LOCUS       Exported               11300 bp DNA     circular SYN 27-NOV-2025
DEFINITION  mini-Tn7 luxCDABE transcriptional fusion vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11300)
  AUTHORS   Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer 
            RR, Schweizer HP
  TITLE     A Tn7-based broad-range bacterial cloning and expression system.
  JOURNAL   Nat Methods. 2005 Jun;2(6):443-8.
  PUBMED    15908923
REFERENCE   2  (bases 1 to 11300)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 11300)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11300)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat
            Methods."; date: "2005-06"; volume: "2(6)"; pages: "443-8"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     primer_bind     584..600
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     585..601
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      629..723
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      826..912
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    958..1005
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(1138..1668)
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(1857..1885)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    1926..1973
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             2517..3956
                     /codon_start=1
                     /label=LuxC
                     /note="LuxC fatty acid reductase"
                     /translation="MTKKISFIINGQVEIFPESDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MRHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             3972..4892
                     /codon_start=1
                     /label=LuxD
                     /note="LuxD acyltransferase"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PDNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             4944..6023
                     /codon_start=1
                     /label=LuxA
                     /note="LuxA luciferase subunit"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             6041..7021
                     /codon_start=1
                     /label=LuxB
                     /note="LuxB luciferase subunit"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             7203..8312
                     /codon_start=1
                     /label=LuxE
                     /note="LuxE"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     mobile_element  complement(9046..9211)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     primer_bind     complement(9310..9327)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9481..10069)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10243..11100)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11101..11205)
                     /label=AmpR promoter
     primer_bind     11273..11291
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtta
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct agaggaccag ccgcgtaacc tggcaaaatc ggttacggtt gagtaataaa
      361 tggatgccct gcgtaagcgg gtgtgggcgg acaataaagt cttaaactga acaaaataga
      421 tctaaactat gacaataaag tcttaaacta gacagaatag ttgtaaactg aaatcagtcc
      481 agttatgctg tgaaaaagca tactggactt ttgttatggc taaagcaaac tcttcatttt
      541 ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg ggttcgaggt cgacggtatc
      601 gataagctag cttaattagc tgagcttgga ctcctgttga tagatccagt aatgacctca
      661 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag
      721 aatccaagct agactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt
      781 ggtgccctta aacgcctggg gtaatgactc tctagcttga ggcatcaaat aaaacgaaag
      841 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
      901 agtaggacaa atccgccgct aggagcttgc ggcccggacg atcgaattgg ggatcttgaa
      961 gtacctattc cgaagttcct attctctaga aagtatagga acttcagagc gcttttgaag
     1021 ctgatgtgct taaaaactta ctcaatggaa ttctagatcg aattggccgc ggcgttgtga
     1081 caatttaccg aacaactccg cggccgggaa gccgatctcg gcttgaacga attgttaggt
     1141 ggcggtactt gggtcgatat caaagtgcat cacttcttcc cgtatgccca actttgtata
