LOCUS       40924_44968        3988 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUC18-mini-Tn7-Gm, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer 
            RR, Schweizer HP.
  TITLE     A Tn7-based broad-range bacterial cloning and expression system
  JOURNAL   Nat. Methods 2 (6), 443-448 (2005)
  PUBMED    15908923
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Choi K-H., Gaynor JB, Karkhoff-Schweizer RR, Schweizer HP.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2004) Microbiology, Immunology and Pathology, 
            Colorado State University, Fort Collins, CO 80523, USA
REFERENCE   3  (bases 1 to 3988)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3988)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Methods"; date: "2005"; volume: "2"; issue: "6"; pages: "443-448"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (06-MAY-2004) Microbiology, Immunology and Pathology, Colorado State
            University, Fort Collins, CO 80523, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3988
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   382..580
                     /note="Tn7R; right end of Tn7"
     protein_bind    600..647
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(780..1310)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(1499..1527)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     misc_feature    1568..1615
                     /label=FRT, Flp recombinase target site
                     /note="FRT, Flp recombinase target site"
     protein_bind    1568..1615
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     mobile_element  complement(1734..1899)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      complement(2169..2757)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2931..3788)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3789..3893)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct agaggaccag ccgcgtaacc tggcaaaatc ggttacggtt gagtaataaa
      361 tggatgccct gcgtaagcgg gtgtgggcgg acaataaagt cttaaactga acaaaataga
      421 tctaaactat gacaataaag tcttaaacta gacagaatag ttgtaaactg aaatcagtcc
      481 agttatgctg tgaaaaagca tactggactt ttgttatggc taaagcaaac tcttcatttt
      541 ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg ggtccgaatt ggggatcttg
      601 aagtacctat tccgaagttc ctattctcta gaaagtatag gaacttcaga gcgcttttga
      661 agctgatgtg cttaaaaact tactcaatgg aattctagat cgaattggcc gcggcgttgt
      721 gacaatttac cgaacaactc cgcggccggg aagccgatct cggcttgaac gaattgttag
      781 gtggcggtac ttgggtcgat atcaaagtgc atcacttctt cccgtatgcc caactttgta
      841 tagagagcca ctgcgggatc gtcaccgtaa tctgcttgca cgtagatcac ataagcacca
      901 agcgcgttgg cctcatgctt gaggagattg atgagcgcgg tggcaatgcc ctgcctccgg
      961 tgctcgccgg agactgcgag atcatagata tagatctcac tacgcggctg ctcaaacttg
     1021 ggcagaacgt aagccgcgag agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg
     1081 atgaatgtct tactacggag caagttcccg aggtaatcgg agtccggctg atgttgggag
     1141 taggtggcta cgtctccgaa ctcacgaccg aaaagatcaa gagcagcccg catggatttg
     1201 acttggtcag ggccgagcct acatgtgcga atgatgccca tacttgagcc acctaacttt
     1261 gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tgcgtaacat cgttgctgct
     1321 ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat
     1381 agactgtaca aaaaaacagt cataacaagc catgaaaacc gccactgcgc cgttaccacc
     1441 gctgcgttcg gtcaaggttc tggaccagtt gcgtgagcgc atacgctact tgcattacag
     1501 tttacgaacc gaacaggctt atgtcaattc gatccattgc tgttgacaaa gggaatcagg
     1561 ggatcttgaa gttcctattc cgaagttcct attctctaga aagtatagga acttcagagc
     1621 gcttttgaag ctaattcgga cgatatcatg catgagctca ctagtggatc ccccgggctg
     1681 caggaattcc tcgagaagct tgggcccggt acctcgcgaa ggccttgcag gccaaccaga
     1741 taagtgaaat ctagttccaa actattttgt catttttaat tttcgtatta gcttacgacg
     1801 ctacacccag ttcccatcta ttttgtcact cttccctaaa taatccttaa aaactccatt
     1861 tccacccctc ccagttccca actattttgt ccgcccacag cggggcattt ttcttcctgt
     1921 tatgtttggg cgctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     1981 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     2041 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     2101 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     2161 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     2221 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     2281 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     2341 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     2401 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     2461 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     2521 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     2581 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     2641 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     2701 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     2761 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     2821 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     2881 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     2941 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     3001 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     3061 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     3121 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     3181 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     3241 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     3301 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     3361 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     3421 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     3481 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     3541 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     3601 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     3661 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     3721 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     3781 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     3841 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     3901 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     3961 aataggcgta tcacgaggcc ctttcgtc
//
