LOCUS       62056_21735        5753 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..242
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     primer_bind     complement(336..352)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(389..407)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    complement(414..535)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(674..1390)
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     promoter        complement(1435..1638)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(1639..1942)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(2117..2705)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2879..3736)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3737..3841)
                     /label=AmpR promoter
     rep_origin      complement(3867..4322)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     4464..4480
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4490..4508
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     4541..4557
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(4623..5219)
                     /codon_start=1
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     promoter        complement(5244..5743)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt tttaaaatta tgttttaaaa tggactatca
      181 tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg
      241 acgaaacacc gacaagatga agagcaccaa ttcaagagat tggtgctctt catcttgttt
      301 ttttaagctt gatatcgaat tcctgcagcc cgggggatcc actagttcta gagcggccgc
      361 caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg cgttaagata
      421 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
      481 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
      541 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
      601 caagtaaaac ctctacaaat gtggtatggc tgattatgat cagttatcta gatccggtgg
      661 atctgagtcc ggacttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga
      721 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggg
      781 tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct
      841 ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca
      901 ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt
      961 actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga
     1021 tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt
     1081 cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga
     1141 agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg taggtcaggg
     1201 tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg
     1261 tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt
     1321 ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct
     1381 tgctcaccat ggtggcgacc ggtagcgcta gcggatctga cggttcacta aaccagctct
     1441 gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt
     1501 gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc
     1561 aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta
     1621 ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt
     1681 aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt
     1741 gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt
     1801 ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg
     1861 aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc
     1921 atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt
     1981 aattgattac tattaataac taatgcatgg cggtaatacg gttatccaca gaatcagggg
     2041 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     2101 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     2161 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     2221 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     2281 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     2341 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     2401 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     2461 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     2521 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     2581 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     2641 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     2701 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     2761 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     2821 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     2881 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     2941 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     3001 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     3061 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     3121 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     3181 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     3241 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     3301 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     3361 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     3421 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     3481 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     3541 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     3601 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     3661 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     3721 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     3781 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     3841 gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc
     3901 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc
     3961 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag
     4021 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga
     4081 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc
     4141 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa
     4201 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt
     4261 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
     4321 gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     4381 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     4441 agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc
     4501 actatagggc gaattggagc tccaccgcgg tggcggccgc tctagaacta gtggatcccc
     4561 cgggctgcat ggggtcgtgc gctcctttcg gtcgggcgct gcgggtcgtg gggcgggcgt
     4621 caggcaccgg gcttgcgggt catgcaccag gtgcgcggtc cttcgggcac ctcgacgtcg
     4681 gcggtgacgg tgaagccgag ccgctcgtag aaggggaggt tgcggggcgc ggaggtctcc
     4741 aggaaggcgg gcaccccggc gcgctcggcc gcctccactc cggggagcac gacggcgctg
     4801 cccagaccct tgccctggtg gtcgggcgag acgccgacgg tggccaggaa ccacgcgggc
     4861 tccttgggcc ggtgcggcgc caggaggcct tccatctgtt gctgcgcggc cagccgggaa
     4921 ccgctcaact cggccatgcg cgggccgatc tcggcgaaca ccgcccccgc ttcgacgctc
     4981 tccggcgtgg tccagaccgc caccgcggcg ccgtcgtccg cgacccacac cttgccgatg
     5041 tcgagcccga cgcgcgtgag gaagagttct tgcagctcgg tgacccgctc gatgtggcgg
     5101 tccggatcga cggtgtggcg cgtggcgggg tagtcggcga acgcggcggc gagggtgcgt
     5161 acggccctgg ggacgtcgtc gcgggtggcg aggcgcaccg tgggcttgta ctcggtcatg
     5221 gtaagctagc ttgggctgca ggtcgaaagg cccggagatg aggaagagga gaacagcgcg
     5281 gcagacgtgc gcttttgaag cgtgcagaat gccgggcctc cggaggacct tcgggcgccc
     5341 gccccgcccc tgagcccgcc cctgagcccg cccccggacc caccccttcc cagcctctga
     5401 gcccagaaag cgaaggagca aagctgctat tggccgctgc cccaaaggcc tacccgcttc
     5461 cattgctcag cggtgctgtc catctgcacg agactagtga gacgtgctac ttccatttgt
     5521 cacgtcctgc acgacgcgag ctgcggggcg ggggggaact tcctgactag gggaggagta
     5581 gaaggtggcg cgaaggggcc accaaagaac ggagccggtt ggcgcctacc ggtggatgtg
     5641 gaatgtgtgc gaggccagag gccacttgtg tagcgccaag tgcccagcgg ggctgctaaa
     5701 gcgcatgctc cagactgcct tgggaaaagc gcctccccta cccggtagaa ttc
//
