LOCUS       62056_21500        5968 bp DNA     circular SYN 28-AUG-2019
DEFINITION  Cloning vector pTrichoGate-19, complete sequence.
ACCESSION   MN007123
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5968)
  AUTHORS   Nogueira-Lopez G.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2019) BPRC, Lincoln University, Ellesmere Jct Rd, 
            Lincoln, Christchurch, Canterbury 7647, New Zealand
REFERENCE   2  (bases 1 to 5968)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (31-MAY-2019) BPRC, Lincoln University, Ellesmere Jct Rd, Lincoln, 
            Christchurch, Canterbury 7647, New Zealand"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            1..2445
                     /gene="CbxR"
                     /label=CbxR
     regulatory      1..961
                     /gene="CbxR"
                     /label=succinate dehydrogenase promoter region (sdhP)
                     /note="succinate dehydrogenase promoter region (sdhP)"
                     /regulatory_class="promoter"
     CDS             join(962..1465,1536..1862)
                     /codon_start=1
                     /transl_table=11
                     /gene="CbxR"
                     /product="succinate dehydrogenase"
                     /label=CbxR
                     /note="sdh; carboxin resistance gene"
                     /protein_id="QEG99044.1"
                     /translation="MAALRSSTRILSSSTRAAFRPSAVFSRSMASVSETTEQQPRVKSF
                     QIYRWNPDTPTEKPRLQTYSIDLNKTGPMILDALIKIKNEQDPSLTFRRSCREGICGSC
                     AMNINGQNTLACLCRIPTESASDVKIYPLPHTYVVKDLVPDLTHFYKQYKSIQPYLQRD
                     TPAEDGKEYRQSKADRKKLDGLYECILCACCSTSCPSYWWNSEEYLGPAILLQSYRWLI
                     DSRDERTEARKSKLENSMSLYRCLTILNCTRACPKGLNPGKAIAEIKKQMSLGA"
     intron          1466..1535
                     /gene="CbxR"
     regulatory      1863..2445
                     /gene="CbxR"
                     /label=succinate dehydrogenase terminator region (sdhT)
                     /note="succinate dehydrogenase terminator region (sdhT)"
                     /regulatory_class="terminator"
     promoter        complement(2518..2536)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2543..2559)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2697..2996
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             3348..4139
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             4349..4720
                     /label=BleoR
                     /note="antibiotic-binding protein"
     rep_origin      4861..5449
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5737..5758
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5773..5803
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5811..5827
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5835..5851
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5869..5887
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 ggtctcaaca tgtgtctgta tgttaaaaac tgcgacgcct ggcaagtcct gggtgcgcaa
       61 ctagaggcta ttcggacggt tagcaggcaa ggcgatgttg gcgacacaaa gtcgcgtcac
      121 gcagggccac aaaccattca acagcgttcg cagtgatggc gaggcagtca atctgccaga
      181 gaaaaacatg gccgtctagg aaatgtcgtc tgcgatggat tgtcgcaaag aaaaaacgag
      241 catcgatggg cgaggcagtc cgcagcgggc gcgcagtcgc caatcagggc aatactttag
      301 acaagcagct tcttcgccat tccatagcca caatccgggc tcttcacggt catgcggcca
      361 agtcgtcaga aagccgcccg cgccgaaaaa aaaagaaaaa attactgctg aaattaaagg
      421 tgaggcaaac aaactctaca gtatgggcga taacaaaagg attcaatcgg gattattaaa
      481 ccccaccaaa atccccaaaa tcttccccca cgaccatttg cgcgctaagg cacgtaatga
      541 ttagctaatt ttccaagtcg gcacgtgcaa gggtctgcag ctgctggccc gggctttagc
      601 cggctgtcac gagctgccgc ctggcaattc cgactttctc ctgcttagcc gaattgtcaa
      661 tgaaacgact gtgattggct ggcccgtggc cgtgaatcga ggtcacatag ccgaggaaga
      721 gccacttcca tcttcccagc ttcttccatg acgacgagtc ctcgagccgc caccgagtat
      781 ctttgcaatt gagcggctgc caggaggaac caagagcttc gattgccttg gctcgaatca
      841 atcacctgct gcaaatccga tccctccctg tgagcaactg tagcgcagct tcaaacgcgg
      901 ggctcccgga tctgaaaaaa gcaatcccga gcaaaagcat cgacacgaga caaccgcaac
      961 catggccgct ctccgctcat ctactcgaat cctcagctcc tctacgaggg cagctttccg
     1021 acccagcgcc gtcttctcgc gctccatggc gtcggtcagc gaaaccactg agcagcagcc
     1081 cagagtcaag tcgttccaga tctaccgatg gaatcccgat accccaaccg agaagccgcg
     1141 gcttcagacc tactccattg acctcaacaa gacgggcccc atgatcttgg acgccctgat
     1201 caagatcaag aacgagcagg acccgtcgct gaccttccgg agaagctgcc gagagggcat
     1261 ctgcggcagc tgtgccatga acatcaacgg ccagaacacc ctggcttgct tgtgccgaat
     1321 ccccaccgag tctgcctccg atgtcaagat ctaccctctg ccgcacacct acgtcgtcaa
     1381 ggacctggtg cccgacttga cgcacttcta caagcaatac aagtccatcc agccctacct
     1441 gcagcgcgat acccctgctg aagacgtatg ccggcatccc gatctaacct gacccttgtg
     1501 tagtttgagc aagctttgct aactataaga aacagggcaa ggagtaccgc cagagcaagg
     1561 cggatcgaaa gaagctggac ggactctacg agtgcattct gtgcgcctgc tgctcaacct
