LOCUS       62056_21855        4944 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1446..1502
                     /codon_start=1
                     /label=GCN4_v4
                     /note="GCN4 peptide optimized to improve solubility while 
                     preserving binding to an scFv-GFP fusion protein (Tanenbaum
                     et al., 2014)"
                     /translation="EELLSKNYHLENEVARLKK"
     primer_bind     complement(2768..2784)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2792..2808)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2816..2846)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2861..2882)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3170..3758)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3932..4744)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRFAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        complement(4745..4849)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagactgtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gacgcgtatt gggatcctga
      421 agatcaaggc tgctgcagtg agctgtgatc gcactactgc actccagcct gggccacaga
      481 gtgaaccctg tctctaaaaa aaaataaaaa caaaacaaga gtccctgggc cctttgaata
      541 aaaagagagt aaagaggctg ggcacagtgg ctcacgcccg taatcccagc actttgggag
      601 gctgaggcag gcagatcact tgtgcccagg agtttgaggc cagcgtgggc aacatagcaa
      661 aatcccatct ctccaaaaaa aaaaaaaaaa ggaaaagaaa agaaagtgag taaaggcttt
      721 tgggttataa agagaaaggg agaaggatga tccagactag aaagaagatt attcctttct
      781 tagctcaacc tttttgctcc aaaaaaaaga ggaggaaggg gccaaacata gattatcctg
      841 caccagaggg ccagctcctt aaatctgagt ctcatcccaa ccaccatcat ttgtaaaaac
      901 caagggtgca ttcattacct gcagtgcgtg gatccctgtg gtgccatcat tatcatttga
      961 agctccatct ctgaagcctc tagctttgta aggacctaca gaccacttcc caagtcattg
     1021 caggtttatt tttgtcgcat ttgagcataa cctcagattt ggcatacaat acgaatgtca
     1081 ccttgaacag cttctccttg aatgggggcc tactctcccc aggtgcagtg agcaccgtcg
     1141 tctttacatg agtgggccta caccaccctg ctgagtaaat ccatctgttc tgtttcagta
     1201 actgtggcct ctacaaatgg gaacgggacg gaatgctaaa ggtatgccat ttgcgtacta
     1261 atttttgact ccttttagtg acccatgcta ataatgtgga accatctcct attaaaatat
     1321 tttcattttt ctaggaaaag actggtccaa aaataggagg agaaactctg ttaccaagag
     1381 gagaagaaca tgctaaattt ctgaacagcc ttaaaggtgg atctggaggt ggaggttctg
     1441 gaggagaaga acttttgagc aagaattatc atcttgagaa cgaagtggct cgtcttaaga
     1501 aaggttctgg cagtggagaa gaactgcttt caaagaatta ccacctggaa aatgaggtag
     1561 ctagactgaa aaaggggagc ggaagtgggg aggagttgct gagcaaaaat tatcatttgg
     1621 agaacgaagt agcacgacta aagaaagggt ccggatcggg tgaggagtta ctctcgaaaa
     1681 attatcatct cgaaaacgaa gtggctcggc taaaaaaggg cagtggttct ggataacccg
     1741 aacttcagac attttcccac agacttcctg gcctcctgtg actctggaaa gcaaaggatt
     1801 ggctgtgtat tgtccattga ttcctgattg acgccgtcaa aaacaaatgc ttgttaagcc
     1861 cataagcttt gcctgcttac tttctgccat tgggttggtt tgataccaca tttaacattg
     1921 acatttaagt ggaaaaccaa gttatcattg tcttttctaa gctcagtgtg gatgattgca
     1981 ttacttcatt cactgaagtt tttgcccaaa aattggaagg taaacagaga gctatgtttc
     2041 tgtatctttt ggttatagag tgttcacttc tttatcataa caaaattcta gtgtttatac
     2101 gaacacccag aggcaaaaga atttggctta attctcactc caggtaagta gcttaacttc
     2161 tgggcttcag ttttctcatc tgtaaaatca ggaagattgg actaagtgat cctgaaatgt
     2221 attttttagc actggatttc tacaaataat aaaactttcc catctagata atgatgatca
     2281 catagtcttg atgtacggac attaaaagcc agatttcttc attcaattct gttatctctg
     2341 ttttactctt tgaaattgat caagccactg aatcactttg catttcagtt tatatatata
     2401 gagagaaaga aggtgtctgc tcttacatta ttgtggagcc ctgtgataga aatatgtaaa
     2461 atctcatatt attttttttt taattttttt attttttatg acagggtctc actatgtcac
     2521 cctggctgga gtgcagtagt gcgatcgcgg cacactgcag ccttggcttc cctgggctca
     2581 agcagtcctc ccacctcagt ctcccaaata gctaggacta caggcgtgcg tgaccaagcc
     2641 cagctaattt ttgcattttt tgtagagatg gggttttgcc atgttgctca ggctggtctc
     2701 aaactcctga gcactagcaa tccacccacc tctatcccaa tggcgcgccg agcttggctc
     2761 gagcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     2821 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     2881 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     2941 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctgt tccgcttcct
     3001 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     3061 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     3121 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     3181 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     3241 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     3301 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     3361 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     3421 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     3481 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     3541 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     3601 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     3661 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     3721 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     3781 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     3841 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     3901 gtatatatga gtaaacttgg tctgacagtt agaaaaactc atcgagcatc aaatgaaact
     3961 gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg
     4021 aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga
     4081 ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat
     4141 caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca
     4201 tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat
     4261 caaccaaacc gttattcatt cgtgattgcg cctgagcgaa acgaaatacg cgatcgctgt
     4321 taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat
     4381 caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccag
     4441 ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg
     4501 gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg
     4561 caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc
     4621 gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat
     4681 cagcatccat gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc
     4741 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     4801 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     4861 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     4921 ggcgtatcac gaggcccttt tgtc
//
