LOCUS 40924_44918 4199 bp DNA circular SYN 18-DEC-2018 DEFINITION BiFC expression vector pUC-triRV, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4199) AUTHORS Offenborn JN, Waadt R, Kudla J. TITLE Visualization and translocation of ternary Calcineurin-A/Calcineurin-B/Calmodulin-2 protein complexes by dual-color trimolecular fluorescence complementation JOURNAL New Phytol. (2015) In press PUBMED 25919910 REFERENCE 2 (bases 1 to 4199) AUTHORS Offenborn JN, Waadt R, Kudla J. TITLE Direct Submission JOURNAL Submitted (22-MAY-2015) University of Muenster, Institute for Biology and Plant Biotechnology, Schlossplatz 7, Muenster 48149, Germany REFERENCE 3 (bases 1 to 4199) TITLE Direct Submission REFERENCE 4 (bases 1 to 4199) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "New Phytol. (2015) In press" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (22-MAY-2015) University of Muenster, Institute for Biology and Plant Biotechnology, Schlossplatz 7, Muenster 48149, Germany" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..4199 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 96..200 /label=AmpR promoter CDS 201..1058 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 1232..1820 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 2108..2129 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 2144..2174 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2182..2198 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2206..2222 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" promoter 2751..3096 /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" misc_feature 3114..3344 /label=mCherry 160-236 /note="mCherry 160-236" CDS 3345..3371 /codon_start=1 /label=HA /note="HA (human influenza hemagglutinin) epitope tag" /translation="YPYDVPDYA" misc_feature 3372..3422 /label=MCS2 /note="MCS2" CDS 3426..3452 /codon_start=1 /label=HA /note="HA (human influenza hemagglutinin) epitope tag" /translation="YPYDVPDYA" CDS 3453..3704 /codon_start=1 /label=VC155 /note="C-terminal fragment of mVenus for use in bimolecular fluorescence complementation (BiFC) (Kodama and Hu, 2010)" /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK" terminator 3724..3976 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" primer_bind complement(3985..4001) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" ORIGIN 1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 1621 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc 2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg 2221 accatgatta cgccaagctt agcttgcatg cctgcaggtc cccagattag ccttttcaat 2281 ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg tctcatcaag 2341 acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa ggttaaagat 2401 gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat atttctcaag 2461 atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc aaggcaagta 2521 atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat ggagtcaaag 2581 attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt catacagagt 2641 ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca cgacacactt 2701 gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat tgagactttt 2761 caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat ctgtcacttt 2821 attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga 2881 aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg 2941 aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt 3001 gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc 3061 tctatataag gaagttcatt tcatttggag agaacacggg ggactctaga atgggcgccc 3121 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg 3181 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca 3241 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg 3301 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaccca tacgatgttc 3361 cagattacgc tgggcccagg cctactagtg gatccgtcga cctcgagggt accgctcccg 3421 ggatgtaccc atacgatgtt ccagattacg ctgacaagca gaagaacggc atcaaggcca 3481 acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc 3541 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc 3601 agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg 3661 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaagag ctcgaatttc 3721 cccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt 3781 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa 3841 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa 3901 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca 3961 tctatgttac tagatcggga attcactggc cgtcgtttta caacgtcgtg actgggaaaa 4021 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa 4081 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg 4141 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggt //