgtgggcgg acaataaagt cttaaactga acaaaataga
      421 tctaaactat gacaataaag tcttaaacta gacagaatag ttgtaaactg aaatcagtcc
      481 agttatgctg tgaaaaagca tactggactt ttgttatggc taaagcaaac tcttcatttt
      541 ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg ggttcgaggt cgacggtatc
      601 gataagctag cttaattagc tgagcttgga ctcctgttga tagatccagt aatgacctca
      661 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag
      721 aatccaagct agactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt
      781 ggtgccctta aacgcctggg gtaatgactc tctagcttga ggcatcaaat aaaacgaaag
      841 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
      901 agtaggacaa atccgccgct aggagcttgc ggcccggacg atcgaattgg ggatcttgaa
      961 gttcctattc cgaagttcct attctctaga aagtatagga acttcagagc gcttttgaag
     1021 ctgatgtgct taaaaactta ctcaatggaa ttctagatcg aattggccgc ggtagataat
     1081 tcggcttctc caacgtcatc tcgttctccg ctcatgagct cagccaatcg actggcgagc
     1141 ggcatcgcat tcttcgcatc ccgcctctgg cggatgcagg aagatcaacg gatctcggcc
     1201 cagttgaccc agggctgtcg ccacaatgtc gcgggagcgg atcaaccgag caaaggcatg
     1261 accgactgga ccttccttct gaaggctctt ctccttgagc cacctgtccg ccaaggcaaa
     1321 gcgctcacag cagtggtcat tctcgagata atcgacgcgt accaacttgc catcctgaag
     1381 aatggtgcag tgtctcggca ccccataggg aacctttgcc atcaactcgg caagatgcag
     1441 cgtcgtgttg gcatcgtgtc ccacgccgag gagaagtacc tgcccatcga gttcatggac
     1501 acgggcgacc gggcttgcag gcgagtgagg tggcaggggc aatggatcag agatgatctg
     1561 ctctgcctgt ggccccgctg ccgcaaaggc aaatggatgg gcgctgcgct ttacatttgg
     1621 caggcgccag aatgtgtcag agataactcc aaggtccggt gtaacgggcg acgtggcagg
     1681 atcgaacggc tcgtcgtcca gacctgacca cgagggcatg acgagcgtcc ctcccggacc
     1741 cagcgcagca cgcagggcct cgatcagtcc aagtggccca tcttcgaggg gccggacgct
     1801 acggaaggag ctgtggacca gcagcacacc gccgggggta accccaaggt tgagaagctg
     1861 accgatgagc tcggcttttc gccattcgta ttgcacgaca ttgcactcca ccgctgatga
     1921 catcagtcga tcatagcacg atcaacggca ctgttgcaaa tagtcggtgg tgataaactt
     1981 atcatcccct tttgctgatg gagctgcaca tgaaaagccg aattatccat tgctgttgac
     2041 aaagggaatt gtacaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta
     2101 ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat
     2161 tacagtttac gaaccgaaca ggcttatgtc aattcgatcc attgctgttg acaaagggaa
     2221 tcaggggatc ttgaagttcc tattccgaag ttcctattct ctagaaagta taggaacttc
     2281 agagcgcttt tgaagctaat tcgatcatgc atgagctcac tagtggatcc cccgggctgc
     2341 aggaattcct cgagaagctt gcatgcttac ttgtacagct cgtccatgcc gccggtggag
     2401 tggcggccct cggcgcgttc gtactgttcc acgatggtgt agtcctcgtt gtgggaggtg
     2461 atgtccaact tgatgttgac gttgtaggcg ccgggcagct gcacgggctt cttggccttg
     2521 taggtggtct tgacctcagc gtcgtagtgg ccgccgtcct tcagcttcag cctctgcttg
     2581 atctcgccct tcagggcgcc gtcctcgggg tacatccgct cggaggaggc ctcccagccc
     2641 atggtcttct tctgcattac ggggccgtcg gaggggaagt tggtgccgcg cagcttcacc
     2701 ttgtagatga actcgccgtc ctgcagggag gagtcctggg tcacggtcac cacgccgccg
     2761 tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg ggaaggacag cttcaagtag
     2821 tcggggatgt cggcggggtg cttcacgtag gccttggagc cgtacatgaa ctgaggggac
     2881 aggatgtccc aggcgaaggg cagggggcca cccttggtca ccttcagctt ggcggtctgg
     2941 gtgccctcgt aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg gccgttcacg
     3001 gagccctcca tgtgcacctt gaagcgcatg aactccttga tgatggccat gttatcctcc
     3061 tcgcccttgc tcaccatatg tatatctcct tcttaaatct agaggatctt aacattttca
     3121 gcgatacccg ctgcagccag gatggcttta gaacgggtct tgccgacacc gtagatcgaa
     3181 gttaacgcga tcacggcgtg tttctgatca ggaatgttaa tgcctgctat acgggccact
     3241 atgcactcct actatttaat atgtacgtac catgctgaaa agcccgtttt caggatactc
     3301 aaatggaaac gtacagacat acaaaagatt ggctggctaa tctagccagc tcaacccaac
     3361 tttgcaagaa aaatatgcga aaaaatcagc cttggcgctg tttatgcttc ggctcggcac
     3421 tgcaaatcac acggatgaca ccatcacgct taacgatttt gcagttacgg cataatttct
     3481 tgacggaagc acgaactttc atttttactc tccgtaactt ctcaggcgac caattaacgg
     3541 ccgtagcctt tcaggttcgc cttcttcaat gcagactcat actgactgga catcatcaga
     3601 gtttgcactt gagccataaa gtccataatc acgacaacaa cgataagcag tgaggtccca
     3661 ccgaagtaga acggcacttt cattgcatca cgcatgaact ccgggatccc cgggtacctc
     3721 gcgaaggcct tgcaggccaa ccagataagt gaaatctagt tccaaactat tttgtcattt
     3781 ttaattttcg tattagctta cgacgctaca cccagttccc atctattttg tcactcttcc
     3841 ctaaataatc cttaaaaact ccatttccac ccctcccagt tcccaactat tttgtccgcc
     3901 cacagcgggg catttttctt cctgttatgt ttgggcgctg cattaatgaa tcggccaacg
     3961 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgacccgct
     4021 gcgctcggtc gttcggctgc ggcgagcggt atcagagctt atcggccagc ctcgcagagc
     4081 aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct
     4141 cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa
     4201 gtctacacga accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga
     4261 aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg gaaagtatac cttaaggaat
     4321 cccctgataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     4381 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     4441 atcgacgctc gagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     4501 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     4561 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     4621 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     4681 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     4741 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     4801 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     4861 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     4921 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     4981 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     5041 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     5101 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     5161 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     5221 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     5281 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     5341 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     5401 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     5461 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     5521 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     5581 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     5641 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     5701 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     5761 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     5821 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     5881 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     5941 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     6001 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     6061 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     6121 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     6181 cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//
