LOCUS       62056_21465        5724 bp DNA     circular SYN 28-AUG-2019
DEFINITION  Cloning vector pTrichoGate-12, complete sequence.
ACCESSION   MN007116
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5724)
  AUTHORS   Nogueira-Lopez G.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2019) BPRC, Lincoln University, Ellesmere Jct Rd, 
            Lincoln, Christchurch, Canterbury 7647, New Zealand
REFERENCE   2  (bases 1 to 5724)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (31-MAY-2019) BPRC, Lincoln University, Ellesmere Jct Rd, Lincoln, 
            Christchurch, Canterbury 7647, New Zealand"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..717
                     /label=EGFP
                     /note="enhanced GFP"
     promoter        complement(2228..2246)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2253..2269)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3058..3849
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     CDS             4059..4430
                     /label=BleoR
                     /note="antibiotic-binding protein"
     rep_origin      4571..5159
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5447..5468
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5483..5513
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5521..5537
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5545..5561
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5579..5597
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    5689..5720
                     /label=flexible linker GSAGSAAGSGEF
                     /note="flexible linker GSAGSAAGSGEF"
ORIGIN
        1 atggtatcaa agggcgaaga gctgttcact ggtgtcgttc ccatcctggt cgaattggac
       61 ggcgatgtca acggtcataa gttctccgtt agcggcgagg gtgaaggcga cgctacctac
      121 ggcaagctca ccttgaagtt catctgcacc actggcaagc tccccgtccc ttggccaacc
      181 ctcgttacca ctctgactta cggcgttcag tgcttttctc gctaccccga ccacatgaag
      241 caacatgatt tctttaagtc cgccatgcct gagggttacg tccaggaacg caccatcttc
      301 tttaaggacg atggcaacta taagacccga gctgaggtca agttcgaagg tgacaccctc
      361 gtcaaccgta tcgagctgaa gggcattgac tttaaggaag atggtaacat tcttggccac
      421 aagttggagt acaactataa ctcacataac gtctacatca tggccgataa gcagaagaac
      481 ggcattaagg tcaacttcaa gatccgccac aacattgagg acggctccgt ccaactggct
      541 gatcattacc agcaaaacac ccctatcggt gacggccctg tcctcctgcc tgataaccac
      601 tatctttcta ctcagagcgc cttgtccaag gaccccaacg agaagcgaga tcacatggtc
      661 ctcctggagt tcgttaccgc cgctggtatc actctcggca tggacgagct gtacaaggtt
      721 tctaagggcg aggaactctt caccggcgtc gttcctattc tcgtcgaact ggacggcgac
      781 gtcaacggcc acaagttttc tgtcagcggc gagggcgagg gcgacgccac ctacggcaag
      841 ctcaccctga agttcatttg caccactggc aagctgcccg tcccttggcc taccctcgtt
      901 actactctga cttacggcgt ccagtgcttc tcacgctatc cagatcacat gaagcagcat
      961 gatttcttta agtctgccat gcccgagggc tatgttcaag agcgtactat cttctttaag
     1021 gatgacggta actacaagac ccgagctgaa gttaagtttg agggtgacac cctcgtcaac
     1081 agaatcgagc ttaagggtat tgacttcaag gaggacggca acatcctcgg tcataagttg
     1141 gaatacaact ataactctca taacgtttat attatggctg acaaacagaa gaacggcatt
     1201 aaggtcaact ttaagatccg tcataacatc gaggacggca gcgttcaact cgccgaccac
     1261 tatcaacaga acacccctat cggcgacggt ccagtcctcc tgcccgataa ccactatctc
     1321 tcaactcagt ctgccctgtc taaggacccc aatgagaagc gcgaccacat ggttctcctg
     1381 gagttcgtca ccgccgctgg tattaccctt ggcatggacg agttgtacaa ggttagcaag
     1441 ggcgaggaac tttttaccgg cgtcgttccc attcttgtcg agttggatgg cgacgtcaac
     1501 ggtcacaagt tctccgtctc aggcgagggt gaaggcgatg caacctacgg caagctcacc
     1561 ctaaagttta tttgcactac cggcaagctg cccgtccctt ggccgaccct cgttacaact
     1621 ctgacttacg gcgtacagtg cttcagccgc tatcctgatc acatgaagca gcacgatttc
     1681 tttaagagcg ctatgccaga gggctatgtt caagaacgta ccatcttctt taaggatgat
     1741 ggtaattaca aaacccgagc tgaagtcaag tttgaaggtg acaccctcgt caacaggatt
     1801 gagctgaagg gtattgactt taaggaggac ggcaacattc tcggtcataa gttggagtat
     1861 aactacaact cccacaacgt ttatattatg gccgacaagc agaagaacgg cattaaggtc
     1921 aacttcaaaa tccgacataa catcgaagac ggctctgtcc aactcgccga ccactaccaa
     1981 caaaacactc ccatcggtga cggccctgtt ctcctgccag ataaccacta tctgtccact
     2041 cagtcagctc tttctaagga ccccaacgag aagcgtgatc acatggtcct ccttgagttt
     2101 gtcaccgctg ctggtatcac cttgggtatg gacgagttgt ataagcttag agaccttaag
     2161 ggcgaattct gcagatatcc atcacactgg cggccgctcg agcatgcatc tagagggccc
     2221 aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac
     2281 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     2341 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctatac
     2401 gtacggcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
     2461 gtacagagtg atattattga cacgccgggg cgacggatgg tgatccccct ggccagtgca
     2521 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa
     2581 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa
     2641 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
     2701 ggaatataaa tgtcaggcat gagattatca aaaaggatct tcacctagat ccttttcacg
     2761 tagaaagcca gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc
     2821 tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg
     2881 cgatagctag actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg
     2941 ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctc gccgccaagg
     3001 atctgatggc gcaggggatc aagctctgat caagagacag gatgaggatc gtttcgcatg
     3061 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     3121 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     3181 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa
     3241 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     3301 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     3361 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     3421 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     3481 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     3541 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc
     3601 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     3661 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     3721 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     3781 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     3841 gagttcttct gaattattaa cgcttacaat ttcctgatgc ggtattttct ccttacgcat
     3901 ctgtgcggta tttcacaccg catacaggtg gcacttttcg gggaaatgtg cgcggaaccc
     3961 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     4021 gataaatgct tcaataatag cacgtgagga gggccaccat ggccaagttg accagtgccg
     4081 ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg
     4141 ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc
     4201 tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg
     4261 tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg
     4321 acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc
     4381 tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc
     4441 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     4501 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     4561 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     4621 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     4681 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     4741 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     4801 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     4861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     4921 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     4981 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     5041 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     5101 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     5161 acgccagcaa cgcggccttt ttacggttcc tgggcttttg ctggcctttt gctcacatgt
     5221 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     5281 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     5341 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     5401 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     5461 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     5521 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
     5581 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca
     5641 ctagtaacgg ccgccagtgt gctggaattc gcccttaagg tctcagccgg atctgctggt
     5701 tctgctgctg gttctggtga attc
//
