LOCUS       40924_44784       14835 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pUB-Cas9-@BAR, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14835)
  AUTHORS   Hahn F, Mantegazza O, Greiner A, Hegemann P, Eisenhut M, Weber AP.
  TITLE     An Efficient Visual Screen for CRISPR/Cas9 Activity in Arabidopsis 
            thaliana
  JOURNAL   Front Plant Sci 8, 39 (2017)
  PUBMED    28174584
REFERENCE   2  (bases 1 to 14835)
  AUTHORS   Hahn F, Mantegazza O, Eisenhut M, Greiner A, Hegemann P, Weber APM.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2016) Plant Biochemistry, Heinrich Heine 
            University Dusseldorf, Universitatsstr. 1, Dusseldorf 40225, Germany
REFERENCE   3  (bases 1 to 14835)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14835)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci 8, 39 (2017)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-NOV-2016) Plant Biochemistry, Heinrich Heine University 
            Dusseldorf, Universitatsstr. 1, Dusseldorf 40225, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14835
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    40..214
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    complement(292..316)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             741..1532
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      1622..2210
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(2396..2536)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2880..3074)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(3143..4207)
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4644..5270)
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     misc_feature    complement(6570..6594)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     6797..6813
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      6846..6855
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6850..6859
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        6903..7326
                     /label=AtU6-26
                     /note="Arabidopsis U6-26 gene promoter"
     misc_feature    7336..7356
                     /label=bar1 protospacer sequence
                     /note="bar1 protospacer sequence"
     misc_RNA        7357..7432
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     regulatory      7557..8196
                     /label=Arabidopsis thaliana Ubiquitin10 promoter
                     /note="Arabidopsis thaliana Ubiquitin10 promoter"
                     /regulatory_class="promoter"
     protein_bind    8236..8260
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8281..8301
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             8299..12402
                     /label=Cas9
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
     protein_bind    complement(12407..12431)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      12497..12744
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(12760..12776)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    12784..12800
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(12808..12838)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(12853..12874)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        13065..13742
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     CDS             13810..14832
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
ORIGIN
        1 agtagatgcc gaccggatct gtcgatcgac aagctcgagt ttctccataa taatgtgtga
       61 gtagttccca gataagggaa ttagggttcc tatagggttt cgctcatgtg ttgagcatat
      121 aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt
      181 cctaaaacca aaatccagta ctaaaatcca gatcccccga attaattcgg cgttaattca
      241 gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc
      301 acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc
      361 accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag
      421 gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg
      481 tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt
      541 gctgcctgtg atcaccgcgg tttcaaaatc ggctccgtcg atactatgtt atacgccaac
      601 tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa tgcaaggaac
      661 agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa atactgtaga
      721 aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg aaaaaactga
      781 tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag gtatataagc
      841 tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat aaagggacca
      901 cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag ctgcctgttc
      961 caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg agtgaggccg
     1021 atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag attatcgagc
     1081 tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt ccctatacga
     1141 atagcttaga cagccgctta gccgaattgg attacttact gaataacgat ctggccgatg
     1201 tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag ctgtatgatt
     1261 ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac ctgggagaca
     1321 gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg agaagcggca
     1381 gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag gatatcgggg
     1441 aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat tgggagaaaa
