LOCUS       62056_21780        4260 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1013..1726
                     /codon_start=1
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFAYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      1767..1853
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(2394..3341)
                     /codon_start=1
                     /label=Rep101
                     /note="RepA protein needed for replication with the pSC101 
                     origin"
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      complement(3389..3611)
                     /direction=LEFT
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(4103..4197)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(4231..4260,1..762))
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc
       61 gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc
      121 cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt
      181 cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgcgcgcctt
      241 gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg
      301 atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg
      361 gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat
      421 ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc
      481 caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac
      541 gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc tgcagttcat tcagggcacc
      601 ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc
      661 ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca
      721 agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc
      781 tgtctcttga tcagatcttg atcccctgcg ccatcagatc cttggcggca agaaagccat
      841 ccagtttact ttgcagggct tcccaacctt accagagggc gccccagctg gcaattccga
      901 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
      961 ctttcgtctt cacctcgagg ggatcctcta gatttaagaa ggagatatac atatgagtaa
     1021 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa
     1081 tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac
     1141 ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac
     1201 tttcgcgtat ggtcttcaat gctttgcgag atacccagat catatgaaac agcatgactt
     1261 tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga
     1321 cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat
     1381 cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata
     1441 caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt
     1501 taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag accattatca
     1561 acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac
     1621 acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt
     1681 tgtaacagct gctgggatta cccatggtat ggatgaattg tacaaataaa tgtccagacc
     1741 tgcaggcatg caagctctag aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     1801 cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgc
     1861 cctagaccta gggtacgggt tttgctgccc gcaaacgggc tgttctggtg ttgctagttt
     1921 gttatcagaa tcgcagatcc ggcttcagcc ggtttgccgg ctgaaagcgc tatttcttcc
     1981 agaattgcca tgattttttc cccacgggag gcgtcactgg ctcccgtgtt gtcggcagct
     2041 ttgattcgat aagcagcatc gcctgtttca ggctgtctat gtgtgactgt tgagctgtaa
     2101 caagttgtct caggtgttca atttcatgtt ctagttgctt tgttttactg gtttcacctg
     2161 ttctattagg tgttacatgc tgttcatctg ttacattgtc gatctgttca tggtgaacag
     2221 ctttgaatgc accaaaaact cgtaaaagct ctgatgtatc tatctttttt acaccgtttt
     2281 catctgtgca tatggacagt tttccctttg atatgtaacg gtgaacagtt gttctacttt
     2341 tgtttgttag tcttgatgct tcactgatag atacaagagc cataagaacc tcagatcctt
     2401 ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag ccatgagaac
     2461 gaaccattga gatcatactt actttgcatg tcactcaaaa attttgcctc aaaactggtg
     2521 agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt atgtaggtag
     2581 gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg gttgttctca
     2641 agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa cgtatcagtc
     2701 gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa atctttactt
     2761 attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt ttcaagcatt
     2821 aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt tttcttttgt
     2881 gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc aaaagactta
     2941 acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg caatatctct
     3001 tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt ccactggaaa
     3061 atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat cagctctctg
     3121 gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat ctgagcgtat
     3181 tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat cgtggggttg
     3241 agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc atagcgacta
     3301 atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa ttggtctagg
     3361 tgattttaat cactatacca attgagatgg gctagtcaat gataattact agtccttttc
     3421 ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact tgtaaattct
     3481 gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta tattcaagtg
     3541 gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga tcccagccct
     3601 gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc gcaaacgctg
     3661 tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac cctcgcaagc
     3721 tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga gtcgctgtct
     3781 ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg gtaaatggca
     3841 ctacaggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag aaaagcccgt
     3901 cacgggcttc tcagggcgtt ttatggcggg tctgctatgt ggtgctatct gactttttgc
     3961 tgttcagcag ttcctgccct ctgattttcc agtctgacca cttcggatta tcccgtgaca
     4021 ggtcattcag actggctaat gcacccagta aggcagcggt atcatcaaca ggcttacccg
     4081 tcttactgtc cctagtgctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg
     4141 ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa
     4201 gcgagctctc gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc
//
