LOCUS       V013864                 4605 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013864
VERSION     V013864
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             475..1191
                     /gene="lasR"
                     /label="Transcriptional activator protein LasR"
                     /note="Transcriptional activator protein LasR from
                     Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP
                     104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
                     Accession#: P25084"
     terminator      1206..1277
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1293..1320
                     /label="T7Te terminator"
                     /note="phage T7 early transcription terminator"
     CDS             1505..2221
                     /label="EGFP"
                     /note="enhanced GFP"
     terminator      complement(2242..2285)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     complement(2384..2400)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2408..2424)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2432..2462)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2477..2498)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2786..3374)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3548..4405)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4406..4510)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctttttaca gctagctcag
      421 tcctaggtat tatgctaggt agcatgcaaa agaggagaaa cctctagaaa taatatggcc
      481 ttggttgacg gttttcttga gctggaacgc tcaagtggaa aattggagtg gagcgccatc
      541 ctccagaaga tggcgagcga ccttggattc tcgaagatcc tgttcggcct gttgcctaag
      601 gacagccagg actacgagaa cgccttcatc gtcggcaact acccggccgc ctggcgcgag
      661 cattacgacc gggctggcta cgcgcgggtc gacccgacgg tcagtcactg tacccagagc
      721 gtactgccga ttttctggga accgtccatc taccagacgc gaaagcagca cgagttcttc
      781 gaggaagcct cggccgccgg cctggtgtat gggctgacca tgccgctgca tggtgctcgc
      841 ggcgaactcg gcgcgctgag cctcagcgtg gaagcggaaa accgggccga ggccaaccgt
      901 ttcatagagt cggtcctgcc gaccctgtgg atgctcaagg actacgcact gcaaagcggt
      961 gccggactgg ccttcgaaca tccggtcagc aaaccggtgg ttctgaccag ccgggagaag
     1021 gaagtgttgc agtggtgcgc catcggcaag accagttggg agatatcggt tatctgcaac
     1081 tgctcggaag ccaatgtgaa cttccatatg ggaaatattc ggcggaagtt cggtgtgacc
     1141 tcccgccgcg tagcggccat tatggccgtt aatttgggtc ttattactct ctaataacca
     1201 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     1261 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     1321 tttataagtc agtagcatgc atgacccctt tccgaaacga aacaagttgg attttgcacc
     1381 taccagaact ggtagttctg acctgtggct atcttcgaag gcatcgatat tatgcacatt
     1441 ggaactcttc atgacataac gccgagagtc gatatggaaa gaggagaaag ccatatctga
     1501 attcatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     1561 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     1621 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     1681 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     1741 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     1801 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     1861 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     1921 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     1981 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     2041 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     2101 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     2161 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     2221 gtaaggtacc ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     2281 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     2341 gggcctttct gcgtttatag gatccccgga gctcgtaatc atggtcatag ctgtttcctg
     2401 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     2461 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     2521 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     2581 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     2641 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     2701 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     2761 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     2821 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     2881 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     2941 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca aagctcacgc tgtaggtatc
     3001 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3061 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3121 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3181 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     3241 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3301 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3361 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     3421 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     3481 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     3541 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     3601 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     3661 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     3721 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     3781 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     3841 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     3901 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     3961 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     4021 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     4081 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     4141 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     4201 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     4261 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     4321 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     4381 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     4441 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     4501 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     4561 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc
//