     1501 taaaatatta tattttactg gatgaattgt tttagtacct agaatgcatg accaaaatcc
     1561 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     1621 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1681 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     1741 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     1801 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     1861 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     1921 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     1981 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     2041 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     2101 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     2161 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     2221 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     2281 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     2341 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     2401 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
     2461 gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga
     2521 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     2581 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     2641 gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt gggcgccggc
     2701 ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag attgcctggc cgtaggccag
     2761 ccatttttga gcggccagcg gccgcgatag gccgacgcga agcggcgggg cgtagggagc
     2821 gcagcgaccg aagggtaggc gctttttgca gctcttcggc tgtgcgctgg ccagacagtt
     2881 atgcacaggc caggcgggtt ttaagagttt taataagttt taaagagttt taggcggaaa
     2941 aatcgccttt tttctctttt atatcagtca cttacatgtg tgaccggttc ccaatgtacg
     3001 gctttgggtt cccaatgtac gggttccggt tcccaatgta cggctttggg ttcccaatgt
     3061 acgtgctatc cacaggaaag agaccttttc gacctttttc ccctgctagg gcaatttgcc
     3121 ctagcatctg ctccgtacat taggaaccgg cggatgcttc gccctcgatc aggttgcggt
     3181 agcgcatgac taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg tactccggca
     3241 ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc
     3301 tgccactgcg ttcgtagatc gtcttgaaca accatctggc ttctgccttg cctgcggcgc
     3361 ggcgtgccag gcggtagaga aaacggccga tgccgggatc gatcaaaaag taatcggggt
     3421 gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc caatcagcta
     3481 gctcgatctc gatgtactcc ggccgcccgg tttcgctctt tacgatcttg tagcggctaa
     3541 tcaaggcttc accctcggat accgtcacca ggcggccgtt cttggccttc ttcgtacgct
     3601 gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg tctttctgct
     3661 ttccgccatc ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga cggaacacgc
     3721 ggccgggctt gtctcccttc ccttcccggt atcggttcat ggattcggtt agatgggaaa
     3781 ccgccatcag taccaggtcg taatcccaca cactggccat gccggccggc cctgcggaaa
     3841 cctctacgtg cccgtctgga agctcgtagc ggatcacctc gccagctcgt cggtcacgct
     3901 tcgacagacg gaaaacggcc acgtccatga tgctgcgact atcgcgggtg cccacgtcat
     3961 agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc ctaatcgacg
     4021 gcgcaccggc tgccggcggt tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc
     4081 acgattcacc ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct
     4141 tcaacttctc caccaggtca tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt
     4201 tgcgaccgtc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
     4261 acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
     4321 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
     4381 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
     4441 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
     4501 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
     4561 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
     4621 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
     4681 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
     4741 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
     4801 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
     4861 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
     4921 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
     4981 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
     5041 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
     5101 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
     5161 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
     5221 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
     5281 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
     5341 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
     5401 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
     5461 cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
     5521 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
     5581 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg
     5641 gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg
     5701 gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc
     5761 ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc
     5821 aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag
     5881 ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt
     5941 ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg
     6001 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
     6061 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
