LOCUS       40924_44983        7656 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUC18-mini-Tn7T-Gm-lacZ, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7656)
  AUTHORS   Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer 
            RR, Schweizer HP.
  TITLE     A Tn7-based broad-range bacterial cloning and expression system
  JOURNAL   Nat. Methods 2 (6), 443-448 (2005)
  PUBMED    15908923
REFERENCE   2  (bases 1 to 7656)
  AUTHORS   Choi KH, Gaynor J, Karkhoff-Schweizer RR, Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-APR-2004) Microbiology, Immunology and Pathology, 
            Colorado State University, Fort Collins, CO 80523, USA
REFERENCE   3  (bases 1 to 7656)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7656)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Methods"; date: "2005"; volume: "2"; issue: "6"; pages: "443-448"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-APR-2004) Microbiology, Immunology and Pathology, Colorado State
            University, Fort Collins, CO 80523, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  382..580
                     /mobile_element_type="transposon:Tn7R"
                     /label=n7R
                     /note="right end of Tn7"
     primer_bind     585..601
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      629..723
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      826..912
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    958..1005
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(1138..1668)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(1857..1885)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     misc_feature    1926..1973
                     /note="FRT; Flp recombinase target site"
     protein_bind    1926..1973
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     misc_feature    2000..2079
                     /note="MCS; multiple cloning site"
     misc_feature    2103..2179
                     /label=RNase III processing site
                     /note="RNase III processing site"
     CDS             2243..5314
                     /label=lacZ
                     /note="beta-galactosidase"
     mobile_element  complement(5402..5567)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      complement(5837..6425)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6599..7456)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7457..7561)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct agaggaccag ccgcgtaacc tggcaaaatc ggttacggtt gagtaataaa
      361 tggatgccct gcgtaagcgg gtgtgggcgg acaataaagt cttaaactga acaaaataga
      421 tctaaactat gacaataaag tcttaaacta gacagaatag ttgtaaactg aaatcagtcc
      481 agttatgctg tgaaaaagca tactggactt ttgttatggc taaagcaaac tcttcatttt
      541 ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg ggttcgaggt cgacggtatc
      601 gataagctag cttaattagc tgagcttgga ctcctgttga tagatccagt aatgacctca
      661 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag
      721 aatccaagct agactgcgat gagtggcagg gcggggcgta atttttttaa ggcagttatt
      781 ggtgccctta aacgcctggg gtaatgactc tctagcttga ggcatcaaat aaaacgaaag
      841 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
      901 agtaggacaa atccgccgct aggagcttgc ggcccggacg atcgaattgg ggatcttgaa
      961 gtacctattc cgaagttcct attctctaga aagtatagga acttcagagc gcttttgaag
     1021 ctgatgtgct taaaaactta ctcaatggaa ttctagatcg aattggccgc ggcgttgtga
     1081 caatttaccg aacaactccg cggccgggaa gccgatctcg gcttgaacga attgttaggt
     1141 ggcggtactt gggtcgatat caaagtgcat cacttcttcc cgtatgccca actttgtata
     1201 gagagccact gcgggatcgt caccgtaatc tgcttgcacg tagatcacat aagcaccaag
     1261 cgcgttggcc tcatgcttga ggagattgat gagcgcggtg gcaatgccct gcctccggtg
     1321 ctcgccggag actgcgagat catagatata gatctcacta cgcggctgct caaacttggg
     1381 cagaacgtaa gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag caagcgcgat
     1441 gaatgtctta ctacggagca agttcccgag gtaatcggag tccggctgat gttgggagta
     1501 ggtggctacg tctccgaact cacgaccgaa aagatcaaga gcagcccgca tggatttgac
     1561 ttggtcaggg ccgagcctac atgtgcgaat gatgcccata cttgagccac ctaactttgt
     1621 tttagggcga ctgccctgct gcgtaacatc gttgctgctg cgtaacatcg ttgctgctcc
     1681 ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag
     1741 actgtacaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc
     1801 tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagtt
     1861 tacgaaccga acaggcttat gtcaattcga tccattgctg ttgacaaagg gaatcagggg
     1921 atcttgaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttcagagcgc
     1981 ttttgaagct aattcgatca tgcatgagct cactagtgga tcccccgggc tgcaggaatt
     2041 cctcgagaag cttgggcccg gtacctcgcg aagggatcct aactaactag cgatcccgac
     2101 tcactataga gggacaaact caaggtcatt cgcaagagtg gcctttatga ttgaccttct
     2161 tccggttaat acgaccggga tcgagatcct aggtaggtag gggcgcggca ttttaacttt
     2221 ctttatcaca caggaaacag ctatgaccat gattacggat tcactggccg tcgttttaca
     2281 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     2341 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     2401 cagcctgaat ggcgaatggc gctttgcctg gtttccggca ccagaagcgg tgccggaaag
     2461 ctggctggag tgcgatcttc ctgaggccga tactgtcgtc gtcccctcaa actggcagat
     2521 gcacggttac gatgcgccca tctacaccaa cgtaacctat cccattacgg tcaatccgcc
     2581 gtttgttccc acggagaatc cgacgggttg ttactcgctc acatttaatg ttgatgaaag
     2641 ctggctacag gaaggccaga cgcgaattat ttttgatggc gttaactcgg cgtttcatct
     2701 gtggtgcaac gggcgctggg tcggttacgg ccaggacagt cgtttgccgt ctgaatttga
     2761 cctgagcgca tttttacgcg ccggagaaaa ccgcctcgcg gtgatggtgc tgcgttggag
     2821 tgacggcagt tatctggaag atcaggatat gtggcggatg agcggcattt tccgtgacgt
