LOCUS       62056_21885        4410 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    225..246
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        261..291
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    299..315
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     323..339
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        863..1208
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1334..1852
                     /codon_start=1
                     /label=VN173
                     /note="N-terminal fragment of mVenus for use in bimolecular
                     fluorescence complementation (BiFC) (Kodama and Hu, 2010)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     terminator      1872..2124
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2133..2149)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2623..2727
                     /label=AmpR promoter
     CDS             2728..3585
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3759..4347
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
       61 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
      121 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
      181 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
      241 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
      301 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcttgca
      361 tgcctgcagg tccccagatt agccttttca atttcagaaa gaatgctaac ccacagatgg
      421 ttagagaggc ttacgcagca ggtctcatca agacgatcta cccgagcaat aatctccagg
      481 aaatcaaata ccttcccaag aaggttaaag atgcagtcaa aagattcagg actaactgca
      541 tcaagaacac agagaaagat atatttctca agatcagaag tactattcca gtatggacga
      601 ttcaaggctt gcttcacaaa ccaaggcaag taatagagat tggagtctct aaaaaggtag
      661 ttcccactga atcaaaggcc atggagtcaa agattcaaat agaggaccta acagaactcg
      721 ccgtaaagac tggcgaacag ttcatacaga gtctcttacg actcaatgac aagaagaaaa
      781 tcttcgtcaa catggtggag cacgacacac ttgtctactc caaaaatatc aaagatacag
      841 tctcagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcc ggaaacctcc
      901 tcggattcca ttgcccagct atctgtcact ttattgtgaa gatagtggaa aaggaaggtg
      961 gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat gcctctgccg
     1021 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc
     1081 caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg
     1141 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg
     1201 agagaacacg ggggactcta gagttaaccg ggctcaggcc tggcgcgcca ctagtggatc
     1261 catcgatagt actgtcgacc tcgagggtac cgctcccggg atggagcaaa agttgatttc
     1321 tgaggaggat cttatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     1381 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     1441 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     1501 gccctggccc accctcgtga ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc
     1561 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     1621 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     1681 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     1741 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     1801 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg agtaggagct
     1861 cgaatttccc cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
     1921 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     1981 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     2041 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
     2101 cggtgtcatc tatgttacta gatcgggaat tcactggccg tcgttttaca acgtcgtgac
     2161 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     2221 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     2281 ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     2341 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     2401 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     2461 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     2521 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     2581 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     2641 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     2701 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     2761 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     2821 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     2881 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     2941 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     3001 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     3061 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     3121 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     3181 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     3241 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     3301 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     3361 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     3421 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     3481 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     3541 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     3601 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     3661 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     3721 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     3781 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     3841 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     3901 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     3961 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     4021 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     4081 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     4141 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     4201 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     4261 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     4321 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     4381 gccttttgct ggccttttgc tcacatgttc
//
