LOCUS       40924_43978        5989 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Bacterial expression vector pTrcmelA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5989)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 5989)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5989)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(41..1870)
                     /codon_start=1
                     /note="unnamed protein product; melA"
                     /protein_id="SJL86559.1"
                     /translation="MAWLVGKPSLERSWNAILSFPESGFQLECRNTIGSSVFSSHFTLH
                     FRVARRLLHFSCRRFTETQKEPTQALWWCELPTAPAPRRRGTGLKAALILAKDNSNPRE
                     SKMSITRRHVIVQGGVIAAGLLASGLPGTKAFAQIPSIPWRRSLQGLAWNDPIIETYRD
                     AVRLLNALPASDKFNWVNLSKIHGSGDVVKYCPHGNWYFLPWHRAYTAMYERIVRHVTK
                     NNDFAMPFWDWTDNPYLPEVFTMQKTPDGKDNPLYVSSRTWPITQPMPDNIVGPQVLNT
                     ILTAKPYEVFGTTRPEGQNSLDPSWVTTSSGTQGALEYTPHNQVHNNIGGWMPEMSSPR
                     DPIFFMHHCNIDRIWATWNLRNANSTDRLWADMPFTDNFYDVDGNFWSPKVSDLYVPEE
                     LGYNYGFRTYFKVAAASAKTLALNDKLTSVIAATATDAAIAGVTTTSTDNSKAATENVP
                     LSLPIKIPAGALQEIVRQPPLPSGMDTMDFGAAQEQAASAPRVLAFLRDVEITSASTTS
                     VRVFLGKNDLKADTPVTDPHYVGSFAVLGHDGDHHRKPSFVLDLTDAIQRVYGGRGQTD
                     GEAIDLQLIPVGSGAGKPGAVEPAKLEIAIVSA"
     protein_bind    complement(1891..1907)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1915..1944)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     CDS             complement(2179..3258)
                     /label=lacI
                     /note="lac repressor"
     promoter        complement(3259..3336)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     misc_feature    3522..3662
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3848..4436)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4610..5467)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5468..5559)
                     /label=AmpR promoter
     terminator      complement(5579..5606)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(5698..5784)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 cttgcatgcc tgcaggtcga ctctagagga tccccgggtg ttaggcggac actatggcta
       61 tttctagctt tgcgggctct acggcgccgg gtttgcccgc tcccgatccg acaggaatga
      121 gctgcaggtc gatggcctcg ccatccgtct gcccccttcc gccgtaaacc cgctggatcg
      181 cgtccgtcag atcgaggacg aaggatggtt tgcgatgatg gtcgccgtca tgaccgagaa
      241 cggcgaaaga accgacgtaa tggggatcgg tgacgggcgt atcggcctta aggtcgttct
      301 tgccgagaaa cacccgaacg ctggtcgtgc tggcactggt gatctcgaca tcgcgcagga
      361 atgccagcac acgaggagcg gaggccgcct gctcctgtgc cgcgccgaaa tccattgtat
      421 ccatgccgga tggcagaggc ggttggcgga cgatctcctg caatgcgccc gccgggatct
      481 tgatcggcag cgaaagcggc acgttttccg ttgccgcctt gctgttgtcc gtggaggtgg
      541 tcgtcacgcc ggcgattgca gcatcggtcg ccgtcgccgc gatcacggac gtgagtttat
      601 cgttcagggc cagcgttttg gcgctcgccg ccgcgacctt gaagtaggtc cggaaaccat
      661 aattgtatcc gagttcctct ggaacataaa ggtcagagac cttcggggac cagaagttgc
      721 cgtcgacatc gtagaaattg tcggtgaacg gcatgtcggc ccagagtcga tccgtgctgt
      781 tggcgttgcg caaattccag gtcgcccaga tgcggtcgat gttgcaatga tgcatgaaga
      841 agatcggatc gcggggcgac gacatttccg gcatccagcc accgatattg ttgtgcacct
      901 gattgtgcgg tgtgtattcc agcgccccct gcgtgccgct gctggtggtg acccaggaag
      961 gatcgagtga gttctgtccc tcggggcggg tggtgccgaa gacctcgtat ggcttcgccg
     1021 ttaggatggt gttgagaacc tgtggcccaa ctatattgtc cggcatcggc tgcgtgattg
     1081 gccaggtgcg cgacgaaaca taaagtggat tgtccttgcc gtcgggcgtc ttttgcattg
     1141 tgaacacttc gggcaggtac ggattgtcgg tccagtccca gaacggcata gcgaaatcgt
     1201 tgttcttggt cacgtgccga acgatgcgct cgtacatagc cgtataggcc ctgtgccacg
     1261 gcaggaaata ccagttgcca tgcgggcagt atttgacgac gtcaccgctg ccgtgaattt
     1321 tcgagaggtt gacccagttg aatttgtcgc tggcgggaag ggcgttgaga aggcgcactg
     1381 cgtcgcgata ggtctcgatg atcgggtcgt tccaggccaa gccctgcagt gagcgccgcc
     1441 aagggattga cggtatctgc gcgaaggctt ttgtccccgg taggccgctg gcgagcaggc
     1501 ctgctgcaat gacgccaccc tgaacgatga catgtctgcg tgtgatgctc atcttactct
     1561 cccttggatt tgaattgtcc ttcgccaaga ttaatgccgc ttttaggccg gttccgcgcc
     1621 gtctgggcgc gggagcagtg ggcaattcgc accaccatag agcttgcgtt ggctctttct
     1681 gagtttcggt gaatcggcgg cacgagaaat gaagcaagcg acgtgccact cgaaaatgca
     1741 gcgtaaaatg cgagctaaaa acgctactac cgatcgtatt cctgcattcc agttggaatc
     1801 cagattccgg aaaacttagt atcgcattcc atgatcgttc gagcgacggc ttgccgacca
     1861 gccacgccat ggtctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacattat
