LOCUS       V015048                 4034 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V015048
VERSION     V015048
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4034
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    complement(399..455)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(465..481)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(489..505)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(513..543)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(558..579)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             1249..1983
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     rep_origin      complement(2215..2803)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2977..3834)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3835..3939)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
      421 actctagagg atccccgggt accgagctcg aattcgtaat catggtcata gctgtttcct
      481 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      541 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      601 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
      661 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
      721 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
      781 gaatcagggg ataacgcagg aaagaacatg ttctctcttt atactgcaaa agaagcgcag
      841 ttattcacga ttaggataac tgtttgagag agcctaaggg cttgaccctt gatggtttaa
      901 gcaccgctat gcgtgcggga tccccttaga agcaaactta gagtgtgttg atagtgcatt
      961 atcttaaaat tttgtataat aggaattgaa gttaaattag atgctaaaaa taggaattga
     1021 agttaaatta gatgctaaaa atttgtaatt aagaaggagg gattcgtcat gttggtattc
     1081 caaatgcgta atgtagataa aacatctact gttttgaaac agactaaaaa cagtgattac
     1141 gcagataaat aaatacgtta gattaattcc taccagtgac taatcttatg actttttaaa
     1201 cagataacta aaattacaaa caaatcgttt aacttcagga gagattacat gaacaaaaat
     1261 ataaaatatt ctcaaaactt tttaacgagt gaaaaagtac tcaaccaaat aataaaacaa
     1321 ttgaatttaa aagaaaccga taccgtttac gaaattggaa caggtaaagg gcatttaacg
     1381 acgaaactgg ctaaaataag taaacaggta acgtctattg aattagacag tcatctattc
     1441 aacttatcgt cagaaaaatt aaaactgaat actcgtgtca ctttaattca ccaagatatt
     1501 ctacagtttc aattccctaa caaacagagg tataaaattg ttgggaatat tccttacaat
     1561 ttaagcacac aaattattaa aaaagtggtt tttgaaagcc gtgcgtctga catctatctg
     1621 actgttgaag aaggattcta caagcgtacc ttggatattc accgaacact agggttgctc
     1681 ttgcacactc aagtctcgat tcagcaattg cttaagctgc cagcggaatg ctttcatcct
     1741 aaaccaaaag taaacagtgt cttaataaaa cttacccgcc ataccacaga tgttccagat
     1801 aaatattgga agctatataa gtactttgtt tcaaaatggg tcaatcgaga atatcgtcaa
     1861 ctgtttacta aaaatcagtt tcgtcaagca atgaaacacg ccaaagtaaa caatttaagt
     1921 accattactt atgagcaagt attgtctatt tttaatagtt atctattatt taacgggagg
     1981 aaataattct atgagtcgct tttttaaatt tggaaagtta cacgttacta aagggaatgg
     2041 agaccggggt cgacttcaat agagttctta acgttaatcc gaaaaaaact aacgttaata
     2101 ttaaaaaata agatccgctt gtgaattatg tataatttga ttagactaaa gaataggagg
     2161 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     2221 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     2281 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     2341 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     2401 gctttctcaa agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     2461 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     2521 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     2581 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     2641 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     2701 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     2761 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     2821 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     2881 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     2941 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     3001 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     3061 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     3121 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     3181 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     3241 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     3301 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     3361 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     3421 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     3481 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     3541 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     3601 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     3661 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     3721 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     3781 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     3841 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     3901 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     3961 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     4021 gaggcccttt cgtc
//
