LOCUS       40924_44694       10448 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUASg-HA.attB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10448)
  AUTHORS   Bischof J, Bjorklund M, Furger E, Schertel C, Taipale J, Basler K.
  TITLE     A versatile platform for creating a comprehensive UAS-ORFeome 
            library in Drosophila
  JOURNAL   Development 140 (11), 2434-2442 (2013)
  PUBMED    23637332
REFERENCE   2  (bases 1 to 10448)
  AUTHORS   Bischof J, Bjorklund M, Furger E, Schertel C, Taipale J, Basler K.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-APR-2013) Institute of Molecular Life Sciences, 
            University of Zurich, Winterthurerstrasse 190, Zurich 8057, 
            Switzerland
REFERENCE   3  (bases 1 to 10448)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10448)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development"; date: "2013"; volume: "140"; issue: "11"; pages: 
            "2434-2442"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-APR-2013) Institute of Molecular Life Sciences, University of 
            Zurich, Winterthurerstrasse 190, Zurich 8057, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..10448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            666..4802
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     protein_bind    4842..4936
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        4955..5193
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     protein_bind    5230..5354
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        5379..5409
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             5463..6140
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             6463..6765
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(6809..6933)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      6941..6950
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6980..7069
                     /label=3xHA
                     /note="three tandem HA epitope tags"
     regulatory      7108..7920
                     /label=tubulin alpha 1 terminator
                     /note="tubulin alpha 1 terminator"
                     /regulatory_class="terminator"
     protein_bind    7972..8041
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     promoter        complement(8260..8278)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8299..8315)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8323..8339
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8347..8377)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8392..8413)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8701..9289)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9463..10320)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(10321..10425)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgctattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatttggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagtta gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaaaat aaataaattt catttaactc gcgaacatgt tgaagatatg
     3181 aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct
     3241 catctttttg ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg gagccatctt
     3301 cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcttgt
     3361 ttagaataca tttgcatatt aataatttac taactttcta atgaatcgat tcgatttagg
     3421 tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg
     3481 acacatactt tctgggcaaa acgattgccg aattaccgct ttttctcaca gtgccactgg
     3541 tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca
     3601 actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat
     3661 cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat
     3721 tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     3781 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     3841 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     3901 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     3961 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     4021 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta
     4081 ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc
     4141 aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag
     4201 tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa
     4261 actcgtttat cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc
     4321 gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt
     4381 ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg
     4441 ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag
     4501 aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt
     4561 ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga
     4621 cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct
     4681 tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa
     4741 taacaactat aataataaga atacatttaa tttagaaaat gcttggattt cactggaact
     4801 aggctagagc ttaggattta aatgcttgca tgcctgcagg tcggagtact gtcctccgag
     4861 cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg
     4921 agtactgtcc tccgagcgga gactctagcg agcgccggag tataaataga ggcgcttcgt
     4981 ctacggagcg acaattcaat tcaaacaagc aaagtgaaca cgtcgctaag cgaaagctaa
     5041 gcaaataaac aagcgcagct gaacaagcta aacaatctgc agtaaagtgc aagttaaagt
     5101 gaatcaatta aaagtaacca gcaaccaagt aaatcaactg caactactga aatctgccaa
     5161 gaagtaatta ttgaatacaa gaagagaact ctgaataggg aattgggaat tcgttaacag
     5221 atctgatcga caagtttgta caaaaaagct gaacgagaaa cgtaaaatga tataaatatc
     5281 aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat
     5341 atccagtcac tatggcggcc gcattaggca ccccaggctt tacactttat gcttccggct
     5401 cgtataatgt gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta
     5461 aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag
     5521 aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg
     5581 atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta
     5641 ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg
     5701 gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg
     5761 aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat
     5821 attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg
     5881 agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg
     5941 tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag
     6001 gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc
     6061 atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag gcggggcgta
     6121 atctagagga tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt
     6181 ttgcggtata agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct
     6241 atgaagcagc gtattacagt gacagttgac accgacagct atcagttgct caaggcatat
     6301 atgatgtcaa tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc
     6361 gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg
     6421 aaatgaacgg ctcttttgct gacgagaaca ggggctggtg aaatgcagtt taaggtttac
     6481 acctataaaa gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac
     6541 acgcccgggc gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc
     6601 ccccgtgaac tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc
     6661 gatatggcca gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc
     6721 gaaaatgaca tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc
     6781 cttatacaca gccagtctgc aggtcgacca tagtgactgg atatgttgtg ttttacagta
     6841 ttatgtagtc tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta
     6901 cgtttctcgt tcagctttct tgtacaaagt ggtgagctcc gccaccatgg atctccaccg
     6961 cggtggaggc cgcatctttt acccatacga tgttcctgac tatgcgggct atccctatga
     7021 cgtcccggac tatgcaggat cctatccata tgacgttcca gattacgctg ctcatggcgg
     7081 acaccggtcc acgtgacgta agctagcact aagcgtcgcg ccacttcaac gctcgatggg
     7141 agcgtcattg gtgggcgggg taaccgtcga aatcagtgtt tacgcttcca atcgcaacaa
     7201 aaaattcact gcaacactga aaagcatacg aaaacgatga agattgtacg agaaaccata
     7261 aagtatttta tccacaaaga cacgtatagc agaaaagcca agttaactcg gcgataagtt
     7321 gtgtacacaa gaataaaatc ggccagattc agtgttgtca gaaataagaa aaccccacta
     7381 tgtttttctt tgccttttct ttctcccagc gatcattcat ttcgtggtga aagaacgggg
     7441 tcattgcacg gagtttcgac tgcgggaaag cagagctgcc gttcacttcg tctataatta
     7501 gcgctttcta ttttccccga ttcgggccgc tgctgcgctt ttccgcctgc tgtttgtggc
     7561 aagtgtagca gcaggctgtg cacgcagtgt ggcatgcact tggctttcca ccgttggtat
     7621 cgattctctg ggacgatgag tcattccttt cggggccaca gcataatcgt tgccagctca
     7681 ccgaaatggt gacttcattt cttaactgcc gtcaagcatg cgattgtaca tacatacata
     7741 tttatatatg tacatattta tgtgactatg gtaggtcgat ataatagcaa tcaacgcaag
     7801 caaatgtgtc agtcctgctt acaggaacga ttctatttag taattttcgt tgtataaagt
     7861 aattatgtat gtatgtaagc cccataaatc tgaaacaatt aggcaaaacc atgcgaagct
     7921 ctcgagctcg gtaagcttag atccactagt gtcgacgatg taggtcacgg tctcgaagcc
     7981 gcggtgcggg tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat
     8041 ctggtccatc atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg
     8101 caccgggaag ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg
     8161 tgcgacggcg tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac
     8221 ggcgggcatg tcgacactag ttctagccag cttttgttcc ctttagtgag ggttaatttc
     8281 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     8341 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     8401 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     8461 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     8521 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     8581 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     8641 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     8701 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     8761 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     8821 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     8881 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     8941 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     9001 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     9061 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     9121 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     9181 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     9241 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     9301 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     9361 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     9421 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     9481 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     9541 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     9601 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     9661 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     9721 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     9781 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     9841 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     9901 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     9961 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    10021 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    10081 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    10141 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    10201 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    10261 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    10321 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    10381 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    10441 agtgccac
//
