LOCUS       40924_44744        9627 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUAST-CTAP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9627)
  AUTHORS   Veraksa A, Bauer A, Artavanis-Tsakonas S.
  TITLE     Analyzing protein complexes in Drosophila with tandem affinity 
            purification-mass spectrometry
  JOURNAL   Dev. Dyn. 232 (3), 827-834 (2005)
  PUBMED    15704125
REFERENCE   2  (bases 1 to 9627)
  AUTHORS   Veraksa A, Bauer A, Artavanis-Tsakonas S.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-AUG-2004) MGH Cancer Center, Harvard Medical School, 
            Bldg 149, 13th Street, Charlestown, MA 02129, USA
REFERENCE   3  (bases 1 to 9627)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9627)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev. Dyn.";
            date: "2005"; volume: "232"; issue: "3"; pages: "827-834"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-AUG-2004) MGH Cancer Center, Harvard Medical School, Bldg 149, 
            13th Street, Charlestown, MA 02129, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9627
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(997..1854)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1855..1959)
                     /label=AmpR promoter
     misc_feature    complement(2761..2993)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    3055..3149
                     /label=5X UAS
                     /note="five tandem copies of the 'ScaI site' 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3168..3406
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     misc_feature    3420..3465
                     /note="polylinker; unique cloning sites for EcoRI,
                     EagI/NotI, XhoI, KpnI, XbaI; XbaI site is dam methylation 
                     sensitive"
     CDS             3475..3552
                     /label=CBP
                     /note="calmodulin-binding peptide"
     CDS             3580..3600
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     CDS             3640..3813
                     /label=ProtA
                     /note="IgG-binding unit of Staphylococcus aureus protein A"
     CDS             3817..3987
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="DNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLA
                     EAKKLNGAQAPK"
     intron          4103..4168
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4298..4318
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    4743..4877
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     gene            4895..9031
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     misc_feature    complement(9042..9627)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg
     2281 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2341 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2401 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2461 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2521 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2581 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2641 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     2701 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     2761 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
     2821 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
     2881 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
     2941 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
     3001 gctgcatcca acgcgttggg agctctccgg atccaagctt gcatgcctgc aggtcggagt
     3061 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
     3121 gtcctccgag cggagtactg tcctccgagc ggagactcta gcgagcgccg gagtataaat
     3181 agaggcgctt cgtctacgga gcgacaattc aattcaaaca agcaaagtga acacgtcgct
     3241 aagcgaaagc taagcaaata aacaagcgca gctgaacaag ctaaacaatc tgcagtaaag
     3301 tgcaagttaa agtgaatcaa ttaaaagtaa ccagcaacca agtaaatcaa ctgcaactac