     6121 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
     6181 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
     6241 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
     6301 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
     6361 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
     6421 tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg
     6481 ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg
     6541 ttattctaat aaacgctctt ttctcttagg tttacccgcc aatatatcct gtcaaacact
     6601 gatagtttaa actgaaggcg ggaaacgaca atctgatcca agctcaagct gctctagcat
     6661 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     6721 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     6781 tcccagtcac gacgttgtaa aacgacggcc agtgccagaa ttgggcccga cgtcgcatgc
     6841 tcccggccgc catggcggcc gcgggaattc gattcacgaa gcttcgttga acaacggaaa
     6901 ctcgacttgc cttccgcaca atacatcatt tcttcttagc tttttttctt cttcttcgtt
     6961 catacagttt ttttttgttt atcagcttac attttcttga accgtagctt tcgttttctt
     7021 ctttttaact ttccattcgg agtttttgta tcttgtttca tagtttgtcc caggattaga
     7081 atgattaggc atcgaacctt caagaatttg attgaataaa acatcttcat tcttaagata
     7141 tgaagataat cttcaaaagg cccctgggaa tctgaaagaa gagaagcagg cccatttata
     7201 tgggaaagaa caatagtatt tcttatatag gcccatttaa gttgaaaaca atcttcaaaa
     7261 gtcccacatc gcttagataa gaaaacgaag ctgagtttat atacagctag agtcgaagta
     7321 gtgattgtcc cttcggcagg aaccgcagga gtaggagttt tagagctaga aatagcaagt
     7381 taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcaaattttt
     7441 ttaaactagt ggtgatcttg ctgaaaaact cgagccatcc ggaagatctt gaattcgcgg
     7501 ccgcctgcag gtcgaccata tgggagagct cccaacgcgt tggatgcata gcttggtacc
     7561 cgacgagtca gtaataaacg gcgtcaaagt ggttgcagcc ggcacacacg agtcgtgttt
     7621 atcaactcaa agcacaaata cttttcctca acctaaaaat aaggcaatta gccaaaaaca
     7681 actttgcgtg taaacaacgc tcaatacacg tgtcatttta ttattagcta ttgcttcacc
     7741 gccttagctt tctcgtgacc tagtcgtcct cgtcttttct tcttcttctt ctataaaaca
     7801 atacccaaag agctcttctt cttcacaatt cagatttcaa tttctcaaaa tcttaaaaac
     7861 tttctctcaa ttctctctac cgtgatcaag gtaaatttct gtgttcctta ttctctcaaa
     7921 atcttcgatt ttgttttcgt tcgatcccaa tttcgtatat gttctttggt ttagattctg
     7981 ttaatcttag atcgaagacg attttctggg tttgatcgtt agatatcatc ttaattctcg
     8041 attagggttt catagatatc atccgatttg ttcaaataat ttgagttttg tcgaataatt
     8101 actcttcgat ttgtgatttc tatctagatc tggtgttagt ttctagtttg tgcgatcgaa
     8161 tttgtagatt aatctgagtt tttctgatta acagctcgag tgcgggcccc ccctcgaggc
     8221 gcgccaagct atcaaacaag tttgtacaaa aaagcaggct accatatggc cagcagcccc
     8281 cccaagaaga agcgcaaggt cgacaagaag tactccatcg gcctggacat cggcacgaac
     8341 agcgtgggct gggccgtgat caccgacgag tacaaggtgc cgtccaagaa gttcaaggtg
     8401 ctgggcaaca ccgaccgcca cagcatcaag aagaacctga tcggcgccct gctgttcgac
     8461 tcgggcgaga ccgccgaggc gacgcgcctg aagcgcaccg cgcgccgccg ctacacgcgc
     8521 cgcaagaacc gcatctgcta cctgcaggag atcttcagca acgagatggc caaggtggac
     8581 gactcgttct tccaccgcct ggaggagtcc ttcctggtgg aggaggacaa gaagcacgag
     8641 cgccacccca tcttcggcaa catcgtggac gaggtggcct accacgagaa gtacccgacg
     8701 atctaccacc tgcgcaagaa gctggtggac agcaccgaca aggcggacct gcgcctgatc
     8761 tacctggccc tggcgcacat gatcaagttc cgcggccact tcctgatcga gggcgacctg
     8821 aaccccgaca actcggacgt ggacaagctg ttcatccagc tggtgcagac ctacaaccag
     8881 ctgttcgagg agaacccgat caacgcctcg ggcgtggacg ccaaggcgat cctgtccgcg
     8941 cgcctgtcca agagccgccg cctggagaac ctgatcgccc agctgcccgg cgagaagaag
     9001 aacggcctgt tcggcaacct gatcgcgctg agcctgggcc tgacgccgaa cttcaagtcg
     9061 aacttcgacc tggccgagga cgcgaagctg cagctgagca aggacaccta cgacgacgac
     9121 ctggacaacc tgctggccca gatcggcgac cagtacgcgg acctgttcct ggccgcgaag
     9181 aacctgtcgg acgccatcct gctgtccgac atcctgcgcg tgaacaccaa gatcacgaag
     9241 gcccccctgt cggcgtccat gatcaagcgc tacgacgagc accaccagga cctgaccctg
     9301 ctgaaggcgc tggtgcgcca gcagctgccg gagaagtaca aggagatctt cttcgaccag
     9361 tccaagaacg gctacgccgg ctacatcgac ggcggcgcca gccaggagga gttctacaag
     9421 ttcatcaagc ccatcctgga gaagatggac ggcacggagg agctgctggt gaagctgaac
     9481 cgcgaggacc tgctgcgcaa gcagcgcacc ttcgacaacg gcagcatccc ccaccagatc
     9541 cacctgggcg agctgcacgc catcctgcgc cgccaggagg acttctaccc gttcctgaag
     9601 gacaaccgcg agaagatcga gaagatcctg acgttccgca tcccctacta cgtgggcccg
     9661 ctggctcgcg gcaactcccg cttcgcgtgg atgacccgca agagcgagga gaccatcacg
     9721 ccctggaact tcgaggaggt ggtggacaag ggcgccagcg cgcagtcgtt catcgagcgc
     9781 atgaccaact tcgacaagaa cctgcccaac gagaaggtgc tgccgaagca ctccctgctg
     9841 tacgagtact tcaccgtgta caacgagctg acgaaggtga agtacgtgac cgagggcatg
     9901 cgcaagcccg ccttcctgag cggcgagcag aagaaggcga tcgtggacct gctgttcaag
     9961 accaaccgca aggtgacggt gaagcagctg aaggaggact acttcaagaa gatcgagtgc
    10021 ttcgacagcg tggagatctc gggcgtggag gaccgcttca acgccagcct gggcacctac
    10081 cacgacctgc tgaagatcat caaggacaag gacttcctgg acaacgagga gaacgaggac
    10141 atcctggagg acatcgtgct gaccctgacg ctgttcgagg accgcgagat gatcgaggag
    10201 cgcctgaaga cgtacgccca cctgttcgac gacaaggtga tgaagcagct gaagcgccgc
    10261 cgctacaccg gctggggccg cctgagccgc aagctgatca acggcatccg cgacaagcag
    10321 tcgggcaaga ccatcctgga cttcctgaag tccgacggct tcgcgaaccg caacttcatg