     1201 gagagccact gcgggatcgt caccgtaatc tgcttgcacg tagatcacat aagcaccaag
     1261 cgcgttggcc tcatgcttga ggagattgat gagcgcggtg gcaatgccct gcctccggtg
     1321 ctcgccggag actgcgagat catagatata gatctcacta cgcggctgct caaacttggg
     1381 cagaacgtaa gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag caagcgcgat
     1441 gaatgtctta ctacggagca agttcccgag gtaatcggag tccggctgat gttgggagta
     1501 ggtggctacg tctccgaact cacgaccgaa aagatcaaga gcagcccgca tggatttgac
     1561 ttggtcaggg ccgagcctac atgtgcgaat gatgcccata cttgagccac ctaactttgt
     1621 tttagggcga ctgccctgct gcgtaacatc gttgctgctg cgtaacatcg ttgctgctcc
     1681 ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag
     1741 actgtacaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc
     1801 tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagtt
     1861 tacgaaccga acaggcttat gtcaattcga tccattgctg ttgacaaagg gaatcagggg
     1921 atcttgaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttcagagcgc
     1981 ttttgaagct aattcgatca tgcatgagct cactagtgga tcccccgggc tgcaggaatt
     2041 cctcgagaag ctgatcccca attcttcttt agaaatctgc cggtaaaaat tagattgcta
     2101 ttcaatctat ttctatcggt atttgtgaaa taatactcag gataataatt tacataaata
     2161 ttatcacgca ttagagaaga gcatgacttt tttaatttaa acttttcatt aacaaatctt
     2221 gttgatatga aaattttcct ttgctatttt aacagatatt aaaacgggaa taggcgttat
     2281 attgacgatc cattcagtta gattaaaaac cttgagcaga aaatttatat tattatcata
     2341 attatgacga aagttacagg ccaggaacca cgtagtcaga atctgatttt ctatatattt
     2401 gttatttaca tcgtcataac acaaaaatat aagaagcaag tgttggtacg accagttcgc
     2461 aagatagtta aacagcaact taagttgaaa ttacccccat taaatggatg gcaaatatga
     2521 ctaaaaaaat ttcattcatt attaacggcc aggttgaaat ctttcccgaa agtgatgatt
     2581 tagtgcaatc cattaatttt ggtgataata gtgtttacct gccaatattg aatgactctc
     2641 atgtaaaaaa cattattgat tgtaatggaa ataacgaatt acggttgcat aacattgtca
     2701 attttctcta tacggtaggg caaagatgga aaaatgaaga atactcaaga cgcaggacat
     2761 acattcgtga cttaaaaaaa tatatgggat attcagaaga aatggctaag ctagaggcca
     2821 attggatatc tatgatttta tgttctaaag gcggccttta tgatgttgta gaaaatgaac
     2881 ttggttctcg ccatatcatg gatgaatggc tacctcagga tgaaagttat gttcgggctt
     2941 ttccgaaagg taaatctgta catctgttgg caggtaatgt tccattatct gggatcatgt
     3001 ctatattacg cgcaatttta actaagaatc agtgtattat aaaaacatcg tcaaccgatc
     3061 cttttaccgc taatgcatta gcgttaagtt ttattgatgt agaccctaat catccgataa
     3121 cgcgctcttt atctgttata tattggcccc accaaggtga tacatcactc gcaaaagaaa
     3181 ttatgcgaca tgcggatgtt attgtcgctt ggggagggcc agatgcgatt aattgggcgg
     3241 tagagcacgc gccatcttat gctgatgtga ttaaatttgg ttctaaaaag agtctttgca
     3301 ttatcgataa tcctgttgat ttgacgtccg cagcgacagg tgcggctcat gatgtttgtt
     3361 tttacgatca gcgagcttgt ttttctgccc aaaacatata ttacatggga aatcattatg
     3421 aggaatttaa gttagcgttg atagaaaaac ttaatctata tgcgcatata ttaccgaatg
     3481 ccaaaaaaga ttttgatgaa aaggcggcct attctttagt tcaaaaagaa agcctgtttg
     3541 ctggattaaa agtagaggtg gatattcatc aacgttggat gattattgag tcaaatgcag
     3601 gtgtggaatt taatcaacca cttggcagat gtgtgtacct tcatcacgtc gataatattg
     3661 agcaaatatt gccttatgtt caaaaaaata agacgcaaac catatctatt tttccttggg
     3721 agtcatcatt taaatatcga gatgcgttag cattaaaagg tgcggaaagg attgtagaag
     3781 caggaatgaa taacatattt cgagttggtg gatctcatga cggaatgaga ccgttgcaac
     3841 gattagtgac atatatttct catgaaaggc catctaacta tacggctaag gatgttgcgg
     3901 ttgaaataga acagactcga ttcctggaag aagataagtt ccttgtattt gtcccataat
     3961 aggtaaaaag tatggaaaat gaatcaaaat ataaaaccat cgaccacgtt atttgtgttg
     4021 aaggaaataa aaaaattcat gtttgggaaa cgctgccaga agaaaacagc ccaaagagaa
     4081 agaatgccat tattattgcg tctggttttg cccgcaggat ggatcatttt gctggtctgg
     4141 cggaatattt atcgcggaat ggatttcatg tgatccgcta tgattcgctt caccatgttg
     4201 gcttgagttc agggacaatt gatgaattta caatgtctat aggaaagcag agcttgttag
     4261 cagtggttga ttggttaact acacgaaaaa taaataactt cggtatgttg gcttccagct
     4321 tatctgcgcg gatagcttat gcaagcctat ctgaaatcaa tgcttcgttt ttaatcaccg
     4381 cagtcggtgt tgttaactta agatattctc ttgaaagagc tttagggttt gattatctca
     4441 gtctacccat taatgaattg ccggataatc tggattttga aggccataaa ttgggtgctg