     2881 ctcgttgctg cataaaccga ctacacaaat cagcgatttc catgttgcca ctcgctttaa
     2941 tgatgatttc agccgcgctg tactggaggc tgaagttcag atgtgcggcg agttgcgtga
     3001 ctacctacgg gtaacagttt ctttatggca gggtgaaacg caggtcgcca gcggcaccgc
     3061 gcctttcggc ggtgaaatta tcgatgagcg tggtggttat gccgatcgcg tcacactacg
     3121 tctgaacgtc gaaaacccga aactgtggag cgccgaaatc ccgaatctct atcgtgcggt
     3181 ggttgaactg cacaccgccg acggcacgct gattgaagca gaagcctgcg atgtcggttt
     3241 ccgcgaggtg cggattgaaa atggtctgct gctgctgaac ggcaagccgt tgctgattcg
     3301 aggcgttaac cgtcacgagc atcatcctct gcatggtcag gtcatggatg agcagacgat
     3361 ggtgcaggat atcctgctga tgaagcagaa caactttaac gccgtgcgct gttcgcatta
     3421 tccgaaccat ccgctgtggt acacgctgtg cgaccgctac ggcctgtatg tggtggatga
     3481 agccaatatt gaaacccacg gcatggtgcc aatgaatcgt ctgaccgatg atccgcgctg
     3541 gctaccggcg atgagcgaac gcgtaacgcg aatggtgcag cgcgatcgta atcacccgag
     3601 tgtgatcatc tggtcgctgg ggaatgaatc aggccacggc gctaatcacg acgcgctgta
     3661 tcgctggatc aaatctgtcg atccttcccg cccggtgcag tatgaaggcg gcggagccga
     3721 caccacggcc accgatatta tttgcccgat gtacgcgcgc gtggatgaag accagccctt
     3781 cccggctgtg ccgaaatggt ccatcaaaaa atggctttcg ctacctggag agacgcgccc
     3841 gctgatcctt tgcgaatacg cccacgcgat gggtaacagt cttggcggtt tcgctaaata
     3901 ctggcaggcg tttcgtcagt atccccgttt acagggcggc ttcgtctggg actgggtgga
     3961 tcagtcgctg attaaatatg atgaaaacgg caacccgtgg tcggcttacg gcggtgattt
     4021 tggcgatacg ccgaacgatc gccagttctg tatgaacggt ctggtctttg ccgaccgcac
     4081 gccgcatcca gcgctgacgg aagcaaaaca ccagcagcag tttttccagt tccgtttatc
     4141 cgggcaaacc atcgaagtga ccagcgaata cctgttccgt catagcgata acgagctcct
     4201 gcactggatg gtggcgctgg atggtaagcc gctggcaagc ggtgaagtgc ctctggatgt
     4261 cgctccacaa ggtaaacagt tgattgaact gcctgaacta ccgcagccgg agagcgccgg
     4321 gcaactctgg ctcacagtac gcgtagtgca accgaacgcg accgcatggt cagaagccgg
     4381 gcacatcagc gcctggcagc agtggcgtct ggcggaaaac ctcagtgtga cgctccccgc
     4441 cgcgtcccac gccatcccgc atctgaccac cagcgaaatg gatttttgca tcgagctggg
     4501 taataagcgt tggcaattta accgccagtc aggctttctt tcacagatgt ggattggcga
     4561 taaaaaacaa ctgctgacgc cgctgcgcga tcagttcacc cgtgcaccgc tggataacga
     4621 cattggcgta agtgaagcga cccgcattga ccctaacgcc tgggtcgaac gctggaaggc
     4681 ggcgggccat taccaggccg aagcagcgtt gttgcagtgc acggcagata cacttgctga
     4741 tgcggtgctg attacgaccg ctcacgcgtg gcagcatcag gggaaaacct tatttatcag
     4801 ccggaaaacc taccggattg atggtagtgg tcaaatggcg attaccgttg atgttgaagt
     4861 ggcgagcgat acaccgcatc cggcgcggat tggcctgaac tgccagctgg cgcaggtagc
     4921 agagcgggta aactggctcg gattagggcc gcaagaaaac tatcccgacc gccttactgc
     4981 cgcctgtttt gaccgctggg atctgccatt gtcagacatg tataccccgt acgtcttccc
     5041 gagcgaaaac ggtctgcgct gcgggacgcg cgaattgaat tatggcccac accagtggcg
     5101 cggcgacttc cagttcaaca tcagccgcta cagtcaacag caactgatgg aaaccagcca
     5161 tcgccatctg ctgcacgcgg aagaaggcac atggctgaat atcgacggtt tccatatggg
     5221 gattggtggc gacgactcct ggagcccgtc agtatcggcg gagttccagc tgagcgccgg
     5281 tcgctaccat taccagttgg tctggtgtca aaaataataa taaccgggca ggccatgtct
     5341 gcccgtattt cgcgtaagga aatccattat gtactattta aaatcaagct ccttgcaggc
     5401 caaccagata agtgaaatct agttccaaac tattttgtca tttttaattt tcgtattagc
     5461 ttacgacgct acacccagtt cccatctatt ttgtcactct tccctaaata atccttaaaa
     5521 actccatttc cacccctccc agttcccaac tattttgtcc gcccacagcg gggcattttt
     5581 cttcctgtta tgtttgggcg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     5641 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     5701 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     5761 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     5821 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     5881 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     5941 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     6001 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     6061 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     6121 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     6181 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     6241 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     6301 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     6361 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     6421 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     6481 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     6541 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     6601 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     6661 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     6721 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     6781 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     6841 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     6901 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     6961 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     7021 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     7081 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     7141 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     7201 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     7261 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     7321 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     7381 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     7441 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     7501 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     7561 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     7621 cctataaaaa taggcgtatc acgaggccct ttcgtc
//
