LOCUS       62056_21755       10347 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10347)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        80..459
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        460..663
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             746..4849
                     /codon_start=1
                     /label=Cas9
                     /note="Cas9 (Csn1) endonuclease from the Streptococcus
                     pyogenes Type II CRISPR/Cas system"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4886..4912
                     /codon_start=1
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
                     /translation="YPYDVPDYA"
     promoter        4934..5263
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             5327..6034
                     /codon_start=1
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    6066..6652
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             6666..7262
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    7499..7620
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             7775..8632
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8806..9394
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    9682..9703
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9718..9748
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     promoter        9816..9834
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_RNA        10264..10339
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 agacacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
       61 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
      121 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
      181 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
      241 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
      301 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
      361 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
      421 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
      481 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
      541 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
      601 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
      661 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
      721 tatagggaga cccaagcttg ccaccatgga caagaagtac agcatcggcc tggacatcgg
      781 taccaacagc gtgggctggg ccgtgatcac cgacgagtac aaggtgccca gcaagaagtt
      841 caaggtgctg ggcaacaccg accgccacag catcaagaag aacctgatcg gcgccctgct
      901 gttcgacagc ggcgagaccg ccgaggccac ccgcctgaag cgcaccgccc gccgccgcta
      961 cacccgccgc aagaaccgca tctgctacct gcaggagatc ttcagcaacg agatggccaa
     1021 ggtggacgac agcttcttcc accgcctgga ggagagcttc ctggtggagg aggacaagaa
     1081 gcacgagcgc caccccatct tcggcaacat cgtggacgag gtggcctacc acgagaagta
     1141 ccccaccatc taccacctgc gcaagaagct ggtggacagc accgacaagg ccgacctgcg
     1201 cctgatctac ctggccctgg cccacatgat caagttccgc ggccacttcc tgatcgaggg
     1261 cgacctgaac cccgacaaca gcgacgtgga caagctgttc atccagctgg tgcagaccta
     1321 caaccagctg ttcgaggaga accccatcaa cgccagcggc gtggacgcca aggccatcct
     1381 gagcgcccgc ctgagcaaga gccgccgcct ggagaacctg atcgcccagc tgcccggcga
     1441 gaagaagaac ggcctgttcg gcaacctgat cgccctgagc ctgggcctga cccccaactt
     1501 caagagcaac ttcgacctgg ccgaggacgc caagctgcag ctgagcaagg acacctacga
     1561 cgacgacctg gacaacctgc tggcccagat cggcgaccag tacgccgacc tgttcctggc
     1621 cgccaagaac ctgagcgacg ccatcctgct gagcgacatc ctgcgcgtga acaccgagat
     1681 caccaaggcc cccctgagcg ccagcatgat caagcgctac gacgagcacc accaggacct
     1741 gaccctgctg aaggccctgg tgcgccagca gctgcccgag aagtacaagg agatcttctt
     1801 cgaccagagc aagaacggct acgccggcta catcgacggc ggcgccagcc aggaggagtt
     1861 ctacaagttc atcaagccca tcctggagaa gatggacggc accgaggagc tgctggtgaa
     1921 gctgaaccgc gaggacctgc tgcgcaagca gcgcaccttc gacaacggca gcatccccca
     1981 ccagatccac ctgggcgagc tgcacgccat cctgcgccgc caggaggact tctacccctt
     2041 cctgaaggac aaccgcgaga agatcgagaa gatcctgacc ttccgcatcc cctactacgt
     2101 gggccccctg gcccgcggca acagccgctt cgcctggatg acccgcaaga gcgaggagac
     2161 catcaccccc tggaacttcg aggaggtggt ggacaagggc gccagcgccc agagcttcat
     2221 cgagcgcatg accaacttcg acaagaacct gcccaacgag aaggtgctgc ccaagcacag
     2281 cctgctgtac gagtacttca ccgtgtacaa cgagctgacc aaggtgaagt acgtgaccga
     2341 gggcatgcgc aagcccgcct tcctgagcgg cgagcagaag aaggccatcg tggacctgct
     2401 gttcaagacc aaccgcaagg tgaccgtgaa gcagctgaag gaggactact tcaagaagat
