LOCUS       pTurboRFP-B.        4102 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expressing TurboRFP in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    pTurboRFP-B
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Evrogen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..54
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     protein_bind    62..78
                     /label=lac repressor encoded by lacI binding site
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             114..116
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             132..824
                     /label=TurboRFP
                     /note="red fluorescent protein from Entacmaea quadricolor"
     terminator      848..942
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             986..1642
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1710..1796
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     misc_feature    1952..2092
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(2278..2866)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3040..3897)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3898..4002)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
       61 attgtgagcg gataacaatt tcacacagaa ttgattaaag aggagaaatt aatatgagag
      121 gatcgggatc catgagcgag ctgatcaagg agaacatgca catgaagctg tacatggagg
      181 gcaccgtgaa caaccaccac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg
      241 gcacccagac catgaagatc aaggtggtcg agggcggccc tctccccttc gccttcgaca
      301 tcctggctac cagcttcatg tacggcagca aagccttcat caaccacacc cagggcatcc
      361 ccgacttctt taagcagtcc ttccctgagg gcttcacatg ggagagaatc accacatacg
      421 aagacggggg cgtgctgacc gctacccagg acaccagctt ccagaacggc tgcatcatct
      481 acaacgtcaa gatcaacggg gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa
      541 cacgcggctg ggaggccaac accgagatgc tgtaccccgc tgacggcggc ctgagaggcc
      601 acagccagat ggccctgaag ctcgtgggcg ggggctacct gcactgctcc ttcaagacca
      661 catacagatc caagaaaccc gctaagaacc tcaagatgcc cggcttccac ttcgtggacc
      721 acagactgga aagaatcaag gaggccgaca aagagaccta cgtcgagcag cacgagatgg
      781 ctgtggccaa gtactgcgac ctccctagca aactggggca cagatgaagc ttaattagct
      841 gagcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc
      901 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaagcta gcttggcgag
      961 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga
     1021 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac
     1081 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa
     1141 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga
     1201 atttcgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta
     1261 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga
     1321 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc
     1381 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag
     1441 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac
     1501 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca
     1561 tcatgccgtc tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg
     1621 cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt tattggtgcc cttaaacgcc
     1681 tggggtaatg actctctagc ttgaggcatc aaataaaacg aaaggctcag tcgaaagact
     1741 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc
     1801 cgctctagag ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     1861 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     1921 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
     1981 gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
     2041 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
     2101 cgcttcctcg ctcactgact cgctgcgctc ggtctgtcgg ctgcggcgag cggtatcagc
     2161 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     2221 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     2281 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     2341 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     2401 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     2461 ggcgctttct caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     2521 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     2581 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     2641 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     2701 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     2761 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     2821 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     2881 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     2941 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     3001 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     3061 acctatctca gcgatctgtc tatttcgttc atccatagct gcctgactcc ccgtcgtgta
     3121 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     3181 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     3241 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     3301 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     3361 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     3421 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     3481 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     3541 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     3601 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     3661 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     3721 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     3781 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     3841 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     3901 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     3961 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     4021 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     4081 cacgaggccc tttcgtcttc ac
//
