LOCUS       40924_44459        4130 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Promoterless TurboGFP reporter vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Evrogen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Can be used to monitor transcription from a promoter inserted into 
            the MCS.
FEATURES             Location/Qualifiers
     source          1..4130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    9..89
                     /label=MCS
                     /note="multiple cloning site"
     CDS             97..792
                     /codon_start=1
                     /label=TurboGFP
                     /note="improved green fluorescent protein from Pontellina 
                     plumata (Evdokimov et al., 2006)"
                     /translation="MESDESGLPAMEIECRITGTLNGVEFELVGGGEGTPEQGRMTNKM
                     KSTKGALTFSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVL
                     HVSFSYRYEAGRVIGDFKVMGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNDLDGSFTR
                     TFSLRDGGYYSSVVDSHMHFKSAIHPSILQNGGPMFAFRRVEEDHSNTELGIVEYQHAF
                     KTPDADAGEE"
     polyA_signal    918..1039
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1046..1501)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1528..1632
                     /label=AmpR promoter
     promoter        1634..1991
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2026..2817
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3052..3099
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3428..4016
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattac tagcgctacc ggactcagat ctcgagctca agcttcgaat tctgcagtcg
       61 acggtaccgc gggcccggga tccaccggtc gccaccatgg agagcgacga gagcggcctg
      121 cccgccatgg agatcgagtg ccgcatcacc ggcaccctga acggcgtgga gttcgagctg
      181 gtgggcggcg gagagggcac ccccgagcag ggccgcatga ccaacaagat gaagagcacc
      241 aaaggcgccc tgaccttcag cccctacctg ctgagccacg tgatgggcta cggcttctac
      301 cacttcggca cctaccccag cggctacgag aaccccttcc tgcacgccat caacaacggc
      361 ggctacacca acacccgcat cgagaagtac gaggacggcg gcgtgctgca cgtgagcttc
      421 agctaccgct acgaggccgg ccgcgtgatc ggcgacttca aggtgatggg caccggcttc
      481 cccgaggaca gcgtgatctt caccgacaag atcatccgca gcaacgccac cgtggagcac
      541 ctgcacccca tgggcgataa cgatctggat ggcagcttca cccgcacctt cagcctgcgc
      601 gacggcggct actacagctc cgtggtggac agccacatgc acttcaagag cgccatccac
      661 cccagcatcc tgcagaacgg gggccccatg ttcgccttcc gccgcgtgga ggaggatcac
      721 agcaacaccg agctgggcat cgtggagtac cagcacgcct tcaagacccc ggatgcagat
      781 gccggtgaag aataaagcgg ccgcgactct agatcataat cagccatacc acatttgtag
      841 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
      901 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
      961 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     1021 aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg
     1081 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     1141 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     1201 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     1261 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     1321 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     1381 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     1441 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     1501 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     1561 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     1621 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
     1681 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     1741 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     1801 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     1861 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     1921 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     1981 gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     2041 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     2101 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     2161 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg
     2221 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     2281 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     2341 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     2401 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     2461 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     2521 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc
     2581 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     2641 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     2701 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     2761 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     2821 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     2881 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     2941 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga
     3001 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
     3061 aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
     3121 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
     3181 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
     3241 tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag attgatttaa
     3301 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     3361 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3421 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3481 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3541 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     3601 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3661 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3721 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3781 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     3841 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3901 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3961 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4021 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     4081 ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
//
