LOCUS       62056_21900        2814 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..2814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(597..613)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(621..637)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(645..675)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(690..711)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(999..1587)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1761..2618)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2619..2723)
                     /label=AmpR promoter
ORIGIN
        1 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gactgtcaca
       61 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
      121 ggcgggtgtc ggggctggct taattatgcg gcatcagagc agatggtaga cagagtgcac
      181 cagatgcggt gagaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat
      241 tcgccattca ggctgcgcag ctgttgggaa gggcggtcgg tgcgggcctc ttcgctatta
      301 cgccagctgg cgaaaggcgg atgtgctgcc aggcgattca gttgggtaac gccagggttt
      361 tcccagtcac gacgttgcag aacgacggcc agagagttag aggacggaac ctcgcgaata
      421 caacgagata tcgggtcccg acacgtgaaa tggtcatgtg tggcggttca ctatatgtta
      481 aaccaggtgg aacctcatca ggagatgcca caactgctta tgctaatagt gtttttaaca
      541 tttggacacg ggcacgacga gaacacaggc ctgcgtgcag agatggcgta atcatggtca
      601 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
      661 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
      721 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
      781 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
      841 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
      901 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
      961 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1021 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1081 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1141 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1201 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1261 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1321 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1381 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     1441 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1501 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     1561 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     1621 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     1681 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     1741 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     1801 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     1861 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     1921 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     1981 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     2041 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     2101 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     2161 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     2221 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     2281 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     2341 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     2401 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     2461 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     2521 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     2581 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     2641 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     2701 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     2761 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc cttt
//
