LOCUS       40924_44669       13415 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUA-Os, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13415)
  AUTHORS   Zhou J, Yu F, Chen B, Wang X, Yang Y, Cheng Y, Yan C, Chen J.
  TITLE     Universal vectors for constructing artificial microRNAs in plants
  JOURNAL   Biotechnol. Lett. 35 (7), 1127-1133 (2013)
  PUBMED    23568377
REFERENCE   2  (bases 1 to 13415)
  AUTHORS   Zhou J, Wang XM, Yan CQ, Chen JP.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-OCT-2012) Zhejiang Provincial Key Laboratory of Plant 
            Virology, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao 
            Road, Hangzhou, Zhejiang 310021, China
REFERENCE   3  (bases 1 to 13415)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13415)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Lett."; date: "2013"; volume: "35"; issue: "7"; pages: "1127-1133"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-OCT-2012) Zhejiang Provincial Key Laboratory of Plant Virology, 
            Zhejiang Academy of Agricultural Sciences, 198 Shiqiao Road, 
            Hangzhou, Zhejiang 310021, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..13415
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        9..2000
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     promoter        2282..2384
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             2385..3062
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     CDS             3385..3687
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     terminator      3869..4510
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     primer_bind     complement(4518..4534)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    4737..4761
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             6062..6688
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7125..8189
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      8258..8452
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    8796..8936
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9122..9710)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9800..10591)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    11016..11040
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(11118..11292)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(11336..12358)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(12426..13102)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    13293..13314
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        13329..13359
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13367..13383
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13391..13407
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattgggct gcagtgcagc gtgacccggt cgtgcccctc tctagagata atgagcattg
       61 catgtctaag ttataaaaaa ttaccacata ttttttttgt cacacttgtt tgaagtgcag
      121 tttatctatc tttatacata tatttaaact ttactctacg aataatataa tctatagtac
      181 tacaataata tcagtgtttt agagaatcat ataaatgaac agttagacat ggtctaaagg
      241 acaattgagt attttgacaa caggactcta cagttttatc tttttagtgt gcatgtgttc
      301 tccttttttt ttgcaaatag cttcacctat ataatacttc atccatttta ttagtacatc
      361 catttagggt ttagggttaa tggtttttat agactaattt ttttagtaca tctattttat
      421 tctattttag cctctaaatt aagaaaacta aaactctatt ttagtttttt tatttaataa
      481 tttagatata aaatagaata aaataaagtg actaaaaatt aaacaaatac cctttaagaa
      541 attaaaaaaa ctaaggaaac atttttcttg tttcgagtag ataatgccag cctgttaaac
      601 gccgtcgacg agtctaacgg acaccaacca gcgaaccagc agcgtcgcgt cgggccaagc
      661 gaagcagacg gcacggcatc tctgtcgctg cctctggacc cctctcgaga gttccgctcc
      721 accgttggac ttgctccgct gtcggcatcc agaaattgcg tggcggagcg gcagacgtga
      781 gccggcacgg caggcggcct cctcctcctc tcacggcacg gcagctacgg gggattcctt
      841 tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa taaatagaca ccccctccac
      901 accctctttc cccaacctcg tgttgttcgg agcgcacaca cacacaacca gatctccccc
      961 aaatccaccc gtcggcacct ccgcttcaag gtacgccgct cgtcctcccc ccccccccct
     1021 ctctaccttc tctagatcgg cgttccggtc catggttagg gcccggtagt tctacttctg
     1081 ttcatgtttg tgttagatcc gtgtttgtgt tagatccgtg ctgctagcgt tcgtacacgg
     1141 atgcgacctg tacgtcagac acgttctgat tgctaacttg ccagtgtttc tctttgggga
     1201 atcctgggat ggctctagcc gttccgcaga cgggatcgat ttcatgattt tttttgtttc
     1261 gttgcatagg gtttggtttg cccttttcct ttatttcaat atatgccgtg cacttgtttg
     1321 tcgggtcatc ttttcatgct tttttttgtc ttggttgtga tgatgtggtc tggttgggcg
     1381 gtcgttctag atcggagtag aattctgttt caaactacct ggtggattta ttaattttgg
     1441 atctgtatgt gtgtgccata catattcata gttacgaatt gaagatgatg gatggaaata
     1501 tcgatctagg ataggtatac atgttgatgc gggttttact gatgcatata cagagatgct
     1561 ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg gtcgttcatt cgttctagat
     1621 cggagtagaa tactgtttca aactacctgg tgtatttatt aattttggaa ctgtatgtgt
     1681 gtgtcataca tcttcatagt tacgagttta agatggatgg aaatatcgat ctaggatagg
     1741 tatacatgtt gatgtgggtt ttactgatgc atatacatga tggcatatgc agcatctatt
     1801 catatgctct aaccttgagt acctatctat tataataaac aagtatgttt tataattatt
     1861 ttgatcttga tatacttgga tgatggcata tgcagcagct atatgtggat ttttttagcc
     1921 ctgccttcat acgctattta tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt
     1981 gtttggtgtt acttctgcag gaattcgata tcaagctatt cgagctccag cagcagccac
     2041 agcaaaattt ggtttgggat aggtaggtgt tatgttaggt ctggtttttt ggctgtagca
     2101 gcagctgcag tgataaggag acggcggtac cgttgacctg cagtaagttg gcagcatcac
     2161 ccgacgcact ttgcgccgaa taaatacctg tgacggaaga tcacttcgca gaataaataa
     2221 atcctggtgt ccctgttgat accgggaagc cctgggccaa cttttggcga aaatgagacg
     2281 ttgatcggca cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg
     2341 tattttttga gtcatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
     2401 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
     2461 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
     2521 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
     2581 tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg
     2641 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctat
     2701 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
