LOCUS       62056_21510        4534 bp DNA     circular SYN 28-AUG-2019
DEFINITION  Cloning vector pTrichoGate-20, complete sequence.
ACCESSION   MN007124
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4534)
  AUTHORS   Nogueira-Lopez G.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2019) BPRC, Lincoln University, Ellesmere Jct Rd, 
            Lincoln, Christchurch, Canterbury 7647, New Zealand
REFERENCE   2  (bases 1 to 4534)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (31-MAY-2019) BPRC, Lincoln University, Ellesmere Jct Rd, Lincoln, 
            Christchurch, Canterbury 7647, New Zealand"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4534
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(35..602)
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus
                     nidulans trpC gene"
     CDS             complement(760..1551)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      complement(1552..1836)
                     /label=tryptophan synthase promoter (PtrpC)
                     /note="tryptophan synthase promoter (PtrpC)"
                     /regulatory_class="promoter"
     primer_bind     complement(1899..1915)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1923..1939)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1947..1977)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1992..2013)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2301..2889)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3063..3920)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3921..4025)
                     /label=AmpR promoter
     primer_bind     4499..4515
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggtctcttaa cctcgactct agaggatcct ctagaaagaa ggattacctc taaacaagtg
       61 tacctgtgca ttctgggtaa acgactcata ggagagttgt aaaaaagttt cggccggcgt
      121 attgggtgtt acggagcatt cactaggcaa ccatggttac tattgtatac ccatcttagt
      181 aggaatgatt ttcgaggttt atacctacga tgaatgtgtg tcctgtaggc ttgagagttc
      241 aaggaagaaa cagtgcaatt atctttgcga acccaggggc tggtgacgga attttcatag
      301 tcaagctatc agagtaaaga agaggagcat gtcaaagtac aattagagac aaatatatag
      361 tcgcgtggag ccaagagcgg attcctcagt ctcgtaggtc tcttgacgac cgttgatctg
      421 cttgatctcg tctcccgaaa atgaaaatag actctgctaa gctattcttc tgcttcgccg
      481 gagcctgaag ggcgtactag ggttgcgagg tccaatgcat taatgcattg cagatgagct
      541 gtatctggaa gaggtaaacc cgaaacgcgt tttattcttg ttgacatgga gctattaaat
      601 cactagaagg cactctttgc tgcttggaca aatgaacgta tcttatcgag atcctgaaca
      661 ccatttgtct caactccgga gctgacatcg acaccaacga tcttatatcc agattcgtca
      721 agctgtttga tgatttcagt aacgttaagt ggatcctcag aagaactcgt caagaaggcg
      781 atagaaggcg atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc
      841 agcccattcg ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata
      901 gcggtccgcc acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac
      961 catgatattc ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat
     1021 gcgcgccttg agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag
     1081 atcatcctga tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt
     1141 cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc
     1201 agccatgatg gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg
     1261 cacttcgccc aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc
     1321 gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt
     1381 cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg
     1441 gaacacggcg gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct
     1501 ctccacccaa gcggccggag aacctgcgtg caatccatct tgttcaatca tatcgatgct
     1561 tgggtagaat aggtaagtca gattgaatct gaaataaagg gaggaagggc gaacttaaga
     1621 aggtatgacc gggtcgttca cttaccttgc ttgacaaacg caccaagtta tcgtgcacca
     1681 agcagcagat gataataatg tcctcgttcc tgtctgctaa taagagtcac acttcgagcg
     1741 ccgccgctac tgctacaagt ggggctgatc tgaccagttg cctaaatgaa ccatcttgtc
     1801 aaacgacaca aattttgtgc tcaccgcctg gacgacgatg ttgagaccgg ggatcctcta
     1861 gagtcgacct gcaggcatgc aagcttggcg taatcatggt catagctgtt tcctgtgtga
     1921 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     1981 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     2041 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     2101 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     2161 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     2221 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     2281 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     2341 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2401 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     2461 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     2521 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     2581 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     2641 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     2701 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     2761 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     2821 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     2881 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     2941 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3001 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     3061 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     3121 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     3181 agtgctgcaa tgataccgcg agaaccacgc tcaccggctc cagatttatc agcaataaac
     3241 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     3301 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     3361 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     3421 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     3481 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     3541 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     3601 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     3661 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     3721 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     3781 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     3841 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     3901 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     3961 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     4021 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     4081 ttaacctata aaaataggcg tatcacgagg ccctttcgac tcgcgcgttt cggtgatgac
     4141 ggtgaaaacc tctgacacat gcagctcccg gagatggtca cagcttgtct gtaagcggat
     4201 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
     4261 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     4321 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc
     4381 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     4441 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     4501 aaaacgacgg ccagtgaatt cgagctcggt accc
//
