LOCUS       40924_44873        3659 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning and transformation vector pUC-3GLA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Haussmann IU, Brauer U, Ustaoglu P, Hemani Y, Soller M.
  TITLE     Plasmid based gap-repair recombineering for Drosophila transgenesis 
            and the analysis of alternative pre-mRNA processing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Haussmann IU, Brauer U, Ustaoglu P, Hemani Y, Soller M.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2014) School of Bioscience, University of 
            Birmingham, Edgbaston, Birmingham, West Midlands B15 2TT, United 
            Kingdom
REFERENCE   3  (bases 1 to 3659)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3659)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-OCT-2014) School of Bioscience, University of Birmingham, 
            Edgbaston, Birmingham, West Midlands B15 2TT, United Kingdom"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(188..257)
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     promoter        625..729
                     /label=AmpR promoter
     CDS             730..1587
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1761..2349
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2370..2461
                     /label=multiple cloning site
                     /note="multiple cloning site"
     protein_bind    2466..2499
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    2504..2740
                     /label=3xP3-hsp70
                     /note="3xP3-hsp70"
     regulatory      2735..2744
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2756..3466
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    3503..3602
                     /note="ewg PA1; polyadenylation site 1 of the Drosophila
                     erect wing gene"
     protein_bind    complement(3614..3647)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_recomb     3641..3647
                     /label=loxP site for cre mediated recombination
                     /note="loxP site for cre mediated recombination"
ORIGIN
        1 tgcccgccgt gaccgtcgag aacccgctga cgctgccccg cgtatccgca cccgccgacg
       61 ccgtcgcacg tcccgtgctc accgtgacca ccgcgcccag cggtttcgag ggcgagggct
      121 tcccggtgcg ccgcgcgttc gccgggatca actaccgcca cctcgacccg ttcatcatga
      181 tggaccagat gggtgaggtg gagtacgcgc ccggggagcc caagggcacg ccctggcacc
      241 cgcaccgcgg cttcgagacc gtgacctaca tcgtcaccgg cttgacgacc ttggagcgac
      301 tgtcgttagt tccgcgcgat tcggtgcggt atttcacacc gcatatggtg cactctcagt
      361 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
      421 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
      481 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
      541 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
      601 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
      661 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
      721 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
      781 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
      841 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
      901 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
      961 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     1021 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     1081 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     1141 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     1201 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     1261 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     1321 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     1381 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     1441 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     1501 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     1561 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     1621 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     1681 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1741 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1801 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     1861 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     1921 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     1981 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     2041 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2101 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa
     2161 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2221 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     2281 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     2341 ctatggaaaa acgccttctt cttgaactcg aattctgtcg acagctgcag tactagtcaa
     2401 gcttaggatc cgatatccat ggtgctagcg agatcttcct cgagggtacc ggcggccgcg
     2461 tctagataac ttcgtataat gtatgctata cgaagttatc atgccgggga tctaattcaa
     2521 ttagagacta attcaattag agctaattca attaggatcc aagcttatcg atttcgaacc
     2581 ctcgaccgcc ggagtataaa tagaggcgct tcgtctacgg agcgacaatt caattcaaac
     2641 aagcaaagtg aacacgtcgc taagcgaaag ctaagcaaat aaacaagcgc agctgaacaa
     2701 gctaaacaat cgggcggccg tactagagcc ggtcgccacc atggtgtcga gcgcaagcaa
     2761 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     2821 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     2881 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     2941 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     3001 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     3061 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     3121 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     3181 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     3241 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     3301 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     3361 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     3421 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtact cagatctttg
     3481 caagcttgta gagtttccca tttaataatt catattatct cgaatctagt caattacggc
     3541 tttcctcaaa tagaaaaata aaaaaaatga aaaaatgcac ttgccattta aacttagacg
     3601 cgataagctt gatataactt cgtataatgt atgctatacg aagttatacg cgtactaga
//