     1921 acgagccgga tgattaattg tcaacagctc atttcagaat atttgccaga accgttatga
     1981 tgtcggcgca aaaaacatta tccagaacgg gagtgcgcct tgagcgacac gaattatgca
     2041 gtgatttacg acctgcacag ccataccaca gcttccgatg gctgcctgac gccagaagca
     2101 ttggtgcacc gtgcagtcga tgataagctg tcaaaccaga tcaattcgcg ctaactcaca
     2161 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     2221 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt
     2281 tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag
     2341 ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt
     2401 tgacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatatc
     2461 cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc
     2521 gttggcaacc agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg
     2581 aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg
     2641 agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc
     2701 cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt
     2761 accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa
     2821 taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg
     2881 atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca
     2941 ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc
     3001 gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc
     3061 aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta
     3121 attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc
     3181 ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta
     3241 taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc
     3301 cataccgcga aaggttttgc accattcgat ggtgtcaacg taaatgcatg ccgcttcgcc
     3361 ttcgcgcgcg aattgatctg ctgcctcgcg cgtttcggtg atgacggtga aaacctctga
     3421 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     3481 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca
     3541 cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga
     3601 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     3661 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     3721 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     3781 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     3841 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     3901 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     3961 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     4021 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     4081 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     4141 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     4201 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     4261 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     4321 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     4381 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     4441 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     4501 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     4561 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     4621 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     4681 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     4741 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     4801 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     4861 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     4921 ctgtaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     4981 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     5041 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     5101 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     5161 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     5221 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     5281 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     5341 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     5401 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     5461 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     5521 gcggatacat atttgaatgt atttagaaaa ataaacaaaa agagtttgta gaaacgcaaa
     5581 aaggccatcc gtcaggatgg ccttctgctt aatttgatgc ctggcagttt atggcgggcg
     5641 tcctgcccgc caccctccgg gccgttgctt cgcaacgttc aaatccgctc ccggcggatt
     5701 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttt
     5761 cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgca tggggagacc
     5821 ccacactacc atcggcgcta cggcgtttca cttctgagtt cggcatgggg tcaggtggga
     5881 ccaccgcgct actgccgcca ggcaaattct gttttatcag accgcttctg cgttctgatt
     5941 taatctgtat caggctgaaa atcttctctc atccgccaaa acagccaag
//
