LOCUS       V002462                10106 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002462
VERSION     V002462
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10106)
  AUTHORS   Southall TD, Gold KS, Egger B, Davidson CM, Caygill EE, Marshall OJ,
            Brand AH.
  TITLE     Cell-type-specific profiling of gene expression and chromatin
            binding without cell isolation: assaying RNA Pol II occupancy in
            neural stem cells
  JOURNAL   Dev. Cell 26 (1), 101-112 (2013)
   PUBMED   23792147
REFERENCE   2  (bases 1 to 10106)
  AUTHORS   Southall TD, Brand AH.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2016) Life Sciences, Imperial College London, Sir
            Ernst Chain Building, London SW7 2AZ, United Kingdom
REFERENCE   3  (bases 1 to 10106)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10106)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Dev. Cell";
            date: "2013"; volume: "26"; issue: "1"; pages: "101-112"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-FEB-2016) Life Sciences, Imperial College London, Sir Ernst
            Chain Building, London SW7 2AZ, United Kingdom"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        623..641
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            666..4802
                     /label="mini-white"
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     protein_bind    4809..4842
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4875..4969
                     /label="5X UAS"
                     /note="five tandem copies of the 'ScaI site' 17-mer
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS)
                     that efficiently binds yeast Gal4 (Webster et al., 1988;
                     Pfeiffer et al., 2010)"
     promoter        4988..5226
                     /label="hsp70 promoter"
                     /note="Drosophila melanogaster hsp70Bb promoter"
     CDS             5261..5968
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     CDS             5991..6824
                     /gene="dam"
                     /label="DNA adenine methylase"
                     /note="DNA adenine methylase from Escherichia coli (strain
                     K12). Accession#: P0AEE8"
     CDS             6831..6860
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     intron          6980..7045
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7175..7195
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    7467..7601
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     protein_bind    7630..7699
                     /label="attB"
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     promoter        complement(7918..7936)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7957..7973)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    7981..7997
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8005..8035)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8050..8071)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8359..8947)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9121..9978)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9979..10083)
                     /label="AmpR promoter"
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgctattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatttggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagtta gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaaaat aaataaattt catttaactc gcgaacatgt tgaagatatg
     3181 aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct
     3241 catctttttg ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg gagccatctt
     3301 cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcttgt
     3361 ttagaataca tttgcatatt aataatttac taactttcta atgaatcgat tcgatttagg
     3421 tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg
     3481 acacatactt tctgggcaaa acgattgccg aattaccgct ttttctcaca gtgccactgg
     3541 tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca
     3601 actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat
     3661 cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat
     3721 tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     3781 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     3841 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     3901 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     3961 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     4021 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta
     4081 ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc
     4141 aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag
     4201 tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa
     4261 actcgtttat cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc
     4321 gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt
     4381 ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg
     4441 ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag
     4501 aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt
     4561 ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga
     4621 cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct
     4681 tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa
     4741 taacaactat aataataaga atacatttaa tttagaaaat gcttggattt cactggaact
     4801 aggctagcat aacttcgtat aatgtatgct atacgaagtt atgctagcgg atccaagctt
     4861 gcatgcctgc aggtcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac
     4921 tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc ggagactcta
     4981 gcgagcgccg gagtataaat agaggcgctt cgtctacgga gcgacaattc aattcaaaca
     5041 agcaaagtga acacgtcgct aagcgaaagc taagcaaata aacaagcgca gctgaacaag