     4501 aagtctttgc gagagattgt cttgattttg gttgggaaga tttagcttct acaattaata
     4561 acatgatgta tcttgatata ccgtttattg cttttactgc aaataacgat aattgggtca
     4621 agcaagatga agttatcaca ttgttatcaa atattcgtag taatcgatgc aagatatatt
     4681 ctttgttagg aagttcgcat gacttgagtg aaaatttagt ggtcctgcgc aatttttatc
     4741 aatcggttac gaaagccgct atcgcgatgg ataatgatca tctggatatt gatgttgata
     4801 ttactgaacc gtcatttgaa catttaacta ttgcgacagt caatgaacgc cgaatgagaa
     4861 ttgagattga aaatcaagca atttctctgt cttaaaatct attgagatat tctatcactc
     4921 aaatagcaat ataaggactc tctatgaaat ttggaaactt tttgcttaca taccaacctc
     4981 cccaattttc tcaaacagag gtaatgaaac gtttggttaa attaggtcgc atctctgagg
     5041 agtgtggttt tgataccgta tggttactgg agcatcattt cacggagttt ggtttgcttg
     5101 gtaaccctta tgtcgctgct gcatatttac ttggcgcgac taaaaaattg aatgtaggaa
     5161 ctgccgctat tgttcttccc acagcccatc cagtacgcca acttgaagat gtgaatttat
     5221 tggatcaaat gtcaaaagga cgatttcggt ttggtatttg ccgagggctt tacaacaagg
     5281 actttcgcgt attcggcaca gatatgaata acagtcgcgc cttagcggaa tgctggtacg
     5341 ggctgataaa gaatggcatg acagagggat atatggaagc tgataatgaa catatcaagt
     5401 tccataaggt aaaagtaaac cccgcggcgt atagcagagg tggcgcaccg gtttatgtgg
     5461 tggctgaatc agcttcgacg actgagtggg ctgctcaatt tggcctaccg atgatattaa
     5521 gttggattat aaatactaac gaaaagaaag cacaacttga gctttataat gaagtggctc
     5581 aagaatatgg gcacgatatt cataatatcg accattgctt atcatatata acatctgtag
     5641 atcatgactc aattaaagcg aaagagattt gccggaaatt tctggggcat tggtatgatt
     5701 cttatgtgaa tgctacgact atttttgatg attcagacca aacaagaggt tatgatttca
     5761 ataaagggca gtggcgtgac tttgtattaa aaggacataa agatactaat cgccgtattg
     5821 attacagtta cgaaatcaat cccgtgggaa cgccgcagga atgtattgac ataattcaaa
     5881 aagacattga tgctacagga atatcaaata tttgttgtgg atttgaagct aatggaacag
     5941 tagacgaaat tattgcttcc atgaagctct tccagtctga tgtcatgcca tttcttaaag
     6001 aaaaacaacg ttcgctatta tattagctaa ggagaaagaa atgaaatttg gattgttctt
     6061 ccttaacttc atcaattcaa caactgttca agaacaaagt atagttcgta tgcaggaaat
     6121 aacggagtat gttgataagt tgaattttga acagatttta gtgtatgaaa atcatttttc
     6181 agataatggt gttgtcggcg ctcctctgac tgtttctggt tttctgctcg gtttaacaga
     6241 gaaaattaaa attggttcat taaatcacat cattacaact catcatcctg tccgcatagc
     6301 ggaggaagcg tgcttattgg atcagttaag tgaagggaga tttattttag ggtttagtga
     6361 ttgcgaaaaa aaagatgaaa tgcatttttt taatcgcccg gttgaatatc aacagcaact
     6421 atttgaagag tgttatgaaa tcattaacga tgctttaaca acaggctatt gtaatccaga
     6481 taacgatttt tatagcttcc ctaaaatatc tgtaaatccc catgcttata cgccaggcgg
     6541 acctcggaaa tatgtaacag caaccagtca tcatattgtt gagtgggcgg ccaaaaaagg
     6601 tattcctctc atctttaagt gggatgattc taatgatgtt agatatgaat atgctgaaag
     6661 atataaagcc gttgcggata aatatgacgt tgacctatca gagatagacc atcagttaat
     6721 gatattagtt aactataacg aagatagtaa taaagctaaa caagagacgc gtgcatttat
     6781 tagtgattat gttcttgaaa tgcaccctaa tgaaaatttc gaaaataaac ttgaagaaat
     6841 aattgcagaa aacgctgtcg gaaattatac ggagtgtata actgcggcta agttggcaat
     6901 tgaaaagtgt ggtgcgaaaa gtgtattgct gtcctttgaa ccaatgaatg atttgatgag
     6961 ccaaaaaaat gtaatcaata ttgttgatga taatattaag aagtaccaca tggaatatac
     7021 ctaatagatt tcgagttgca gcgaggcggc aagtgaacga atccccagga gcatagataa
     7081 ctatgtgact ggggtgagtg aaagcagcca acaaagcagc agcttgaaag atgaagggta
     7141 taaaagagta tgacagcagt gctgccatac tttctaatat tatcttgagg agtaaaacag
     7201 gtatgacttc atatgttgat aaacaagaaa ttacagcaag ctcagaaatt gatgatttga
     7261 ttttttcgag cgatccatta gtgtggtctt acgacgagca ggaaaaaatc agaaagaaac
     7321 ttgtgcttga tgcatttcgt aatcattata aacattgtcg agaatatcgt cactactgtc
     7381 aggcacacaa agtagatgac aatattacgg aaattgatga catacctgta ttcccaacat
     7441 cggtttttaa gtttactcgc ttattaactt ctcaggaaaa cgagattgaa agttggttta
     7501 ccagtagcgg cacgaatggt ttaaaaagtc aggtggcgcg tgacagatta agtattgaga
     7561 gactcttagg ctctgtgagt tatggcatga aatatgttgg tagttggttt gatcatcaaa
     7621 tagaattagt caatttggga ccagatagat ttaatgctca taatatttgg tttaaatatg
     7681 ttatgagttt ggtggaattg ttatatccta cgacatttac cgtaacagaa gaacgaatag