     1621 cgtgcccctc gtactggtgg aattccgaag agtacctcgg ccccgccatc ctgctgcagt
     1681 cataccgatg gctgattgac tcccgagacg agcgcacgga ggcccgcaag tccaagctcg
     1741 agaactcaat gagcctgtac cgttgcctca ccatcctcaa ctgcacgcgt gcttgcccca
     1801 agggcctgaa ccccggcaag gccattgccg agatcaagaa gcaaatgtct cttggagcat
     1861 aaagagcgaa ttaggttgtt attggggggg aatgaatagg aaacaaaaat tgggcggata
     1921 acttgaggat tcactgccgc tgtgtattaa tcaccgatgg aattaccatc tatcgatgtt
     1981 cgactaggga gaagagtaaa aatatctttt ttttacataa ccagttcatt gtactctcgc
     2041 aatgtttcat tttgtctagc agtctgtgat tccgactctc cgccttggcg acagcaaatg
     2101 gtgaaatgtc acgagtcggt gcattccttg gccagtccat tctgaaaaca ccagcatact
     2161 cagtccgacg ccaccgtttc aaccagcctc tcccttgcac aaagtcggtc cgtcccctta
     2221 caccacatgg ccttttctcg tcgcacaaaa gccgtctcca tcccggctga tgtcgcaaga
     2281 ggcgtggcag gcacgacttt acgctgtttg tttgtataaa ttagcgtgca gatcttttag
     2341 agcaagtccc agatcatcat tcgtccctca agcgtattac ctattcctat tattagtatc
     2401 atttattctc cgagagatct tgttcgttac gacggttaag agaccttaag ggcgaattct
     2461 gcagatatcc atcacactgg cggccgctcg agcatgcatc tagagggccc aattcgccct
     2521 atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     2581 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     2641 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctatac gtacggcagt
     2701 ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg
     2761 atattattga cacgccgggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt
     2821 cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca
     2881 tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc
     2941 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa
     3001 tgtcaggcat gagattatca aaaaggatct tcacctagat ccttttcacg tagaaagcca
     3061 gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc tggacaaggg
     3121 aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag
     3181 actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta
     3241 aggttgggaa gccctgcaaa gtaaactgga tggctttctc gccgccaagg atctgatggc
     3301 gcaggggatc aagctctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     3361 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     3421 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     3481 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
     3541 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     3601 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     3661 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     3721 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     3781 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     3841 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg
     3901 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     3961 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     4021 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     4081 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     4141 gaattattaa cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta
     4201 tttcacaccg catacaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     4261 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     4321 tcaataatag cacgtgagga gggccaccat ggccaagttg accagtgccg ttccggtgct
     4381 caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg
     4441 ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag
     4501 cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct
     4561 ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg
     4621 gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc
     4681 ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc taaaacttca
     4741 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     4801 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     4861 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4921 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4981 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     5041 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     5101 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     5161 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     5221 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     5281 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     5341 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     5401 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     5461 cgcggccttt ttacggttcc tgggcttttg ctggcctttt gctcacatgt tctttcctgc
     5521 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     5581 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     5641 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     5701 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     5761 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     5821 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat ttaggtgaca
     5881 ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca ctagtaacgg
     5941 ccgccagtgt gctggaattc gcccttaa
//