     3361 tgaaatctgc caagaagtaa ttattgaata caagaagaga actctgaata gggaattggg
     3421 aattcgttaa cagatctgcg gccgcggctc gagggtacct ctagatccat ggaaaagaga
     3481 agatggaaaa agaatttcat agccgtctca gcagccaacc gctttaagaa aatctcatcc
     3541 tccggggcac ttgattatga tattccaact actgctagcg agaatttgta ttttcagggt
     3601 gagctcaaaa ccgcggctct tgcgcaacac gatgaagccg tggacaacaa attcaacaaa
     3661 gaacaacaaa acgcgttcta tgagatctta catttaccta acttaaacga agaacaacga
     3721 aacgccttca tccaaagttt aaaagatgac ccaagccaaa gcgctaacct tttagcagaa
     3781 gctaaaaagc taaatgatgc tcaggcgccg aaagtagaca acaaattcaa caaagaacaa
     3841 caaaacgcgt tctatgagat cttacattta cctaacttaa acgaagaaca acgaaacgcc
     3901 ttcatccaaa gtttaaaaga tgacccaagc caaagcgcta accttttagc agaagctaaa
     3961 aagctaaatg gtgctcaggc gccgaaagta gacgcgaact ccgcggggaa gtcaacctga
     4021 ctagaggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
     4081 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
     4141 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
     4201 gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga
     4261 tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga
     4321 agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa
     4381 tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa
     4441 gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa
     4501 catactgttt tttcttactc cacacaggca tagagtgtct gctattaata actatgctca
     4561 aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta
     4621 tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     4681 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     4741 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     4801 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     4861 cttatcatgt ctggatcgga tccactagaa ggccttagta tgtatgtaag ttaataaaac
     4921 ccttttttgg agaatgtaga tttaaaaaaa catatttttt ttttattttt tactgcactg
     4981 gacatcattg aacttatctg atcagtttta aatttacttc gatccaaggg tatttgaagt
     5041 accaggttct ttcgattacc tctcactcaa aatgacattc cactcaaagt cagcgctgtt
     5101 tgcctccttc tctgtccaca gaaatatcgc cgtctctttc gccgctgcgt ccgctatctc
     5161 tttcgccacc gtttgtagcg ttacctagcg tcaatgtccg ccttcagttg cactttgtca
     5221 gcggtttcgt gacgaagctc caagcggttt acgccatcaa ttaaacacaa agtgctgtgc
     5281 caaaactcct ctcgcttctt atttttgttt gttttttgag tgattggggt ggtgattggt
     5341 tttgggtggg taagcagggg aaagtgtgaa aaatcccggc aatgggccaa gaggatcagg
     5401 agctattaat tcgcggaggc agcaaacacc catctgccga gcatctgaac aatgtgagta
     5461 gtacatgtgc atacatctta agttcacttg atctatagga actgcgattg caacatcaaa
     5521 ttgtctgcgg cgtgagaact gcgacccaca aaaatcccaa accgcaatcg cacaaacaaa
     5581 tagtgacacg aaacagatta ttctggtagc tgtgctcgct atataagaca atttttaaga
     5641 tcatatcatg atcaagacat ctaaaggcat tcattttcga ctacattctt ttttacaaaa
     5701 aatataacaa ccagatattt taagctgatc ctagatgcac aaaaaataaa taaaagtata
     5761 aacctacttc gtaggatact tcgttttgtt cggggttaga tgagcataac gcttgtagtt
     5821 gatatttgag atcccctatc attgcagggt gacagcggag cggcttcgca gagctgcatt
     5881 aaccagggct tcgggcaggc caaaaactac ggcacgctcc tgccacccag tccgccggag
     5941 gactccggtt cagggagcgg ccaactagcc gagaacctca cctatgcctg gcacaatatg
     6001 gacatctttg gggcggtcaa tcagccgggc tccggatggc ggcagctggt caaccggaca
     6061 cgcggactat tctgcaacga gcgacacata ccggcgccca ggaaacattt gctcaagaac
     6121 ggtgagtttc tattcgcagt cggctgatct gtgtgaaatc ttaataaagg gtccaattac
     6181 caatttgaaa ctcagtttgc ggcgtggcct atccgggcga acttttggcc gtgatgggca
     6241 gttccggtgc cggaaagacg accctgctga atgcccttgc ctttcgatcg ccgcagggca
     6301 tccaagtatc gccatccggg atgcgactgc tcaatggcca acctgtggac gccaaggaga
     6361 tgcaggccag gtgcgcctat gtccagcagg atgacctctt tatcggctcc ctaacggcca
     6421 gggaacacct gattttccag gccatggtgc ggatgccacg acatctgacc tatcggcagc
     6481 gagtggcccg cgtggatcag gtgatccagg agctttcgct cagcaaatgt cagcacacga
