LOCUS       62056_21550        5009 bp DNA     circular SYN 28-AUG-2019
DEFINITION  Cloning vector pTrichoGate-9, complete sequence.
ACCESSION   MN007113
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Nogueira-Lopez G.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2019) BPRC, Lincoln University, Ellesmere Jct Rd, 
            Lincoln, Christchurch, Canterbury 7647, New Zealand
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (31-MAY-2019) BPRC, Lincoln University, Ellesmere Jct Rd, Lincoln, 
            Christchurch, Canterbury 7647, New Zealand"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..717
                     /label=EGFP
                     /note="enhanced GFP"
     misc_feature    1432..1467
                     /label=flexible linker GSAGSAAGSGEF
                     /note="flexible linker GSAGSAAGSGEF"
     promoter        complement(1551..1569)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1576..1592)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             1730..2029
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             2381..3172
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             3382..3753
                     /label=BleoR
                     /note="antibiotic-binding protein"
     rep_origin      3894..4482
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4770..4791
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4806..4836
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4844..4860
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4868..4884
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4902..4920
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 atggtatcaa agggcgaaga gctgttcact ggtgtcgttc ccatcctggt cgaattggac
       61 ggcgatgtca acggtcataa gttctccgtt agcggcgagg gtgaaggcga cgctacctac
      121 ggcaagctca ccttgaagtt catctgcacc actggcaagc tccccgtccc ttggccaacc
      181 ctcgttacca ctctgactta cggcgttcag tgcttttctc gctaccccga ccacatgaag
      241 caacatgatt tctttaagtc cgccatgcct gagggttacg tccaggaacg caccatcttc
      301 tttaaggacg atggcaacta taagacccga gctgaggtca agttcgaagg tgacaccctc
      361 gtcaaccgta tcgagctgaa gggcattgac tttaaggaag atggtaacat tcttggccac
      421 aagttggagt acaactataa ctcacataac gtctacatca tggccgataa gcagaagaac
      481 ggcattaagg tcaacttcaa gatccgccac aacattgagg acggctccgt ccaactggct
      541 gatcattacc agcaaaacac ccctatcggt gacggccctg tcctcctgcc tgataaccac
      601 tatctttcta ctcagagcgc cttgtccaag gaccccaacg agaagcgaga tcacatggtc
      661 ctcctggagt tcgttaccgc cgctggtatc actctcggca tggacgagct gtacaaggtt
      721 tctaagggcg aggaactctt caccggcgtc gttcctattc tcgtcgaact ggacggcgac
      781 gtcaacggcc acaagttttc tgtcagcggc gagggcgagg gcgacgccac ctacggcaag
      841 ctcaccctga agttcatttg caccactggc aagctgcccg tcccttggcc taccctcgtt
      901 actactctga cttacggcgt ccagtgcttc tcacgctatc cagatcacat gaagcagcat
      961 gatttcttta agtctgccat gcccgagggc tatgttcaag agcgtactat cttctttaag
     1021 gatgacggta actacaagac ccgagctgaa gttaagtttg agggtgacac cctcgtcaac
     1081 agaatcgagc ttaagggtat tgacttcaag gaggacggca acatcctcgg tcataagttg
     1141 gaatacaact ataactctca taacgtttat attatggctg acaaacagaa gaacggcatt
     1201 aaggtcaact ttaagatccg tcataacatc gaggacggca gcgttcaact cgccgaccac
     1261 tatcaacaga acacccctat cggcgacggt ccagtcctcc tgcccgataa ccactatctc
     1321 tcaactcagt ctgccctgtc taaggacccc aatgagaagc gcgaccacat ggttctcctg
     1381 gagttcgtca ccgccgctgg tattaccctt ggcatggacg agttgtataa gggttctgct
     1441 ggttctgctg ctggttctgg tgaattcgct atgagacctt aagggcgaat tctgcagata
     1501 tccatcacac tggcggccgc tcgagcatgc atctagaggg cccaattcgc cctatagtga
     1561 gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     1621 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     1681 ggcccgcacc gatcgccctt cccaacagtt gcgcagccta tacgtacggc agtttaaggt
     1741 ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat
     1801 tgacacgccg gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa
     1861 agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac
     1921 caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca
     1981 ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg
     2041 catgagatta tcaaaaagga tcttcaccta gatccttttc acgtagaaag ccagtccgca
     2101 gaaacggtgc tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc
     2161 aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc
     2221 ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg
     2281 gaagccctgc aaagtaaact ggatggcttt ctcgccgcca aggatctgat ggcgcagggg
     2341 atcaagctct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt
     2401 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
     2461 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
     2521 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct
     2581 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
     2641 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
     2701 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
     2761 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
     2821 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
     2881 agccgaactg ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac
     2941 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     3001 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     3061 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     3121 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaattat
     3181 taacgcttac aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     3241 ccgcatacag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     3301 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     3361 tagcacgtga ggagggccac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
     3421 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
     3481 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
     3541 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
     3601 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
     3661 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
     3721 aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctaaaact tcatttttaa
     3781 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     3841 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     3901 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     3961 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     4021 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     4081 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     4141 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     4201 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     4261 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     4321 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     4381 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     4441 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     4501 tttttacggt tcctgggctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     4561 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     4621 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     4681 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     4741 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     4801 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     4861 tttcacacag gaaacagcta tgaccatgat tacgccaagc tatttaggtg acactataga
     4921 atactcaagc tatgcatcaa gcttggtacc gagctcggat ccactagtaa cggccgccag
     4981 tgtgctggaa ttcgccctta aggtctcta
//