    10381 cagctgatcc acgacgactc gctgaccttc aaggaggaca tccagaaggc ccaggtgtcg
    10441 ggccagggcg actccctgca cgagcacatc gccaacctgg cgggctcccc cgcgatcaag
    10501 aagggcatcc tgcagaccgt gaaggtggtg gacgagctgg tgaaggtgat gggccgccac
    10561 aagccggaga acatcgtgat cgagatggcc cgcgagaacc agaccacgca gaagggccag
    10621 aagaacagcc gcgagcgcat gaagcgcatc gaggagggca tcaaggagct gggctcgcag
    10681 atcctgaagg agcaccccgt ggagaacacc cagctgcaga acgagaagct gtacctgtac
    10741 tacctgcaga acggccgcga catgtacgtg gaccaggagc tggacatcaa ccgcctgtcc
    10801 gactacgacg tggaccacat cgtgccccag agcttcctga aggacgactc gatcgacaac
    10861 aaggtgctga cccgcagcga caagaaccgc ggcaagagcg acaacgtgcc gtcggaggag
    10921 gtggtgaaga agatgaagaa ctactggcgc cagctgctga acgccaagct gatcacgcag
    10981 cgcaagttcg acaacctgac caaggccgag cgcggcggcc tgtcggagct ggacaaggcg
    11041 ggcttcatca agcgccagct ggtggagacc cgccagatca cgaagcacgt ggcgcagatc
    11101 ctggactccc gcatgaacac gaagtacgac gagaacgaca agctgatccg cgaggtgaag
    11161 gtgatcaccc tgaagtccaa gctggtcagc gacttccgca aggacttcca gttctacaag
    11221 gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcgt acctgaacgc cgtggtgggc
    11281 accgcgctga tcaagaagta ccccaagctg gagagcgagt tcgtgtacgg cgactacaag
    11341 gtgtacgacg tgcgcaagat gatcgccaag tcggagcagg agatcggcaa ggccaccgcg
    11401 aagtacttct tctactccaa catcatgaac ttcttcaaga ccgagatcac gctggccaac
    11461 ggcgagatcc gcaagcgccc cctgatcgag accaacggcg agacgggcga gatcgtgtgg
    11521 gacaagggcc gcgacttcgc gaccgtgcgc aaggtgctga gcatgcccca ggtgaacatc
    11581 gtgaagaaga ccgaggtgca gacgggcggc ttctccaagg agagcatcct gccgaagcgc
    11641 aactcggaca agctgatcgc ccgcaagaag gactgggacc ccaagaagta cggcggcttc
    11701 gactccccga ccgtggccta cagcgtgctg gtggtggcga aggtggagaa gggcaagtcc
    11761 aagaagctga agagcgtgaa ggagctgctg ggcatcacca tcatggagcg cagctcgttc
    11821 gagaagaacc ccatcgactt cctggaggcg aagggctaca aggaggtgaa gaaggacctg
    11881 atcatcaagc tgccgaagta ctcgctgttc gagctggaga acggccgcaa gcgcatgctg
    11941 gcctccgcgg gcgagctgca gaagggcaac gagctggccc tgcccagcaa gtacgtgaac
    12001 ttcctgtacc tggcgtccca ctacgagaag ctgaagggct cgccggagga caacgagcag
    12061 aagcagctgt tcgtggagca gcacaagcac tacctggacg agatcatcga gcagatctcg
    12121 gagttctcca agcgcgtgat cctggccgac gcgaacctgg acaaggtgct gagcgcctac
    12181 aacaagcacc gcgacaagcc catccgcgag caggcggaga acatcatcca cctgttcacc
    12241 ctgacgaacc tgggcgcccc ggccgcgttc aagtacttcg acaccacgat cgaccgcaag
    12301 cgctacacca gcacgaagga ggtgctggac gcgaccctga tccaccagag catcaccggc
    12361 ctgtacgaga cgcgcatcga cctgtcgcag ctgggcggcg actaataccc agctttcttg
    12421 tacaaagtgg ttcgataatt ccttaattaa ctagttctag agcggccgcc caccgcggtg
    12481 gagctcgaat ttccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc
    12541 tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat
    12601 aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca
    12661 attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc
    12721 gcgcgcggtg tcatctatgt tactgaattc gtaatcatgg tcatagctgt ttcctgtgtg
    12781 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
    12841 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
    12901 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
    12961 cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac
    13021 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
    13081 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa
    13141 aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct
    13201 atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
    13261 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac
    13321 atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac
    13381 caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat
    13441 tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa
    13501 tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc
    13561 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct
    13621 tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac
    13681 tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct
    13741 gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg
    13801 caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa
    13861 aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc
    13921 agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc
    13981 tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg
    14041 cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt
    14101 gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag
    14161 gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga
    14221 ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc
    14281 catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct
    14341 ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg
    14401 gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg
    14461 agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg
    14521 tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca
    14581 ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc
    14641 ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc
    14701 cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg
    14761 accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg
    14821 agggcaaaga aatag
//
