LOCUS       62056_21870        4140 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    225..246
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        261..291
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    299..315
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     323..339
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        863..1208
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1304..1330
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     CDS             1331..1582
                     /codon_start=1
                     /label=VC155
                     /note="C-terminal fragment of mVenus for use in bimolecular
                     fluorescence complementation (BiFC) (Kodama and Hu, 2010)"
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     terminator      1602..1854
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1863..1879)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2353..2457
                     /label=AmpR promoter
     CDS             2458..3315
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3489..4077
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
       61 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
      121 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
      181 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
      241 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
      301 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcttgca
      361 tgcctgcagg tccccagatt agccttttca atttcagaaa gaatgctaac ccacagatgg
      421 ttagagaggc ttacgcagca ggtctcatca agacgatcta cccgagcaat aatctccagg
      481 aaatcaaata ccttcccaag aaggttaaag atgcagtcaa aagattcagg actaactgca
      541 tcaagaacac agagaaagat atatttctca agatcagaag tactattcca gtatggacga
      601 ttcaaggctt gcttcacaaa ccaaggcaag taatagagat tggagtctct aaaaaggtag
      661 ttcccactga atcaaaggcc atggagtcaa agattcaaat agaggaccta acagaactcg
      721 ccgtaaagac tggcgaacag ttcatacaga gtctcttacg actcaatgac aagaagaaaa
      781 tcttcgtcaa catggtggag cacgacacac ttgtctactc caaaaatatc aaagatacag
      841 tctcagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcc ggaaacctcc
      901 tcggattcca ttgcccagct atctgtcact ttattgtgaa gatagtggaa aaggaaggtg
      961 gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat gcctctgccg
     1021 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc
     1081 caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg
     1141 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg
     1201 agagaacacg ggggactcta gagttaaccg ggctcaggcc tggcgcgcca ctagtggatc
     1261 catcgatagt actgtcgacc tcgagggtac cgctcccggg atgtacccat acgatgttcc
     1321 agattacgct gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     1381 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     1441 ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc
     1501 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     1561 cggcatggac gagctgtaca agtaagagct cgaatttccc cgatcgttca aacatttggc
     1621 aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc
     1681 tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat
     1741 gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat
     1801 agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat
     1861 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     1921 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     1981 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc
     2041 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
     2101 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     2161 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     2221 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     2281 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     2341 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     2401 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     2461 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     2521 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     2581 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     2641 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     2701 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     2761 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     2821 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     2881 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     2941 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     3001 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     3061 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     3121 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     3181 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     3241 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     3301 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     3361 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     3421 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3481 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3541 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3601 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     3661 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3721 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3781 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3841 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     3901 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     3961 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4021 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4081 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
//
