LOCUS       40924_44634        5947 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pU.CAG.Hand1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   van Tuyn J, Pijnappels DA, de Vries AA, de Vries I, van der 
            Velde-van Dijke I, Knaan-Shanzer S, van der Laarse A, Schalij MJ, 
            Atsma DE.
  TITLE     Post MI scar fibroblasts can be differentiated into 
            cardiomyocyte-like cells using myocardin gene transfer
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5947)
  AUTHORS   van Tuyn J.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JAN-2007) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300 
            RC, Netherlands
REFERENCE   3  (bases 1 to 5947)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5947)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-JAN-2007) MCB, LUMC, Einthovenweg 20, Leiden, ZH 2300 RC, 
            Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5947
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        68..447
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        450..725
                     /label=chicken beta-actin promoter
     intron          726..1734
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
     CDS             1919..2563
                     /gene="HAND1"
                     /label=HAND1
                     /note="Heart- and neural crest derivatives-expressed
                     protein 1 from Homo sapiens. Accession#: O96004"
     polyA_signal    2916..2971
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3348..3364)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3838..3942
                     /label=AmpR promoter
     CDS             3943..4800
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4974..5562
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5850..5871
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5886..5916
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5924..5940
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 caggaaacag ctatgaccat gattacgcca agcttgttta aacatttaaa tctcgagcca
       61 tggattcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
      121 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      181 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      241 atagggactt tccattgacg tcaatgggtg gactatttac ggtaaactgc ccacttggca
      301 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      361 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      421 tacgtattag tcatcgctat taccatgggt cgaggtgagc cccacgttct gcttcactct
      481 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
      541 tgcagcgatg ggggcggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg
      601 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
      661 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
      721 gggcgggagt cgctgcgttg ccttcgcccc gtgccccgct ccgcgccgcc tcgcgccgcc
      781 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      841 ctccgggctg taattagcgc ttggtttaat gacggctcgt ttcttttctg tggctgcgtg
      901 aaagccttaa agggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg
      961 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc
     1021 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga gcgcggccgg
     1081 gggcggtgcc ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     1141 tgcgtggggg ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc cccccctgca
     1201 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtgcggggcg
     1261 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1321 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccg gagcgccggc
     1381 ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg
     1441 cagggacttc ctttgtccca aatctggcgg agccgaaatc tgggaggcgc cgccgcaccc
     1501 cctctagcgg gcgcgggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     1561 ccttcgtgcg tcgccgcgcc gccgtcccct tctccatctc cagcctcggg gctgccgcag
     1621 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1681 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1741 ggcaacgtgc tggttgttgt gctgtctcat cattttggca aagaattatc gcatgcctgc
     1801 agagctctag agtccgccca gtagtggctc cagagcccac aggtggcccc cggcagtctc
     1861 tggggcgcat ggagcggcgt taatagggct ggcggcgcag gccagtagcc gctccaacat
     1921 gaacctcgtg ggcagctacg cacaccatca ccaccatcac cacccgcacc ctgcgcaccc
     1981 catgctccac gaacccttcc tcttcggtcc ggcctcgcgc tgtcatcagg aaaggcccta
     2041 cttccagagc tggctgctga gcccggctga cgctgccccg gacttccctg cgggcgggcc
     2101 gccgcccgcg gccgctgcag ccgccaccgc ctatggtcct gacgccaggc ctgggcagag
     2161 ccccgggcgg ctggaggcgc ttggcggccg tcttggccgg cggaaaggct caggacccaa
     2221 gaaggagcgg agacgcactg agagcattaa cagcgcattc gcggagttgc gcgagtgcat
     2281 ccccaacgtg ccggccgaca ccaagctctc caagatcaag actctgcgcc tagccaccag
     2341 ctacatcgcc tacctgatgg acgtgctggc caaggatgca cagtctggcg atcccgaggc
     2401 cttcaaggct gaactcaaga aggcggatgg cggccgtgag agcaagcgga aaagggagct
     2461 gcagcagcac gaaggttttc ctcctgccct gggcccagtc gagaagagga ttaaaggacg
     2521 caccggctgg ccgcagcaag tctgggcgct ggagttaaac cagtgagccg aggcccgcgc
     2581 cgaggacctg gccaggccag ccactcctga agccccggga ggagaggaag gcagcggcga
     2641 acgccaggct ctgggctccg gcgactggtg ctacgcatcc cgcggagctt ctgctgagcg
     2701 ccggcaggtc gtcggctgca accacgacgg gcccggtacc gcggccgcac ttaagttacg
     2761 cgtggatcaa ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc
     2821 caatgccctg gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga
     2881 catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc
     2941 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
     3001 tttaaaacat cagaatgagt atttggttta gagtttggca acatatgcca tatgctggct
     3061 gccatgaaca aaggtggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc
     3121 attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg
     3181 tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt
     3241 tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatga agatccctcg
     3301 acctgcagcc caagctgatc ccgggattta aatgtttaaa cgaattcact ggccgtcgtt
     3361 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     3421 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     3481 ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc
     3541 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     3601 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     3661 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     3721 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     3781 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     3841 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     3901 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     3961 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     4021 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     4081 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     4141 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     4201 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     4261 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     4321 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     4381 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     4441 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     4501 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     4561 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     4621 tggtttattg ctgataaatc tggagcccgt gagcgtgggt ctcgcggtat cattgcagca
     4681 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     4741 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     4801 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     4861 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     4921 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     4981 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     5041 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     5101 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     5161 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     5221 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     5281 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     5341 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     5401 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     5461 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     5521 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     5581 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     5641 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     5701 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     5761 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     5821 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     5881 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     5941 tttcaca
//