     2461 cgagtgcttc gacagcgtgg agatcagcgg cgtggaggac cgcttcaacg ccagcctggg
     2521 cacctaccac gacctgctga agatcatcaa ggacaaggac ttcctggaca acgaggagaa
     2581 cgaggacatc ctggaggaca tcgtgctgac cctgaccctg ttcgaggacc gcgagatgat
     2641 cgaggagcgc ctgaagacct acgcccacct gttcgacgac aaggtgatga agcagctgaa
     2701 gcgccgccgc tacaccggct ggggccgcct gagccgcaag cttatcaacg gcatccgcga
     2761 caagcagagc ggcaagacca tcctggactt cctgaagagc gacggcttcg ccaaccgcaa
     2821 cttcatgcag ctgatccacg acgacagcct gaccttcaag gaggacatcc agaaggccca
     2881 ggtgagcggc cagggcgaca gcctgcacga gcacatcgcc aacctggccg gcagccccgc
     2941 catcaagaag ggcatcctgc agaccgtgaa ggtggtggac gagctggtga aggtgatggg
     3001 ccgccacaag cccgagaaca tcgtgatcga gatggcccgc gagaaccaga ccacccagaa
     3061 gggccagaag aacagccgcg agcgcatgaa gcgcatcgag gagggcatca aggagctggg
     3121 cagccagatc ctgaaggagc accccgtgga gaacacccag ctgcagaacg agaagctgta
     3181 cctgtactac ctgcagaacg gccgcgacat gtacgtggac caggagctgg acatcaaccg
     3241 cctgagcgac tacgacgtgg accacatcgt gccccagagc ttcctgaagg acgacagcat
     3301 cgacaacaag gtgctgaccc gcagcgacaa gaaccgcggc aagagcgaca acgtgcccag
     3361 cgaggaggtg gtgaagaaga tgaagaacta ctggcgccag ctgctgaacg ccaagctgat
     3421 cacccagcgc aagttcgaca acctgaccaa ggccgagcgc ggcggcctga gcgagctgga
     3481 caaggccggc ttcatcaagc gccagctggt ggagacccgc cagatcacca agcacgtggc
     3541 ccagatcctg gacagccgca tgaacaccaa gtacgacgag aacgacaagc tgatccgcga
     3601 ggtgaaggtg atcaccctga agagcaagct ggtgagcgac ttccgcaagg acttccagtt
     3661 ctacaaggtg cgcgagatca acaactacca ccacgcccac gacgcctacc tgaacgccgt
     3721 ggtgggcacc gccctgatca agaagtaccc caagctggag agcgagttcg tgtacggcga
     3781 ctacaaggtg tacgacgtgc gcaagatgat cgccaagagc gagcaggaga tcggcaaggc
     3841 caccgccaag tacttcttct acagcaacat catgaacttc ttcaagaccg agatcaccct
     3901 ggccaacggc gagatccgca agcgccccct gatcgagacc aacggcgaga ccggcgagat
     3961 cgtgtgggac aagggccgcg acttcgccac cgtgcgcaag gtgctgagca tgccccaggt
     4021 gaacatcgtg aagaagaccg aggtgcagac cggcggcttc agcaaggaga gcatcctgcc
     4081 caagcgcaac agcgacaagc tgatcgcccg caagaaggac tgggacccca agaagtacgg
     4141 cggcttcgac agccccaccg tggcctacag cgtgctggtg gtggccaagg tggagaaggg
     4201 caagagcaag aagctgaaga gcgtgaagga gctgctgggc atcaccatca tggagcgcag
     4261 cagcttcgag aagaacccca tcgacttcct ggaggccaag ggctacaagg aggtgaagaa
     4321 ggacctgatc atcaagctgc ccaagtacag cctgttcgag ctggagaacg gccgcaagcg
     4381 catgctggcc agcgccggcg agctgcagaa gggcaacgag ctggccctgc ccagcaagta
     4441 cgtgaacttc ctgtacctgg ccagccacta cgagaagctg aagggcagcc ccgaggacaa
     4501 cgagcagaag cagctgttcg tggagcagca caagcactac ctggacgaga tcatcgagca
     4561 gatcagcgag ttcagcaagc gcgtgatcct ggccgacgcc aacctggaca aggtgctgag
     4621 cgcctacaac aagcaccgcg acaagcccat ccgcgagcag gccgagaaca tcatccacct
     4681 gttcaccctg accaacctgg gcgcccccgc cgccttcaag tacttcgaca ccaccatcga
     4741 ccgcaagcgc tacaccagca ccaaggaggt gctggacgcc accctgatcc accagagcat
     4801 caccggtctg tacgagaccc gcatcgacct gagccagctg ggcggcgacg gcggctccgg
     4861 acctccaaag aaaaagagaa aagtataccc ctacgacgtg cccgactacg cctaataact
     4921 cgagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     4981 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     5041 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     5101 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     5161 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     5221 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     5281 tatatccatt ttcggatctg atcggcgcgc cataccggtc gccaccatgg tgagcaaggg
     5341 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     5401 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     5461 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     5521 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     5581 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     5641 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     5701 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     5761 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     5821 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     5881 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     5941 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     6001 ccactccacc ggcggcatgg acgagctgta caagtaggcg gccgcatcta gggcggccaa
     6061 ttccgcccct ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc
     6121 cggtgtgcgt ttgtctatat gtgattttcc accatattgc cgtcttttgg caatgtgagg
     6181 gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc
