LOCUS       62056_21895        3155 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             114..920
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      1101..1689
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    1977..1998
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             2163..2828
                     /codon_start=1
                     /label=CopGFP
                     /note="green fluorescent protein 2 from Pontellina plumata,
                     also known as ppluGFP2 (Shagin et al., 2004)"
                     /translation="MPAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGAL
                     TFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYR
                     YEAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDG
                     GYYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA
                     "
ORIGIN
        1 tgcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
       61 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc
      121 atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg
      181 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg
      241 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg
      301 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca
      361 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa
      421 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg
      481 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc
      541 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg
      601 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac
      661 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta
      721 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc
      781 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga
      841 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt
      901 tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacat tattcagatt
      961 gggcttgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     1021 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1081 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1141 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     1201 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     1261 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     1321 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     1381 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     1441 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     1501 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     1561 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     1621 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     1681 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     1741 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     1801 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     1861 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     1921 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     1981 gtgagttagc tcactcatta ggcaccccag gctgtggatt aagttttaag aggcaataaa
     2041 gattataata agtgctgcta caccatactg atgtatggca aaaccataat aatgaactta
     2101 aggaagaccc tctactagag aaagaggaga aatactagat ggagagcgac gagagcggcc
     2161 tgatgcccgc catggagatc gagtgccgca tcaccggcac cctgaacggc gtggagttcg
     2221 agctggtggg cggcggagag ggcaccccca agcagggccg catgaccaac aagatgaaga
     2281 gcaccaaagg cgccctgacc ttcagcccct acctgctgag ccacgtgatg ggctacggct
     2341 tctaccactt cggcacctac cccagcggct acgagaaccc cttcctgcac gccatcaaca
     2401 acggcggcta caccaacacc cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga
     2461 gcttcagcta ccgctacgag gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg
     2521 gcttccccga ggacagcgtg atcttcaccg acaagatcat ccgcagcaac gccaccgtgg
     2581 agcacctgca ccccatgggc gataacgtgc tggtgggcag cttcgcccgc accttcagcc
     2641 tgcgcgacgg cggctactac agcttcgtgg tggacagcca catgcacttc aagagcgcca
     2701 tccaccccag catcctgcag aacgggggcc ccatgttcgc cttccgccgc gtggaggagc
     2761 tgcacagcaa caccgagctg ggcatcgtgg agtaccagca cgccttcaag acccccatcg
     2821 ccttcgccta aagatcccgc gctcagtcgt ccaattctgc cgtggacggc accgccggac
     2881 ccggctccac cggatctcgc ccatgggcta gcggtttgaa gggtattggt cggtcagttt
     2941 cacctgattt acgtaaaaac ccgcttcggc gggtttttgc ttttggaggg gcagaaagat
     3001 gaatgactgt ctaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     3061 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     3121 tcagaggttt tcaccgtcat caccgaaacg cgcga
//
