LOCUS       40924_44664       12347 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pUA-At, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12347)
  AUTHORS   Zhou J, Yu F, Chen B, Wang X, Yang Y, Cheng Y, Yan C, Chen J.
  TITLE     Universal vectors for constructing artificial microRNAs in plants
  JOURNAL   Biotechnol. Lett. 35 (7), 1127-1133 (2013)
  PUBMED    23568377
REFERENCE   2  (bases 1 to 12347)
  AUTHORS   Zhou J, Wang XM, Yan CQ, Chen JP.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-OCT-2012) Zhejiang Provincial Key Laboratory of Plant 
            Virology, Zhejiang Academy of Agricultural Sciences, 198 Shiqiao 
            Road, Hangzhou, Zhejiang 310021, China
REFERENCE   3  (bases 1 to 12347)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12347)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol.
            Lett."; date: "2013"; volume: "35"; issue: "7"; pages: "1127-1133"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-OCT-2012) Zhejiang Provincial Key Laboratory of Plant Virology, 
            Zhejiang Academy of Agricultural Sciences, 198 Shiqiao Road, 
            Hangzhou, Zhejiang 310021, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..12347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        501..846
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    868..968
                     /label=amiRNA backbone
                     /note="amiRNA backbone"
     promoter        1145..1247
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             1248..1925
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     CDS             2248..2550
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     misc_feature    2620..2774
                     /label=amiRNA backbone
                     /note="amiRNA backbone"
     terminator      2801..3442
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     primer_bind     complement(3450..3466)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3669..3693
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             4993..5619
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6056..7120
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      7189..7383
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    7727..7867
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8053..8641)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8731..9522)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    9947..9971
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(10049..10223)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(10266..11288)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(11356..12033)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    12224..12245
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        12260..12290
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12298..12314
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12322..12338
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcggtc cccagattag ccttttcaat ttcagaaaga atgctaaccc acagatggtt
       61 agagaggctt acgcagcagg tctcatcaag acgatctacc cgagcaataa tctccaggaa
      121 atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa gattcaggac taactgcatc
      181 aagaacacag agaaagatat atttctcaag atcagaagta ctattccagt atggacgatt
      241 caaggcttgc ttcacaaacc aaggcaagta atagagattg gagtctctaa aaaggtagtt
      301 cccactgaat caaaggccat ggagtcaaag attcaaatag aggacctaac agaactcgcc
      361 gtaaagactg gcgaacagtt catacagagt ctcttacgac tcaatgacaa gaagaaaatc
      421 ttcgtcaaca tggtggagca cgacacactt gtctactcca aaaatatcaa agatacagtc
      481 tcagaagacc aaagggcaat tgagactttt caacaaaggg taatatccgg aaacctcctc
      541 ggattccatt gcccagctat ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc
      601 tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc ctctgccgac
      661 agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca
      721 accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca
      781 caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag
      841 agaacacggg ggacgagctc ggtacccatg aattcctgca gccccaaaca cacgctcgga
      901 cgcatattac acatgttcat acacttaata ctcgctgttt tgaattgatg ttttaggaat
      961 atatatgtag cagtgataag gagacggcgg taccgttgac ctgcagtaag ttggcagcat
     1021 cacccgacgc actttgcgcc gaataaatac ctgtgacgga agatcacttc gcagaataaa
     1081 taaatcctgg tgtccctgtt gataccggga agccctgggc caacttttgg cgaaaatgag
     1141 acgttgatcg gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg
     1201 gcgtattttt tgagtcatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa
     1261 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
     1321 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
     1381 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
     1441 gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat
     1501 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc
     1561 tatggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
     1621 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg
     1681 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
     1741 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga
     1801 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc
     1861 ttaatgaatt acaacagtac tgcgatgagt ggcaggcggg gcgtaatcta gaggatccgg
     1921 cttactaaaa gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat
     1981 atatactgat atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt
     2041 acagtgacag ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct
     2101 ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga
     2161 aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt
     2221 ttgctgacga gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag
     2281 agccgttatc gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg
     2341 atggtgatcc ccctggccag tgcacgtctg ctgtcagata aagtcccccg tgaactttac
     2401 ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg
     2461 ccggtctccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa
     2521 aacgccatta acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag
     2581 tctgcaggtc aacggtaccg ccgtctcctt atcactgctc tcttttgtat tccaattttc
     2641 ttgattaatc tttcctgcac aaaaacatgc ttgatccact aagtgacata tatgctgcct
     2701 tcgtatatat agttctggta aaattaacat tttgggttta tctttattta aggcatcgcc
     2761 atggggggat ccctggggat cctctagagt cgacctgcag agctttcgtt cgtatcatcg
     2821 gtttcgacaa cgttcgtcaa gttcaatgca tcagtttcat tgcgcacaca ccagaatcct
     2881 actgagtttg agtattatgg cattgggaaa actgtttttc ttgtaccatt tgttgtgctt
     2941 gtaatttact gtgtttttta ttcggttttc gctatcgaac tgtgaaatgg aaatggatgg
     3001 agaagagtta atgaatgata tggtcctttt gttcattctc aaattaatat tatttgtttt
     3061 ttctcttatt tgttgtgtgt tgaatttgaa attataagag atatgcaaac attttgtttt
     3121 gagtaaaaat gtgtcaaatc gtggcctcta atgaccgaag ttaatatgag gagtaaaaca
     3181 cttgtagttg taccattatg cttattcact aggcaacaaa tatattttca gacctagaaa
     3241 agctgcaaat gttactgaat acaagtatgt cctcttgtgt tttagacatt tatgaacttt
     3301 cctttatgta attttccaga atccttgtca gattctaatc attgctttat aattatagtt
     3361 atactcatgg atttgtagtt gagtatgaaa atatttttta atgcatttta tgacttgcca
     3421 attgattgac aacatgcatc aagcttggca ctggccgtcg ttttacaacg tcgtgactgg
     3481 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     3541 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     3601 gaatgctaga gcagcttgag cttggatcag attgtcgttt cccgccttca gtttaaacta
     3661 tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat
     3721 aacggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc
     3781 aaccacaggg ttcccctcgg gatcaaagta ctttgatcca acccctccgc tgctatagtg
     3841 cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct
     3901 aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta
     3961 gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg
     4021 ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc
     4081 aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca
     4141 ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct ggcgacgttg
     4201 tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg gacattgccg
     4261 agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg gccgacacca
     4321 ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt
     4381 ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt
     4441 ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag ctgatcgacc
     4501 aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt
     4561 accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct
     4621 tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat gaacgccaag