     5101 ctaaacaatc tgcagtaaag tgcaagttaa agtgaatcaa ttaaaagtaa ccagcaacca
     5161 agtaaatcaa ctgcaactac tgaaatctgc caagaagtaa ttattgaata caagaagaga
     5221 actctgaata gggaattggg aattcatggc aactagcggc atggttagta aaggagaaga
     5281 aaataacatg gcaatcatta aggagttcat gagattcaaa gttcacatgg aaggttctgt
     5341 aaatggacat gaatttgaaa tagaaggtga aggagaagga aggccttatg aaggaaccca
     5401 aaccgcgaag ctaaaagtta ctaagggtgg cccattacca tttgcatggg atatccttag
     5461 ccctcaattc atgtatgggt caaaggctta tgtcaagcac cccgccgaca ttccagacta
     5521 tctaaagtta tcttttcccg aagggtttaa gtgggagcgt gtgatgaact tcgaagacgg
     5581 tggcgtggta acagtgactc aggattcgtc cctgcaagat ggtgaattta tctacaaagt
     5641 caaattaaga ggaactaact ttccatctga cggcccggtt atgcaaaaaa agacaatggg
     5701 ctgggaggcc tcctcagaac gaatgtaccc tgaagatggt gccttgaagg gtgagattaa
     5761 acaaagattg aaattgaaag atggtggaca ttatgacgct gaggttaaaa cgacatacaa
     5821 agctaagaaa cctgtccagc tcccaggtgc ttacaatgta aatataaaac ttgatattac
     5881 atcacataat gaagattata cgatagttga acaatacgaa agggctgagg ggagacatag
     5941 tactggtggc atggatgaac tatacaaagg ttctggtacc gcataataac atgaagaaaa
     6001 atcgcgcttt tttgaagtgg gcagggggca agtatcccct gcttgatgat attaaacggc
     6061 atttgcccaa gggcgaatgt ctggttgagc cttttgtagg tgccgggtcg gtgtttctca
     6121 acaccgactt ttctcgttac atccttgccg atatcaatag cgacctgatc agtctctata
     6181 acattgtgaa gatgcgtact gatgagtacg tacaggccgc acgcgagctg tttgttcccg
     6241 aaacaaattg cgccgaggtt tactatcagt tccgcgaaga gttcaacaaa agccaggatc
     6301 cgttccgtcg ggcggtactg tttttatatt tgaaccgcta cggttacaac ggcctgtgtc
     6361 gttacaatct gcgcggtgag tttaacgtgc cgttcggccg ctacaaaaaa ccctatttcc
     6421 cggaagcaga gttgtatcac ttcgctgaaa aagcgcagaa tgcctttttc tattgtgagt
     6481 cttacgccga tagcatggcg cgcgcagatg atgcatccgt cgtctattgc gatccgcctt
     6541 atgcaccgct gtctgcgacc gccaacttta cggcgtatca cacaaacagt tttacgcttg
     6601 aacaacaagc gcatctggcg gagatcgccg aaggtctggt tgagcgccat attccagtgc
     6661 tgatctccaa tcacgatacg atgttaacgc gtgagtggta tcagcgcgca aaattgcatg
     6721 tcgtcaaagt tcgacgcagt ataagcagca acggcggcac acgtaaaaag gtggacgaac
     6781 tgctggcttt gtacaaacca ggagtcgttt cacccgcgaa aaaagccggt gaacagaaac
     6841 tcatctctga agaggatctg gccggcgcag atctgcggcc gcggctcgag ggtacctcta
     6901 gaggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac
     6961 agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact
     7021 gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg
     7081 gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga
     7141 tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga
     7201 ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag
     7261 aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa
     7321 aattatggaa aaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca
     7381 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     7441 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     7501 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     7561 gtttgtccaa actcatcaat gtatcttatc atgtctggat ccactagtgt cgacgatgta
     7621 ggtcacggtc tcgaagccgc ggtgcgggtg ccagggcgtg cccttgggct ccccgggcgc
     7681 gtactccacc tcacccatct ggtccatcat gatgaacggg tcgaggtggc ggtagttgat
     7741 cccggcgaac gcgcggcgca ccgggaagcc ctcgccctcg aaaccgctgg gcgcggtggt
     7801 cacggtgagc acgggacgtg cgacggcgtc ggcgggtgcg gatacgcggg gcagcgtcag
     7861 cgggttctcg acggtcacgg cgggcatgtc gacactagtt ctagccagct tttgttccct
     7921 ttagtgaggg ttaatttcga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     7981 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     8041 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     8101 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     8161 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8221 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8281 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8341 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8401 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8461 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8521 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     8581 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8641 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8701 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8761 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     8821 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8881 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     8941 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9001 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9061 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9121 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9181 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9241 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9301 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9361 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9421 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9481 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9541 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9601 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9661 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     9721 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     9781 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     9841 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     9901 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     9961 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    10021 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10081 gcgcacattt ccccgaaaag tgccac
//
