LOCUS       V002483                 7910 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V002483
VERSION     V002483
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7910)
  AUTHORS   van Tuyn J, Pijnappels DA, de Vries AA, de Vries I, van der
            Velde-van Dijke I, Knaan-Shanzer S, van der Laarse A, Schalij MJ,
            Atsma DE.
  TITLE     Post MI scar fibroblasts can be differentiated into
            cardiomyocyte-like cells using myocardin gene transfer
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7910)
  AUTHORS   van Tuyn J.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2007) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300
            RC, Netherlands
REFERENCE   3  (bases 1 to 7910)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7910)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (05-JAN-2007) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300 RC,
            Netherlands"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7910
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     enhancer        68..447
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        450..725
                     /label="chicken beta-actin promoter"
     intron          726..1734
                     /label="chimeric intron"
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     CDS             1852..4665
                     /gene="MYOCD"
                     /label="Myocardin"
                     /note="Myocardin from Homo sapiens. Accession#: Q8IZQ8"
     polyA_signal    4879..4934
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(5311..5327)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5801..5905
                     /label="AmpR promoter"
     CDS             5906..6763
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6937..7525
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    7813..7834
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7849..7879
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7887..7903
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 caggaaacag ctatgaccat gattacgcca agcttgttta aacatttaaa tctcgagcca
       61 tggattcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
      121 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      181 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      241 atagggactt tccattgacg tcaatgggtg gactatttac ggtaaactgc ccacttggca
      301 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      361 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      421 tacgtattag tcatcgctat taccatgggt cgaggtgagc cccacgttct gcttcactct
      481 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
      541 tgcagcgatg ggggcggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg
      601 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
      661 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
      721 gggcgggagt cgctgcgttg ccttcgcccc gtgccccgct ccgcgccgcc tcgcgccgcc
      781 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      841 ctccgggctg taattagcgc ttggtttaat gacggctcgt ttcttttctg tggctgcgtg
      901 aaagccttaa agggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg
      961 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc
     1021 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga gcgcggccgg
     1081 gggcggtgcc ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     1141 tgcgtggggg ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc cccccctgca
     1201 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtgcggggcg
     1261 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1321 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccg gagcgccggc
     1381 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
     1441 cagggacttc ctttgtccca aatctggcgg agccgaaatc tgggaggcgc cgccgcaccc
     1501 cctctagcgg gcgcgggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     1561 ccttcgtgcg tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgccgcag
     1621 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1681 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1741 ggcaacgtgc tggttgttgt gctgtctcat cattttggca aagaattatc gcatgcctgc
     1801 agagctctag agtcgcctgg ccaagggacc agcggcttgc tgagactcaa catgacactc
     1861 ctggggtctg agcattcctt gctgattagg agcaagttca gatcagtttt acagttaaga
     1921 cttcaacaaa gaaggaccca ggaacaactg gctaaccaag gcataatacc accactgaaa
     1981 cgtccagctg aattccatga gcaaagaaaa catttggata gtgacaaggc taaaaattcc
     2041 ctgaagcgca aagccagaaa caggtgcaac agtgccgact tggttaatat gcacatactc
     2101 caagcttcca ctgccgagag gtccattcca actgctcaga tgaagctgaa aagagcccga
     2161 ctcgccgatg atctcaatga aaaaattgct ctacgaccag ggccactgga gctggtggaa
     2221 aaaaacattc ttcctgtgga ttctgctgtg aaagaggcca taaaaggtaa ccaggtgagt
     2281 ttctccaaat ccacggatgc ttttgccttt gaagaggaca gcagcagcga tgggctttct
     2341 ccggatcaga ctcgaagtga agacccccaa aactcagcgg gatccccgcc agacgctaaa
     2401 gcctcagata ccccttcgac aggttctctg gggacaaacc aggatcttgc ttctggctca
     2461 gaaaatgaca gaaatgactc agcctcacag cccagccacc agtcagatgc ggggaagcag
     2521 gggcttggcc cccccagcac ccccatagcc gtgcatgctg ctgtaaagtc caaatccttg
     2581 ggtgacagta agaaccgcca caaaaagccc aaggacccca agccaaaggt gaagaagctt
     2641 aaatatcacc agtacattcc cccagaccag aaggcagaga agtcccctcc acctatggac
     2701 tcagcctacg ctcggctgct ccagcaacag cagctgttcc tgcagctcca aatcctcagc
     2761 cagcagcagc agcagcagca acaccgattc agctacctag ggatgcacca agctcagctt
     2821 aaggaaccaa atgaacagat ggtcagaaat ccaaactctt cttcaacgcc actgagcaat
     2881 acccccttgt ctcctgtcaa aaacagtttt tctggacaaa ctggtgtctc ttctttcaaa