     6241 aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga
     6301 agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg
     6361 tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag
     6421 tgccacgttg tgggttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc
     6481 aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct
     6541 cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac
     6601 cacggggacg tggttttcct ttgaaaaaca cgatgataag cttgccacaa cccacaagac
     6661 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg
     6721 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc
     6781 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     6841 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     6901 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     6961 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     7021 aggagcccgc gtggttcctg gccaccgtcg gtgtctcgcc cgaccaccag ggcaagggtc
     7081 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     7141 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     7201 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     7261 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct
     7321 ccgaccgaag ccacccgggg cggccccgcc gaccccgcac ccgcccccga ggcccaccga
     7381 ctctagataa ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     7441 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     7501 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     7561 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     7621 acgcgtcgag tgcattctag tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     7681 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     7741 taaaggtaag cctgaatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     7801 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     7861 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     7921 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     7981 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     8041 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     8101 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     8161 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     8221 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     8281 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     8341 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     8401 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     8461 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     8521 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     8581 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     8641 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     8701 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     8761 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     8821 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggttttttt
     8881 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     8941 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     9001 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     9061 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     9121 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     9181 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     9241 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     9301 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     9361 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     9421 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     9481 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     9541 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     9601 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     9661 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     9721 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     9781 aggaaacagc tatgacatga ttacgaattg caacgattta ggtgacacta tagaagagaa
     9841 ggaattaata cgactcacta tagggagaga gagagaatta ccctcactaa agggaggaga
     9901 agcatgaatt ccccagtgga aagacgcgca ggcaaaacgc accacgtgac ggagcgtgac
     9961 cgcgcgccga gcgcgcgcca aggtcgggca ggaagagggc ctatttccca tgattccttc
    10021 atatttgcat atacgataca aggctgttag agagataatt agaattaatt tgactgtaaa
    10081 cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc
    10141 agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt
    10201 cgatttcttg ggtttatata tcttgtggaa aggacgagga tccggagacg aagcttcgtc
    10261 tctgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa
    10321 agtggcaccg agtcggtgct tttttct
//
