LOCUS       62056_21515        4117 bp DNA     circular SYN 28-AUG-2019
DEFINITION  Cloning vector pTrichoGate-21, complete sequence.
ACCESSION   MN007125
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Nogueira-Lopez G.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2019) BPRC, Lincoln University, Ellesmere Jct Rd, 
            Lincoln, Christchurch, Canterbury 7647, New Zealand
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (31-MAY-2019) BPRC, Lincoln University, Ellesmere Jct Rd, Lincoln, 
            Christchurch, Canterbury 7647, New Zealand"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        11..361
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     CDS             366..1388
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLEPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(1451..1467)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1941..2045
                     /label=AmpR promoter
     CDS             2046..2903
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3077..3665
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3953..3974
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3989..4019
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4027..4043
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4051..4067
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggtctctaat gtgatattga aggagcattt ttgggcttgg ctggagctag tggaggtcaa
       61 cacatcaatg ctattttggt ttagtcgtcc aggcggatca caaaatttgt gtcgtttgac
      121 aagatggttc atttaggcaa ctggtcagat cagcccactt gtaagcagta gcggcggcgc
      181 tcgaagtgtg actcttatta gcagacagga acgaggacat tattatcatc tgctgcttgg
      241 tgcacgataa cttgtgcgtt tgtcaagcaa ggtaagtgaa cgacccggtc ataccttctt
      301 aagttcgccc ttcctccctt tatttcagat tcaatctgac ttacctattc tacccaagca
      361 tccaaatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt
      421 tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct
      481 tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca
      541 aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg
      601 acattgggga gttcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca
      661 cgttgcaaga cctgcctgaa accgaactgc ccgctgttct ggagccggtc gcggaggcca
      721 tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc
      781 aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg
      841 tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg
      901 atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt
      961 tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg
     1021 aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt
     1081 tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat
     1141 cgccgcgcct ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg
     1201 ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat
     1261 ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg
     1321 atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg
     1381 caaaggaata gagtagatgc cgaccgggtt ccgttaacgg gcttagagac cgggtaccga
     1441 gctcgaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     1501 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     1561 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt
     1621 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
     1681 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     1741 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc atctccggga
     1801 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagtcga aagggcctcg
     1861 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     1921 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     1981 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     2041 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     2101 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     2161 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     2221 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     2281 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     2341 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     2401 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     2461 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     2521 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     2581 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     2641 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     2701 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     2761 gttctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     2821 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     2881 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     2941 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     3001 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     3061 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     3121 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     3181 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     3241 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     3301 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     3361 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     3421 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     3481 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     3541 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     3601 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     3661 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     3721 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     3781 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     3841 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     3901 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     3961 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     4021 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     4081 agcttgcatg cctgcaggtc gactctagag gatcccc
//
