LOCUS       V016100                 6219 bp    DNA     circular SYN 02-APR-2019
DEFINITION  Exported.
ACCESSION   V016100
VERSION     V016100
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6219)
  AUTHORS   Ducas-Mowchun K, De Silva PM, Crisostomo L, Fernando DM, Chao TC,
            Pelka P, Schweizer HP, Kumar A.
  TITLE     Next Generation of Tn7-based single copy insertion elements for use
            in multi- and pandrug resistant strains of Acinetobacter baumannii
  JOURNAL   Appl. Environ. Microbiol. (2019) In press
   PUBMED   30902859
REFERENCE   2  (bases 1 to 6219)
  AUTHORS   Ducas-Mowchun K, De Silva PM, Kumar A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-NOV-2018) Department of Microbiology, University of
            Manitoba, 45, Chancellor Circle, Winnipeg, Manitoba R3T2N2, Canada
REFERENCE   3  (bases 1 to 6219)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
            Environ. Microbiol. (2019) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (20-NOV-2018) Department of Microbiology, University of Manitoba,
            45, Chancellor Circle, Winnipeg, Manitoba R3T2N2, Canada"
FEATURES             Location/Qualifiers
     source          1..6219
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  382..580
                     /mobile_element_type="transposon:Tn7R"
                     /label="n7R"
                     /note="right end of Tn7"
     primer_bind     585..601
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     terminator      629..723
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     terminator      826..912
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    958..1005
                     /label="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(1123..1923)
                     /label="ApmR"
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     promoter        complement(2164..2192)
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     misc_feature    2233..2280
                     /note="FRT; Flp recombinase target site"
     misc_feature    2307..2355
                     /note="MCS; multiple cloning site"
     CDS             complement(2364..3074)
                     /label="mRuby2"
                     /note="monomeric red fluorescent protein derived from
                     mRuby, with improved photophysical properties (Lam et al.,
                     2012)"
     CDS             complement(3386..3499)
                     /gene="rpmJ"
                     /label="Large ribosomal subunit protein bL36A"
                     /note="Large ribosomal subunit protein bL36A from
                     Escherichia coli (strain K12). Accession#: P0A7Q6"
     misc_feature    3715..3727
                     /note="MCS; multiple cloning site"
     mobile_element  complement(3736..3901)
                     /label="Tn7L"
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     oriT            complement(4060..4168)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     rep_origin      complement(4400..4988)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5162..6019)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6020..6124)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct agaggaccag ccgcgtaacc tggcaaaatc ggttacggtt gagtaataaa
      361 tggatgccct gcgtaagcgg gtgtgggcgg acaataaagt cttaaactga acaaaataga
      421 tctaaactat gacaataaag tcttaaacta gacagaatag ttgtaaactg aaatcagtcc
      481 agttatgctg tgaaaaagca tactggactt ttgttatggc taaagcaaac tcttcatttt
      541 ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg ggttcgaggt cgacggtatc
      601 gataagctag cttaattagc tgagcttgga ctcctgttga tagatccagt aatgacctca
      661 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag
      721 aatccaagct agactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt
      781 ggtgccctta aacgcctggg gtaatgactc tctagcttga ggcatcaaat aaaacgaaag
      841 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
      901 agtaggacaa atccgccgct aggagcttgc ggcccggacg atcgaattgg ggatcttgaa
      961 gttcctattc cgaagttcct attctctaga aagtatagga acttcagagc gcttttgaag
     1021 ctgatgtgct taaaaactta ctcaatggaa ttctagatcg aattggccgc ggtagataat
     1081 tcggcttctc caacgtcatc tcgttctccg ctcatgagct cagccaatcg actggcgagc
     1141 ggcatcgcat tcttcgcatc ccgcctctgg cggatgcagg aagatcaacg gatctcggcc
     1201 cagttgaccc agggctgtcg ccacaatgtc gcgggagcgg atcaaccgag caaaggcatg
     1261 accgactgga ccttccttct gaaggctctt ctccttgagc cacctgtccg ccaaggcaaa
     1321 gcgctcacag cagtggtcat tctcgagata atcgacgcgt accaacttgc catcctgaag
     1381 aatggtgcag tgtctcggca ccccataggg aacctttgcc atcaactcgg caagatgcag
     1441 cgtcgtgttg gcatcgtgtc ccacgccgag gagaagtacc tgcccatcga gttcatggac
     1501 acgggcgacc gggcttgcag gcgagtgagg tggcaggggc aatggatcag agatgatctg
     1561 ctctgcctgt ggccccgctg ccgcaaaggc aaatggatgg gcgctgcgct ttacatttgg
     1621 caggcgccag aatgtgtcag agataactcc aaggtccggt gtaacgggcg acgtggcagg
     1681 atcgaacggc tcgtcgtcca gacctgacca cgagggcatg acgagcgtcc ctcccggacc
     1741 cagcgcagca cgcagggcct cgatcagtcc aagtggccca tcttcgaggg gccggacgct
     1801 acggaaggag ctgtggacca gcagcacacc gccgggggta accccaaggt tgagaagctg