     2761 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct
     2821 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
     2881 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc
     2941 cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta
     3001 atgaattaca acagtactgc gatgagtggc aggcggggcg taatctagag gatccggctt
     3061 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     3121 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca
     3181 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     3241 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     3301 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     3361 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     3421 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     3481 gtgatccccc tggccagtgc acgtctgctg tcagataaag tcccccgtga actttacccg
     3541 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     3601 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     3661 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     3721 gcaggtcaac ggtaccgccg tctccttatc actgcacctg ctgctaggct gttctgtgga
     3781 agtttgcaga gtttatatta tgggtttaat cgtccatggc atcagcatca gcagcggtac
     3841 ccggggatcc tctagagtcg acctgcagag ctttcgttcg tatcatcggt ttcgacaacg
     3901 ttcgtcaagt tcaatgcatc agtttcattg cgcacacacc agaatcctac tgagtttgag
     3961 tattatggca ttgggaaaac tgtttttctt gtaccatttg ttgtgcttgt aatttactgt
     4021 gttttttatt cggttttcgc tatcgaactg tgaaatggaa atggatggag aagagttaat
     4081 gaatgatatg gtccttttgt tcattctcaa attaatatta tttgtttttt ctcttatttg
     4141 ttgtgtgttg aatttgaaat tataagagat atgcaaacat tttgttttga gtaaaaatgt
     4201 gtcaaatcgt ggcctctaat gaccgaagtt aatatgagga gtaaaacact tgtagttgta
     4261 ccattatgct tattcactag gcaacaaata tattttcaga cctagaaaag ctgcaaatgt
     4321 tactgaatac aagtatgtcc tcttgtgttt tagacattta tgaactttcc tttatgtaat
     4381 tttccagaat ccttgtcaga ttctaatcat tgctttataa ttatagttat actcatggat
     4441 ttgtagttga gtatgaaaat attttttaat gcattttatg acttgccaat tgattgacaa
     4501 catgcatcaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     4561 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     4621 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgctagagc
     4681 agcttgagct tggatcagat tgtcgtttcc cgccttcagt ttaaactatc agtgtttgac
     4741 aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa tcggatattt
     4801 aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca accacagggt
     4861 tcccctcggg atcaaagtac tttgatccaa cccctccgct gctatagtgc agtcggcttc
     4921 tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta agttacgcga
     4981 caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag
     5041 tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc cgccgctggc
     5101 ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca acgggccgaa
     5161 ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac caggcgcgac
     5221 cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt gacagtgacc
     5281 aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag
     5341 gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc
     5401 ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc
     5461 gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc
     5521 cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc
     5581 accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt
     5641 gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac
     5701 gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca
     5761 tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg
     5821 agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc
     5881 atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg
     5941 ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc
     6001 gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg
     6061 catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac
     6121 ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc
     6181 ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg
     6241 catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt
     6301 gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt
     6361 cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga
     6421 gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc
     6481 gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga
     6541 gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc
     6601 cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct
     6661 ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa
     6721 agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg
     6781 gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat
     6841 cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct
     6901 gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt
     6961 agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg
     7021 ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc
     7081 ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgacggtcgc aaaccatccg
     7141 gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc
     7201 aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg
     7261 ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta
     7321 ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg
     7381 gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc
     7441 gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag
     7501 ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc
     7561 taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc
     7621 gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag
     7681 acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga
     7741 agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct
     7801 acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt
     7861 ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt
     7921 actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag
     7981 gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag
     8041 agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt
     8101 acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc
     8161 tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg
     8221 ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg tacattggga
     8281 acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg
     8341 ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct
     8401 aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg
     8461 gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac
     8521 gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac
     8581 ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc
     8641 cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     8701 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     8761 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
     8821 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     8881 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
     8941 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     9001 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     9061 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     9121 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     9181 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     9241 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     9301 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     9361 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     9421 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     9481 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     9541 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     9601 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     9661 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     9721 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     9781 tcatgcattc taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc
     9841 aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat
     9901 cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc
     9961 tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca
    10021 ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca
    10081 gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca
    10141 gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg
    10201 gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa
    10261 tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac
    10321 tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca
    10381 gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt
    10441 tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata
    10501 ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt
    10561 tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt ttctacagta
    10621 tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc
    10681 attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta
    10741 taacatagta tcgacggagc cgattttgaa accgcggtga tcacaggcag caacgctctg
    10801 tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc
    10861 ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg
    10921 gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg
    10981 ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac
    11041 aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta ctgaattaac
    11101 gccgaattaa ttcgggggat ctggatttta gtactggatt ttggttttag gaattagaaa
    11161 ttttattgat agaagtattt tacaaataca aatacatact aagggtttct tatatgctca
    11221 acacatgagc gaaaccctat aggaacccta attcccttat ctgggaacta ctcacacatt
    11281 attatggaga aactcgagct tgtcgatcga cagatcccgg tcggcatcta ctctatttct
    11341 ttgccctcgg acgagtgctg gggcgtcggt ttccactatc ggcgagtact tctacacagc
    11401 catcggtcca gacggccgcg cttctgcggg cgatttgtgt acgcccgaca gtcccggctc
    11461 cggatcggac gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg aaattgccgt
    11521 caaccaagct ctgatagagt tggtcaagac caatgcggag catatacgcc cggagtcgtg
    11581 gcgatcctgc aagctccgga tgcctccgct cgaagtagcg cgtctgctgc tccatacaag
    11641 ccaaccacgg cctccagaag aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg
    11701 cctcgctcca gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca ttgttggagc
    11761 cgaaatccgc gtgcacgagg tgccggactt cggggcagtc ctcggcccaa agcatcagct
    11821 catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt tgccagtgat
    11881 acacatgggg atcagcaatc gcgcatatga aatcacgcca tgtagtgtat tgaccgattc
    11941 cttgcggtcc gaatgggccg aacccgctcg tctggctaag atcggccgca gcgatcgcat
    12001 ccatagcctc cgcgaccggt tgtagaacag cgggcagttc ggtttcaggc aggtcttgca
    12061 acgtgacacc ctgtgaacgg cgggagatgc aataggtcag gctctcgcta aactccccaa
    12121 tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa acataacgat
    12181 ctttgtagaa accatcggcg cagctattta cccgcaggac atatccacgc cctcctacat
    12241 cgaagctgaa agcacgagat tcttcgccct ccgagagctg catcaggtcg gagacgctgt
    12301 cgaacttttc gatcagaaac ttctcgacag acgtcgcggt gagttcaggc tttttcatat
    12361 ctcattgccc ccccggatct gcgaaagctc gagagagata gatttgtaga gagagactgg
    12421 tgatttcagc gtgtcctctc caaatgaaat gaacttcctt atatagagga agggtcttgc
    12481 gaaggatagt gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact
    12541 tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt
    12601 ccatctttgg gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa
    12661 tgatggcatt tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata
    12721 gctgggcaat ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata
    12781 gccctttggt cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc
    12841 tccaccatgt tcacatcaat ccacttgctt tgaagacgtg gttggaacgt cttctttttc
    12901 cacgatgctc ctcgtgggtg ggggtccatc tttgggacca ctgtcggcag aggcatcttg
    12961 aacgatagcc tttcctttat cgcaatgatg gcatttgtag gtgccacctt ccttttctac
    13021 tgtccttttg atgaagtgac agatagctgg gcaatggaat ccgaggaggt ttcccgatat
    13081 taccctttgt tgaaaagtct caatagccct ttggtcttct gagactgtat ctttgatatt
    13141 cttggagtag acgagagtgt cgtgctccac catgttggca agctgctcta gccaatacgc
    13201 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
    13261 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
    13321 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
    13381 caatttcaca caggaaacag ctatgacatg attac
//