     2941 ccaggcccac tcccacctaa cctggatgat ctgaaggtct ctgaattaag acaacagctt
     3001 cgaattcggg gcttgcctgt gtcaggcacc aaaacggctc tcatggaccg gcttcgaccc
     3061 ttccaggact gctctggcaa cccagtgccg aactttgggg atataacgac tgtcactttt
     3121 cctgtcacac ccaacacgct gcccaattac cagtcttcct cttctaccag tgccctgtcc
     3181 aacggcttct accactttgg cagcaccagc tccagccccc cgatctcccc agcctcctct
     3241 gacctgtcag tcgctgggtc cctgccggac accttcaatg atgcctcccc ctccttcggc
     3301 ctgcacccgt ccccagtcca cgtgtgcacg gaggaaagtc tcatgagcag cctgaatggg
     3361 ggctctgttc cttctgagct ggatgggctg gactccgaga aggacaagat gctggtggag
     3421 aagcagaagg tgatcaatga actcacctgg aaactccagc aagagcagag gcaggtggag
     3481 gagctgagga tgcagcttca gaagcagaaa aggaataact gttcagagaa gaagccgctg
     3541 cctttcctgg ctgcctccat caagcaggaa gaggctgtct ccagctgtcc ttttgcatcc
     3601 caagtacctg tgaaaagaca aagcagcagc tcagagtgtc acccaccggc ttgtgaagct
     3661 gctcaactcc agcctcttgg aaatgctcat tgtgtggagt cctcagatca aaccaatgta
     3721 ctttcttcca catttctcag cccccagtgt tcccctcagc attcaccgct gggggctgtg
     3781 aaaagcccac agcacatcag tttgccccca tcacccaaca accctcactt tctgccctca
     3841 tcctccgggg cccagggaga agggcacagg gtctcctcgc ccatcagcag ccaggtgtgc
     3901 actgcacaga tggctggttt acactcttct gataaggtgg ggccaaagtt ttcaattcca
     3961 tccccaactt tttctaagtc aagttcagca atttcagagg taacacagcc tccatcctat
     4021 gaagatgccg taaagcagca aatgacccgg agtcagcaga tggatgaact cctggacgtg
     4081 cttattgaaa gcggagaaat gccagcagac gctagagagg atcactcatg tcttcaaaaa
     4141 gtcccaaaga tacccagatc ttcccgaagt ccaactgctg tcctcaccaa gccctcggct
     4201 tcctttgaac aagcctcttc aggcagccag atcccctttg atccctatgc caccgacagt
     4261 gatgagcatc ttgaagtctt attaaattcc cagagccccc taggaaagat gagtgatgtc
     4321 acccttctaa aaattgggag cgaagagcct cactttgatg ggataatgga tggattctct
     4381 gggaaggctg cagaagacct cttcaatgca catgagatct tgccaggccc cctctctcca
     4441 atgcagacac agttttcacc ctcttctgtg gacagcaatg ggctgcagtt aagcttcact
     4501 gaatctccct gggaaaccat ggagtggctg gacctcactc cgccaaattc cacaccaggc
     4561 tttagcgccc tcaccaccag cagccccagc atcttcaaca tcgatttcct ggatgtcact
     4621 gatctcaatt tgaattcttc catggacctt cacttgcagc agtggtagaa tgcccaatgc
     4681 accagtgcta tggaagacca atggagaaat cccgggatcc acgcgtggat caattcactc
     4741 ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca
     4801 aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg
     4861 agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt
     4921 ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg
     4981 agtatttggt ttagagtttg gcaacatatg ccatatgctg gctgccatga acaaaggtgg
     5041 ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga
     5101 aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta
     5161 acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta
     5221 ctcccagtca tagctgtccc tcttctctta tgaagatccc tcgacctgca gcccaagctg
     5281 atcccgggat ttaaatgttt aaacgaattc actggccgtc gttttacaac gtcgtgactg
     5341 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     5401 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     5461 cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     5521 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
     5581 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     5641 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     5701 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
     5761 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     5821 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     5881 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     5941 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     6001 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     6061 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     6121 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     6181 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     6241 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     6301 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     6361 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     6421 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     6481 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     6541 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     6601 atctggagcc cgtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     6661 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     6721 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     6781 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     6841 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     6901 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     6961 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     7021 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     7081 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     7141 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     7201 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     7261 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     7321 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     7381 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     7441 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     7501 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     7561 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     7621 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     7681 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     7741 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     7801 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     7861 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
//