     1861 accgatgagc tcggcttttc gccattcgta ttgcacgaca ttgcactcca ccgctgatga
     1921 catcagtcga tcatagcacg atcaacggca ctgttgcaaa tagtcggtgg tgataaactt
     1981 atcatcccct tttgctgatg gagctgcaca tgaaaagccg aattatccat tgctgttgac
     2041 aaagggaatt gtacaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta
     2101 ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat
     2161 tacagtttac gaaccgaaca ggcttatgtc aattcgatcc attgctgttg acaaagggaa
     2221 tcaggggatc ttgaagttcc tattccgaag ttcctattct ctagaaagta taggaacttc
     2281 agagcgcttt tgaagctaat tcgatcatgc atgagctcac tagtggatcc cccgggctgc
     2341 aggaattcct cgagaagctt ttacttgtac agctcgtcca tcccaccacc aagcccggcg
     2401 aacttggcaa ctgcgtgttc gcgttgtact acgaacattt cattgtcact ttcctctaac
     2461 ctttccaggc ggtgatcaac ggcatggata ccgggcatct tgatgttccc gacggtcttt
     2521 tttgacctgt aagttgttac gaaagagcaa gacagatggc caccaccatc aactttcagt
     2581 gccatatgag tgtatcccct cagaccacca tctgctggat acatcatctc tgtattaggc
     2641 tcccaaccct tggtcttctt ctgcatcacg ggaccattgg agggaaagtt tacccctctg
     2701 acttggacgt ggtaaacgag acagccatcc tcaaggctgg tgtcctgcat gacggtgacg
     2761 actccaccat cttcgtatct cgtaactctt tcccaagtaa aaccctcagg aaaggactgt
     2821 ttaaagaaat caggaatgcc tttcgggtac ttgataaaag tacggctgcc atacatgaac
     2881 gacgtggcaa gaatgtcaaa ggcaaatggc aggggtcctc cctcgatgac tttgatcctc
     2941 atggtttgag ttcccatgta cggattgcct tctccttcac ctgtgcattt gaattggtgg
     3001 ccgttgaccg aaccttccat gaccaccttc atacgcatat tttccttgat cagctcttcg
     3061 cccttagaca ccatatgtat atctccttct taaatctaga ggatcttaac attttcagcg
     3121 atacccgctg cagccaggat ggctttagaa cgggtcttgc cgacaccgta gatcgaagtt
     3181 aacgcgatca cggcgtgttt ctgatcagga atgttaatgc ctgctatacg ggccactatg
     3241 cactcctact atttaatatg tacgtaccat gctgaaaagc ccgttttcag gatactcaaa
     3301 tggaaacgta cagacataca aaagattggc tggctaatct agccagctca acccaacttt
     3361 gcaagaaaaa tatgcgaaaa aatcagcctt ggcgctgttt atgcttcggc tcggcactgc
     3421 aaatcacacg gatgacacca tcacgcttaa cgattttgca gttacggcat aatttcttga
     3481 cggaagcacg aactttcatt tttactctcc gtaacttctc aggcgaccaa ttaacggccg
     3541 tagcctttca ggttcgcctt cttcaatgca gactcatact gactggacat catcagagtt
     3601 tgcacttgag ccataaagtc cataatcacg acaacaacga taagcagtga ggtcccaccg
     3661 aagtagaacg gcactttcat tgcatcacgc atgaactccg ggatccccgg gtacctcgcg
     3721 aaggccttgc aggccaacca gataagtgaa atctagttcc aaactatttt gtcattttta
     3781 attttcgtat tagcttacga cgctacaccc agttcccatc tattttgtca ctcttcccta
     3841 aataatcctt aaaaactcca tttccacccc tcccagttcc caactatttt gtccgcccac
     3901 agcggggcat ttttcttcct gttatgtttg ggcgctgcat taatgaatcg gccaacgcgc
     3961 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg acccgctgcg
     4021 ctcggtcgtt cggctgcggc gagcggtatc agagcttatc ggccagcctc gcagagcagg
     4081 attcccgttg agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc
     4141 gggtgggcct acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc
     4201 tacacgaacc ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa
     4261 aatcgctata atgaccccga agcagggtta tgcagcggaa agtatacctt aaggaatccc
     4321 ctgataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     4381 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     4441 gacgctcgag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     4501 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     4561 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     4621 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     4681 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     4741 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     4801 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     4861 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     4921 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     4981 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     5041 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     5101 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     5161 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     5221 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     5281 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     5341 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     5401 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     5461 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     5521 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     5581 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     5641 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     5701 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     5761 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     5821 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     5881 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     5941 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     6001 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     6061 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     6121 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     6181 taacctataa aaataggcgt atcacgaggc cctttcgtc
//