     4681 aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca ttaccgaaga
     4741 gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac
     4801 cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg cctggccggc
     4861 cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta
     4921 aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg
     4981 caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg
     5041 accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc
     5101 gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta
     5161 accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc
     5221 gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag
     5281 gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc
     5341 gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc
     5401 tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg
     5461 gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc
     5521 actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag
     5581 gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga
     5641 aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg
     5701 ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc
     5761 cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg
     5821 agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt
     5881 agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac
     5941 gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt
     6001 gtctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgacggtcg
     6061 caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt
     6121 gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc
     6181 gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc
     6241 gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct
     6301 ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc
     6361 gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga
     6421 ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc
     6481 ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg
     6541 ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg
     6601 aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat
     6661 gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt
     6721 gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga
     6781 gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt
     6841 tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg
     6901 ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg
     6961 cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga
     7021 cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg
     7081 ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct
     7141 agggcaaatt gccctagcag gggaaaaagg tcgaaaaggt ctctttcctg tggatagcac
     7201 gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa
     7261 gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga
     7321 tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc
     7381 ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct
     7441 gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat
     7501 ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga
     7561 ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
     7621 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     7681 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
     7741 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
     7801 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     7861 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7921 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7981 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     8041 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     8101 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     8161 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     8221 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     8281 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8341 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8401 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8461 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     8521 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8581 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8641 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8701 agggattttg gtcatgcatt ctaggtacta aaacaattca tccagtaaaa tataatattt
     8761 tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc
     8821 ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc
     8881 cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt
     8941 gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa
     9001 aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc
     9061 cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct
     9121 attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata
     9181 cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc
     9241 atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt
     9301 tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata
     9361 ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac
     9421 cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc
     9481 gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct
     9541 tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca
     9601 ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca
     9661 aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg atcacaggca
     9721 gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca
     9781 aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc
     9841 gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg
     9901 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt
     9961 gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt
    10021 actgaattaa cgccgaatta attcggggga tctggatttt agtactggat tttggtttta
    10081 ggaattagaa attttattga tagaagtatt ttacaaatac aaatacatac taagggtttc
    10141 ttatatgctc aacacatgag cgaaacccta taggaaccct aattccctta tctgggaact
    10201 actcacacat tattatggag aaactcgagc ttgtcgatcg acagatccgg tcggcatcta
    10261 ctctatttct ttgccctcgg acgagtgctg gggcgtcggt ttccactatc ggcgagtact
    10321 tctacacagc catcggtcca gacggccgcg cttctgcggg cgatttgtgt acgcccgaca
    10381 gtcccggctc cggatcggac gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg
    10441 aaattgccgt caaccaagct ctgatagagt tggtcaagac caatgcggag catatacgcc
    10501 cggagtcgtg gcgatcctgc aagctccgga tgcctccgct cgaagtagcg cgtctgctgc
    10561 tccatacaag ccaaccacgg cctccagaag aagatgttgg cgacctcgta ttgggaatcc
    10621 ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca
    10681 ttgttggagc cgaaatccgc gtgcacgagg tgccggactt cggggcagtc ctcggcccaa
    10741 agcatcagct catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt
    10801 tgccagtgat acacatgggg atcagcaatc gcgcatatga aatcacgcca tgtagtgtat
    10861 tgaccgattc cttgcggtcc gaatgggccg aacccgctcg tctggctaag atcggccgca
    10921 gcgatcgcat ccatagcctc cgcgaccggt tgtagaacag cgggcagttc ggtttcaggc
    10981 aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc aataggtcag gctctcgcta
    11041 aactccccaa tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa
    11101 acataacgat ctttgtagaa accatcggcg cagctattta cccgcaggac atatccacgc
    11161 cctcctacat cgaagctgaa agcacgagat tcttcgccct ccgagagctg catcaggtcg
    11221 gagacgctgt cgaacttttc gatcagaaac ttctcgacag acgtcgcggt gagttcaggc
    11281 tttttcatat ctcattgccc ccccggatct gcgaaagctc gagagagata gatttgtaga
    11341 gagagactgg tgatttcagc gtgtcctctc caaatgaaat gaacttcctt atatagagga
    11401 aggtcttgcg aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatatcacat
    11461 caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg
    11521 ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct
    11581 ttatcgcaat gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag
    11641 tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa
    11701 gtctcaatag ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga
    11761 gtgtcgtgct ccaccatgtt atcacatcaa tccacttgct ttgaagacgt ggttggaacg
    11821 tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca
    11881 gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct
    11941 tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg
    12001 tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta
    12061 tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttggc aagctgctct
    12121 agccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
    12181 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
    12241 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
    12301 tgagcggata acaatttcac acaggaaaca gctatgacca tgattac
//