     6541 tcatcggtgt gcccggcagg gtgaaaggtc tgtccggcgg agaaaggaag cgtctggcat
     6601 tcgcctccga ggcactaacc gatccgccgc ttctgatctg cgatgagccc acctccggac
     6661 tggactcatt taccgcccac agcgtcgtcc aggtgctgaa gaagctgtcg cagaagggca
     6721 agaccgtcat cctgaccatt catcagccgt cttccgagct gtttgagctc tttgacaaga
     6781 tccttctgat ggccgagggc agggtagctt tcttgggcac tcccagcgaa gccgtcgact
     6841 tcttttccta gtgagttcga tgtgtttatt aagggtatct agcattacat tacatctcaa
     6901 ctcctatcca gcgtgggtgc ccagtgtcct accaactaca atccggcgga cttttacgta
     6961 caggtgttgg ccgttgtgcc cggacgggag atcgagtccc gtgatcggat cgccaagata
     7021 tgcgacaatt ttgctattag caaagtagcc cgggatatgg agcagttgtt ggccaccaaa
     7081 aatttggaga agccactgga gcagccggag aatgggtaca cctacaaggc cacctggttc
     7141 atgcagttcc gggcggtcct gtggcgatcc tggctgtcgg tgctcaagga accactcctc
     7201 gtaaaagtgc gacttattca gacaacggtg agtggttcca gtggaaacaa atgatataac
     7261 gcttacaatt cttggaaaca aattcgctag attttagtta gaattgcctg attccacacc
     7321 cttcttagtt tttttcaatg agatgtatag tttatagttt tgcagaaaat aaataaattt
     7381 catttaactc gcgaacatgt tgaagatatg aatattaatg agatgcgagt aacattttaa
     7441 tttgcagatg gttgccatct tgattggcct catctttttg ggccaacaac tcacgcaagt
     7501 gggcgtgatg aatatcaacg gagccatctt cctcttcctg accaacatga cctttcaaaa
     7561 cgtctttgcc acgataaatg taagtcttgt ttagaataca tttgcatatt aataatttac
     7621 taactttcta atgaatcgat tcgatttagg tgttcacctc agagctgcca gtttttatga
     7681 gggaggcccg aagtcgactt tatcgctgtg acacatactt tctgggcaaa acgattgccg
     7741 aattaccgct ttttctcaca gtgccactgg tcttcacggc gattgcctat ccgatgatcg
     7801 gactgcgggc cggagtgctg cacttcttca actgcctggc gctggtcact ctggtggcca
     7861 atgtgtcaac gtccttcgga tatctaatat cctgcgccag ctcctcgacc tcgatggcgc
     7921 tgtctgtggg tccgccggtt atcataccat tcctgctctt tggcggcttc ttcttgaact
     7981 cgggctcggt gccagtatac ctcaaatggt tgtcgtacct ctcatggttc cgttacgcca
     8041 acgagggtct gctgattaac caatgggcgg acgtggagcc gggcgaaatt agctgcacat
     8101 cgtcgaacac cacgtgcccc agttcgggca aggtcatcct ggagacgctt aacttctccg
     8161 ccgccgatct gccgctggac tacgtgggtc tggccattct catcgtgagc ttccgggtgc
     8221 tcgcatatct ggctctaaga cttcgggccc gacgcaagga gtagccgaca tatatccgaa
     8281 ataactgctt gttttttttt ttaccattat taccatcgtg tttactgttt attgccccct
     8341 caaaaagcta atgtaattat atttgtgcca ataaaaacaa gatatgacct atagaataca
     8401 agtatttccc cttcgaacat ccccacaagt agactttgga tttgtcttct aaccaaaaga
     8461 cttacacacc tgcatacctt acatcaaaaa ctcgtttatc gctacataaa acaccgggat
     8521 atatttttta tatacatact tttcaaatcg cgcgccctct tcataattca cctccaccac
     8581 accacgtttc gtagttgctc tttcgctgtc tcccacccgc tctccgcaac acattcacct
     8641 tttgttcgac gaccttggag cgactgtcgt tagttccgcg cgattcggtt cgctcaaatg
     8701 gttccgagtg gttcatttcg tctcaataga aattagtaat aaatatttgt atgtacaatt
     8761 tatttgctcc aatatatttg tatatatttc cctcacagct atatttattc taatttaata
     8821 ttatgacttt ttaaggtaat tttttgtgac ctgttcggag tgattagcgt tacaatttga
     8881 actgaaagtg acatccagtg tttgttcctt gtgtagatgc atctcaaaaa aatggtgggc
     8941 ataatagtgt tgtttatata tatcaaaaat aacaactata ataataagaa tacatttaat
     9001 ttagaaaatg cttggatttc actggaacta gaattaattc ggctgctgct ctaaacgacg
     9061 catttcgtac tccaaagtac gaattttttc cctcaagctc ttattttcat taaacaatga
     9121 acaggaccta acgcacagtc acgttattgt ttacataaat gatttttttt actattcaaa
     9181 cttactctgt ttgtgtactc ccactggtat agccttcttt tatcttttct ggttcaggct
     9241 ctatcacttt actaggtacg gcatctgcgt tgagtcgcct ccttttaaat gtctgacctt
     9301 ttgcaggtgc agccttccac tgcgaatcat taaagtgggt atcacaaatt tgggagtttt
     9361 caccaaggct gcacccaagg ctctgctccc acaattttct cttaatagca cacttcggca
     9421 cgtgaattaa ttttactcca gtcacagctt tgcagcaaaa tttgcaatat ttcatttttt
     9481 tttattccac gtaagggtta atgttttcaa aaaaaaattc gtccgcacac aacctttcct
     9541 ctcaacaagc aaacgtgcac tgaatttaag tgtatacttc ggtaagcttc ggctatcgac
     9601 gggaccacct tatgttattt catcatg
//