     7741 attttgttaa aacattgaat agtcttgaac gaataaaaaa tcaagggaaa gatctttgtc
     7801 ttattggttc gccatacttt atttatttac tctgccatta tatgaaagat aaaaaaatct
     7861 cattttctgg agataaaagc ctttatatca taaccggagg cggctggaaa agttacgaaa
     7921 aagaatctct gaaacgtgat gatttcaatc atcttttatt tgatactttc aatctcagtg
     7981 atattagtca gatccgagat atatttaatc aagttgaact caacacttgt ttctttgagg
     8041 atgaaatgca gcgtaaacat gttccgccgt gggtatatgc gcgagcgctt gatcctgaaa
     8101 cgttgaaacc tgtacctgat ggaacgccgg ggttgatgag ttatatggat gcgtcagcaa
     8161 ccagttatcc agcatttatt gttaccgatg atgtcgggat aattagcaga gaatatggta
     8221 agtatcccgg cgtgctcgtt gaaattttac gtcgcgtcaa tacgaggacg cagaaagggt
     8281 gtgctttgag cttaaccgaa gcgtttgata gttgatatcc tttgcctaat tgtaagtgga
     8341 atgcttgcgt tatataaatc tgaatgacat ctacacttta caaaattctc caaaacatcc
     8401 acatttgggt acttgataga ggtttatggg gttggtttaa cattgttctc attgttatta
     8461 ttggctcaaa gcaaaaggag ataacatgaa aaaattggca gttatgcttg cattgggaat
     8521 gattagcttt ggtgcaatgg cagttgatgg gtataaagat gcaaagtttg gcatgacaga
     8581 agaagagttt ctttcgaaga ggttatgtga ttttgaaaaa tttgagggag attctcgaat
     8641 agaagaagta tcactttatt catgttctga cttttcgttt gctaacaaaa agcgtgaagc
     8701 aatggcattt tttttaaatg ggaaatttaa aagattagag attaatattg gcagacttgt
     8761 gaagccagta agcaaatcgt taacgaaaaa gtacggagat ggatcatcgt atccatcaaa
     8821 agaagaattt gagaacgcgc taaaatacaa tggaactatg tctataggtt atgataataa
     8881 tacggtatta gttgatatac atataatatg tggcaaagaa ggcatagaaa ccagtcaact
     8941 gatttatacg agtccagatg tttatacgct cccagatttc ggagaaaaaa tccaggaatt
     9001 aaagggatta aaggaattgg gtacctcgcg aaggccttgc aggccaacca gataagtgaa
     9061 atctagttcc aaactatttt gtcattttta attttcgtat tagcttacga cgctacaccc
     9121 agttcccatc tattttgtca ctcttcccta aataatcctt aaaaactcca tttccacccc
     9181 tcccagttcc caactatttt gtccgcccac agcggggcat ttttcttcct gttatgtttg
     9241 ggcgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     9301 ttccgcttcc tcgctcactg acccgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     9361 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     9421 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     9481 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     9541 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     9601 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     9661 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     9721 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     9781 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     9841 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     9901 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     9961 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    10021 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    10081 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    10141 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    10201 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    10261 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    10321 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    10381 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    10441 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    10501 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    10561 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    10621 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    10681 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    10741 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    10801 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    10861 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    10921 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    10981 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    11041 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    11101 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    11161 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    11221 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
    11281 tatcacgagg ccctttcgtc
//
